Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   BO996_RS03480 Genome accession   NZ_CP018101
Coordinates   759626..761242 (+) Length   538 a.a.
NCBI ID   WP_071954006.1    Uniprot ID   -
Organism   Delftia sp. HK171     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 754626..766242
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BO996_RS03455 (BO996_03405) argS 754813..756510 (+) 1698 WP_043825927.1 arginine--tRNA ligase -
  BO996_RS03460 (BO996_03410) - 756531..757220 (+) 690 WP_012202602.1 SPOR domain-containing protein -
  BO996_RS03465 (BO996_03415) - 757302..757952 (+) 651 WP_071954005.1 thiol:disulfide interchange protein DsbA/DsbL -
  BO996_RS03470 (BO996_03420) lptA 758124..758786 (+) 663 WP_013804753.1 lipopolysaccharide transport periplasmic protein LptA -
  BO996_RS03475 (BO996_03425) lptB 758783..759571 (+) 789 WP_012202605.1 LPS export ABC transporter ATP-binding protein -
  BO996_RS03480 (BO996_03430) rpoN/rpoN1 759626..761242 (+) 1617 WP_071954006.1 RNA polymerase factor sigma-54 Machinery gene
  BO996_RS03485 (BO996_03435) rtcR 761281..762888 (-) 1608 WP_071954007.1 RNA repair transcriptional activator RtcR -
  BO996_RS03495 (BO996_03445) - 763586..764725 (+) 1140 WP_012202608.1 slipin family protein -
  BO996_RS03500 (BO996_03450) - 764782..765279 (+) 498 WP_043820033.1 hypothetical protein -

Sequence

Protein


Download         Length: 538 a.a.        Molecular weight: 59291.37 Da        Isoelectric Point: 4.6422

>NTDB_id=205798 BO996_RS03480 WP_071954006.1 759626..761242(+) (rpoN/rpoN1) [Delftia sp. HK171]
MKPGLSLRVSQHLSLTPQLQQSIQLLQLSTLELAQEVEQMLGDNPFLELANEEGDHDAAAGTREPAAPGAEATESFQESR
ADQPSAAVEWEPSSDASSGDAVETSGTAESLSWEGDGQTNSGNDDRDWGSDAPSSRTRGQGEEDEADATELARSAESLTD
HLHRQALSLRLGEVDRAALRFLIENLNDDGYLEDPLDELAQGLAGDDLEECEELVHRFTVALRLLQSLEPVGVGARDLAE
CLTLQLRDQLADAEREGNGSAEDEDRQAVLRTALDICAQPMDLLARRDLRKLASLCGAREERLREALVLITRLEPRPGRR
FADVQRQIVVPDVIVRPVRSGAGRMDFSVQLNPDVMPKLRVHDIYASALRSHRGSEGHAALQQRLQEARWFIKNIQQRFD
TILRVSQAIVERQKNFFVHGELAMRPLVLRDIADALGLHESTISRVTTAKYMATPQGTYELKYFFGSGLGTEAGGNASST
AVRALIKQFVAAEDPKKPLSDNQLADMLKEQGIECARRTVAKYRELLKIPPTTLRKAL

Nucleotide


Download         Length: 1617 bp        

>NTDB_id=205798 BO996_RS03480 WP_071954006.1 759626..761242(+) (rpoN/rpoN1) [Delftia sp. HK171]
TTGAAGCCCGGTCTGTCGCTGCGCGTCTCCCAGCATCTTTCGCTCACTCCCCAGCTGCAGCAGTCGATACAGCTGCTGCA
GCTGTCCACGCTGGAGCTGGCGCAGGAAGTGGAGCAGATGCTGGGCGACAACCCCTTCCTGGAGCTGGCGAACGAGGAGG
GCGATCACGATGCGGCCGCTGGCACCCGCGAGCCTGCCGCGCCCGGCGCCGAGGCCACGGAGTCCTTCCAGGAAAGCCGC
GCAGACCAGCCTTCGGCGGCGGTGGAGTGGGAGCCCTCGTCGGACGCATCCTCCGGCGATGCCGTGGAGACTTCCGGTAC
GGCCGAGTCCCTGAGCTGGGAAGGCGACGGCCAGACCAACTCGGGCAACGATGACCGCGACTGGGGCAGCGATGCGCCCT
CCAGCCGCACGCGCGGGCAGGGCGAGGAAGACGAGGCCGATGCCACCGAACTGGCACGCAGCGCCGAAAGCCTGACCGAC
CACCTGCACCGCCAGGCCCTGAGCCTGCGCCTGGGCGAGGTGGACCGCGCCGCACTGCGCTTTCTGATCGAGAACCTCAA
CGACGACGGCTACCTGGAAGACCCGCTCGACGAGCTGGCACAGGGCCTGGCGGGCGACGATCTGGAGGAGTGCGAGGAAC
TGGTGCACCGCTTCACCGTGGCGCTGCGCCTGCTGCAAAGCCTGGAGCCCGTGGGCGTGGGCGCGCGCGACCTGGCCGAA
TGCCTGACGCTGCAACTGCGCGACCAGCTGGCCGATGCCGAGCGCGAAGGCAACGGCAGTGCCGAGGACGAAGACCGGCA
GGCCGTGCTGCGCACCGCGCTGGACATCTGCGCCCAGCCCATGGACCTGCTGGCGCGGCGCGACCTGCGCAAGCTCGCCA
GCCTGTGCGGTGCGCGCGAGGAGCGGCTGCGCGAGGCGCTGGTGCTGATCACCCGGCTGGAGCCACGCCCGGGCCGGCGC
TTTGCTGACGTGCAGCGCCAGATCGTCGTGCCCGACGTCATCGTGCGCCCCGTGCGCAGCGGCGCGGGCCGCATGGACTT
CAGCGTGCAGCTCAACCCGGACGTCATGCCCAAGCTGCGCGTGCACGACATCTATGCCAGCGCGCTGCGCAGCCACAGGG
GCAGCGAAGGCCATGCAGCGCTGCAGCAGCGGCTGCAGGAGGCGCGCTGGTTCATCAAGAACATCCAGCAGCGCTTCGAC
ACCATCCTGCGCGTGTCGCAGGCCATCGTGGAGCGCCAGAAGAACTTCTTCGTGCATGGCGAGCTGGCCATGCGCCCGCT
GGTGCTGCGCGACATTGCCGATGCGCTGGGCCTGCATGAATCCACCATCAGCCGCGTGACCACGGCCAAGTACATGGCCA
CGCCGCAGGGCACCTACGAGCTCAAGTACTTCTTCGGCTCGGGCCTGGGCACCGAGGCCGGTGGCAATGCCTCCAGCACG
GCCGTGCGCGCGCTGATCAAGCAGTTCGTCGCGGCCGAAGACCCCAAGAAGCCGCTGTCGGACAACCAGCTCGCCGACAT
GCTCAAGGAGCAGGGCATCGAATGCGCGCGGCGCACGGTGGCCAAGTACCGCGAGCTGCTCAAGATCCCGCCGACCACGC
TGCGCAAGGCGCTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-49)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(149-365)

Sigma-54, DNA binding domain (Sigma54_DBD)

(380-536)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

51.296

100

0.515

Multiple sequence alignment