Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   BSF20_RS05200 Genome accession   NZ_CP018152
Coordinates   1007774..1008211 (+) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain LM2303     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1002774..1013211
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSF20_RS05185 (BSF20_05085) - 1005632..1005994 (-) 363 WP_014304320.1 winged helix-turn-helix transcriptional regulator -
  BSF20_RS05190 (BSF20_05090) hxlA 1006226..1006861 (+) 636 WP_014304319.1 3-hexulose-6-phosphate synthase -
  BSF20_RS05195 (BSF20_05095) hxlB 1006858..1007415 (+) 558 WP_014304318.1 6-phospho-3-hexuloisomerase -
  BSF20_RS05200 (BSF20_05100) nucA/comI 1007774..1008211 (+) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  BSF20_RS05205 (BSF20_05105) nin/comJ 1008233..1008631 (+) 399 WP_014304317.1 competence protein ComJ Regulator
  BSF20_RS05210 (BSF20_05110) - 1008672..1008890 (-) 219 Protein_1016 family 1 glycosylhydrolase -
  BSF20_RS20845 - 1008889..1009167 (-) 279 WP_107890409.1 family 1 glycosylhydrolase -
  BSF20_RS05220 (BSF20_05115) - 1009109..1009384 (-) 276 WP_369719109.1 YckD family protein -
  BSF20_RS05225 (BSF20_05120) - 1009501..1009935 (-) 435 WP_057080003.1 RDD family protein -
  BSF20_RS05230 (BSF20_05125) - 1009950..1011086 (-) 1137 WP_003156594.1 zinc-dependent alcohol dehydrogenase -
  BSF20_RS05235 (BSF20_05130) - 1011151..1012353 (-) 1203 WP_072588306.1 GTP-binding protein -
  BSF20_RS20525 - 1012467..1012631 (+) 165 WP_370648487.1 molybdopterin dinucleotide binding domain-containing protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=206510 BSF20_RS05200 WP_003156588.1 1007774..1008211(+) (nucA/comI) [Bacillus amyloliquefaciens strain LM2303]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=206510 BSF20_RS05200 WP_003156588.1 1007774..1008211(+) (nucA/comI) [Bacillus amyloliquefaciens strain LM2303]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAAGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGGCATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAACAATGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641

Multiple sequence alignment