Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   BP422_RS27015 Genome accession   NZ_CP018145
Coordinates   5673542..5674909 (+) Length   455 a.a.
NCBI ID   WP_088910268.1    Uniprot ID   A0A220MP74
Organism   Brevibacillus formosus strain NF2     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 5668542..5679909
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BP422_RS26995 (BP422_26340) - 5668832..5669296 (+) 465 WP_047074759.1 CtsR family transcriptional regulator -
  BP422_RS27000 (BP422_26345) - 5669313..5669831 (+) 519 WP_088910265.1 UvrB/UvrC motif-containing protein -
  BP422_RS27005 (BP422_26350) - 5669851..5670924 (+) 1074 WP_088910266.1 protein arginine kinase -
  BP422_RS27010 (BP422_26355) - 5670985..5673435 (+) 2451 WP_088910267.1 ATP-dependent Clp protease ATP-binding subunit -
  BP422_RS27015 (BP422_26360) radA 5673542..5674909 (+) 1368 WP_088910268.1 DNA repair protein RadA Machinery gene
  BP422_RS27020 (BP422_26365) disA 5674918..5675994 (+) 1077 WP_088911038.1 DNA integrity scanning diadenylate cyclase DisA -
  BP422_RS27025 (BP422_26370) pssA 5676109..5676837 (+) 729 WP_088910269.1 CDP-diacylglycerol--serine O-phosphatidyltransferase -
  BP422_RS27030 (BP422_26375) - 5676934..5677329 (-) 396 WP_007716195.1 hypothetical protein -
  BP422_RS27035 (BP422_26380) - 5677616..5678704 (+) 1089 WP_088910270.1 PIN/TRAM domain-containing protein -
  BP422_RS27040 (BP422_26385) ispD 5678732..5679409 (+) 678 WP_088910271.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  BP422_RS27045 (BP422_26390) ispF 5679413..5679886 (+) 474 WP_088910272.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 49427.78 Da        Isoelectric Point: 6.8250

>NTDB_id=206390 BP422_RS27015 WP_088910268.1 5673542..5674909(+) (radA) [Brevibacillus formosus strain NF2]
MSKYKTKYACQECGYESPKWMGKCPGCSSWNTLVEEVTVKNAHRHEGLSGGQRQSAIPLTQVASEEEPRMDTTIGELNRV
LGGGLVPGSLILVGGDPGIGKSTLLLQTSFALAHQGAKVLYVSGEESAKQIKIRADRLHLQTPPMFVLAENDLDLIEQHI
NQVDPDVLIIDSIQTVFHPTIQSAAGSVAQVREATAQLMRIAKGKGIGTFIVGHVTKEGSIAGPRMLEHMVDAVLYFEGE
RHNTFRILRAVKNRFGSTNEIGIFEMKDRGLEEVANPSEIFLAERPFGVAGSTVVASMEGTRPVLVELQALVAPTSFVTP
RRTATGVDHQRVAMIMAVLEKRMGMMLQNQDAYVNVAGGVRLDEPAVDLAIAVSIASSFRDHATNPHDVVIGEIGLTGEV
RGVSRIEQRVREAHKLGFKRVIIPEKNIRGLDAPADIQVIGVSNIADALNEVIRR

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=206390 BP422_RS27015 WP_088910268.1 5673542..5674909(+) (radA) [Brevibacillus formosus strain NF2]
ATGTCAAAGTATAAAACGAAATACGCGTGTCAAGAATGTGGCTATGAATCGCCAAAATGGATGGGGAAATGCCCGGGTTG
CAGCAGTTGGAACACCCTCGTAGAGGAAGTGACGGTAAAAAATGCACATCGTCACGAAGGACTGAGTGGTGGCCAGCGCC
AATCTGCGATTCCGTTGACCCAGGTAGCCAGTGAAGAAGAGCCACGCATGGATACGACGATCGGAGAATTGAATCGTGTT
CTTGGTGGAGGACTTGTTCCGGGTTCACTCATTTTGGTTGGGGGAGACCCCGGAATCGGGAAATCGACATTGCTTTTGCA
GACATCGTTTGCCTTGGCTCATCAAGGGGCGAAGGTTCTCTATGTATCGGGAGAGGAGTCGGCAAAGCAAATCAAAATTC
GCGCGGATCGACTCCATTTGCAAACGCCTCCGATGTTTGTATTGGCAGAAAATGATTTGGATTTGATCGAACAGCATATT
AATCAAGTAGACCCGGATGTATTGATTATTGACTCGATCCAGACCGTGTTTCACCCGACGATTCAATCGGCTGCGGGGAG
CGTCGCACAAGTGCGGGAGGCTACTGCCCAATTGATGCGGATTGCAAAAGGGAAAGGGATTGGTACCTTCATCGTCGGTC
ACGTAACCAAGGAAGGCTCCATCGCTGGTCCGCGTATGCTTGAGCACATGGTGGATGCTGTTCTCTATTTTGAAGGGGAG
CGCCACAATACGTTTCGAATTTTGCGCGCCGTCAAAAACCGTTTTGGTTCGACAAACGAAATCGGTATTTTCGAGATGAA
GGATCGCGGTTTGGAGGAAGTGGCGAATCCATCTGAGATATTTTTGGCAGAGCGCCCATTTGGTGTTGCGGGTTCCACAG
TCGTAGCCAGTATGGAGGGCACACGTCCCGTTTTGGTTGAGCTGCAAGCTCTCGTTGCCCCGACCAGCTTTGTAACCCCT
CGGAGAACGGCGACAGGGGTCGATCATCAGCGTGTGGCGATGATCATGGCAGTGTTGGAAAAGCGCATGGGCATGATGCT
GCAAAATCAGGATGCTTATGTAAACGTAGCAGGCGGGGTGAGATTGGATGAGCCAGCGGTTGATTTGGCGATTGCCGTAA
GCATTGCAAGCAGTTTCCGTGACCACGCCACCAATCCGCATGATGTCGTTATCGGTGAAATTGGCTTGACTGGTGAGGTG
CGCGGTGTCTCTCGTATTGAGCAGCGAGTACGAGAAGCACATAAATTAGGCTTTAAGCGTGTTATCATCCCGGAGAAAAA
CATCCGCGGTTTGGATGCGCCAGCAGATATTCAAGTAATTGGTGTGAGCAATATTGCCGATGCGTTGAACGAGGTGATAA
GGAGGTAG

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(71-219)

Subunit ChlI of Mg-chelatase (ChlI)

(329-427)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A220MP74

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

65.929

99.341

0.655
  radA Streptococcus pneumoniae Rx1

58.057

99.56

0.578
  radA Streptococcus pneumoniae D39

58.057

99.56

0.578
  radA Streptococcus pneumoniae R6

58.057

99.56

0.578
  radA Streptococcus pneumoniae TIGR4

58.057

99.56

0.578
  radA Streptococcus mitis NCTC 12261

58.057

99.56

0.578
  radA Streptococcus mitis SK321

58.057

99.56

0.578

Multiple sequence alignment