Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   CNQ53_RS01575 Genome accession   NZ_CP023364
Coordinates   144343..145011 (+) Length   222 a.a.
NCBI ID   WP_001297375.1    Uniprot ID   P65957
Organism   Escherichia coli strain 144     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 139343..150011
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CNQ53_RS01540 (CNQ53_01535) yicC 139362..140225 (-) 864 WP_000621336.1 YicC/YloC family endoribonuclease -
  CNQ53_RS01550 (CNQ53_01545) rph 140352..141068 (+) 717 WP_001247093.1 ribonuclease PH -
  CNQ53_RS01555 (CNQ53_01550) pyrE 141134..141775 (+) 642 WP_000806177.1 orotate phosphoribosyltransferase -
  CNQ53_RS01560 (CNQ53_01555) slmA 141812..142408 (-) 597 WP_000818601.1 nucleoid occlusion factor SlmA -
  CNQ53_RS01565 (CNQ53_01560) dut 142515..142973 (-) 459 WP_000976070.1 dUTP diphosphatase -
  CNQ53_RS01570 (CNQ53_01565) coaBC 142951..144171 (-) 1221 WP_000050171.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  CNQ53_RS01575 (CNQ53_01570) radC 144343..145011 (+) 669 WP_001297375.1 DNA repair protein RadC Machinery gene
  CNQ53_RS01580 (CNQ53_01575) rpmB 145228..145464 (+) 237 WP_000091955.1 50S ribosomal protein L28 -
  CNQ53_RS01585 (CNQ53_01580) rpmG 145485..145652 (+) 168 WP_001051798.1 50S ribosomal protein L33 -
  CNQ53_RS01590 (CNQ53_01585) mutM 145750..146559 (+) 810 WP_001114542.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  CNQ53_RS01595 (CNQ53_01590) coaD 146598..147077 (-) 480 WP_001171866.1 pantetheine-phosphate adenylyltransferase -
  CNQ53_RS01600 (CNQ53_01595) waaA 147085..148362 (-) 1278 WP_000891564.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  CNQ53_RS01605 (CNQ53_01600) rfaQ 148775..149833 (+) 1059 WP_001521608.1 lipopolysaccharide core heptosyltransferase RfaQ -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25258.35 Da        Isoelectric Point: 7.9748

>NTDB_id=206359 CNQ53_RS01575 WP_001297375.1 144343..145011(+) (radC) [Escherichia coli strain 144]
MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=206359 CNQ53_RS01575 WP_001297375.1 144343..145011(+) (radC) [Escherichia coli strain 144]
GTGAAAAACAATGCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACGGATGTCGAGCT
GCTGGCGCTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAACAATTTAGTGGTGTTCATGGAATTGGCGTGGCGAAATTTGCCCAGTTA
AAGGGGATTGCTGAACTAGCGCGGCGTTACTACAACGTGCGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GCGGGAATTTTTACAAAGCCAGCTCACGGGGGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCACAACACCGGG
TTATAACGCATAGCCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAATGCCTCGGCGCTGATCCTTGCACATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

RadC-like JAB domain (RadC)

(103-220)

UPF0758 N-terminal (UPF0758_N)

(9-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P65957

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496

Multiple sequence alignment