Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   CNQ52_RS00675 Genome accession   NZ_CP023366
Coordinates   146993..147661 (+) Length   222 a.a.
NCBI ID   WP_001297375.1    Uniprot ID   P65957
Organism   Escherichia coli strain 1428     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 141993..152661
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CNQ52_RS00650 (CNQ52_00650) pyrE 142518..143159 (+) 642 WP_000806177.1 orotate phosphoribosyltransferase -
  CNQ52_RS00655 (CNQ52_00655) - 143457..144416 (+) 960 WP_022646277.1 reverse transcriptase family protein -
  CNQ52_RS00660 (CNQ52_00660) slmA 144462..145058 (-) 597 WP_000818603.1 nucleoid occlusion factor SlmA -
  CNQ52_RS00665 (CNQ52_00665) dut 145165..145623 (-) 459 WP_000976070.1 dUTP diphosphatase -
  CNQ52_RS00670 (CNQ52_00670) coaBC 145601..146821 (-) 1221 WP_000050149.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  CNQ52_RS00675 (CNQ52_00675) radC 146993..147661 (+) 669 WP_001297375.1 DNA repair protein RadC Machinery gene
  CNQ52_RS00680 (CNQ52_00680) rpmB 147878..148114 (+) 237 WP_000091955.1 50S ribosomal protein L28 -
  CNQ52_RS00685 (CNQ52_00685) rpmG 148135..148302 (+) 168 WP_001051798.1 50S ribosomal protein L33 -
  CNQ52_RS00690 (CNQ52_00690) mutM 148400..149209 (+) 810 WP_001114533.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  CNQ52_RS00695 (CNQ52_00695) coaD 149248..149727 (-) 480 WP_001171866.1 pantetheine-phosphate adenylyltransferase -
  CNQ52_RS00700 (CNQ52_00700) waaA 149735..151012 (-) 1278 WP_000891574.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  CNQ52_RS00705 (CNQ52_00705) rfaQ 151425..152483 (+) 1059 WP_000360395.1 lipopolysaccharide core heptosyltransferase RfaQ -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25258.35 Da        Isoelectric Point: 7.9748

>NTDB_id=206441 CNQ52_RS00675 WP_001297375.1 146993..147661(+) (radC) [Escherichia coli strain 1428]
MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=206441 CNQ52_RS00675 WP_001297375.1 146993..147661(+) (radC) [Escherichia coli strain 1428]
GTGAAAAACAATGCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACGGATGTCGAGCT
GCTGGCGCTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAACAGTTTAGTGGCGTTCATGGAATTGGCGTGGCGAAATTCGCCCAGTTA
AAGGGGATTGCCGAACTGGCGCGGCGTTACTACAACGTGCGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GAGGGAATTTTTACAAAGCCAGCTTACGGGCGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCACAACACCGGG
TTATAACGCATAGCCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAATGCCTCGGCGCTGATCCTTGCACATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTAATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

RadC-like JAB domain (RadC)

(103-220)

UPF0758 N-terminal (UPF0758_N)

(9-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P65957

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496

Multiple sequence alignment