Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   BRL64_RS11750 Genome accession   NZ_CP018100
Coordinates   2262659..2263729 (-) Length   356 a.a.
NCBI ID   WP_041117979.1    Uniprot ID   -
Organism   Bacillus safensis strain BRM1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2257659..2268729
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BRL64_RS11700 (BRL64_11495) sipW 2257753..2258325 (-) 573 WP_034280218.1 signal peptidase I SipW -
  BRL64_RS11705 (BRL64_11500) tapA 2258378..2258905 (-) 528 WP_081124569.1 amyloid fiber anchoring/assembly protein TapA -
  BRL64_RS11710 (BRL64_11505) - 2259205..2259483 (+) 279 WP_061109527.1 DUF3889 domain-containing protein -
  BRL64_RS11715 (BRL64_11510) - 2259518..2259712 (-) 195 WP_024422974.1 YqzE family protein -
  BRL64_RS11720 (BRL64_11515) - 2259772..2260155 (-) 384 WP_081124570.1 competence type IV pilus minor pilin ComGG -
  BRL64_RS11725 (BRL64_11520) comGF 2260152..2260679 (-) 528 WP_257788741.1 competence type IV pilus minor pilin ComGF -
  BRL64_RS19875 (BRL64_11525) comGE 2260591..2260905 (-) 315 WP_041106865.1 competence type IV pilus minor pilin ComGE -
  BRL64_RS11735 (BRL64_11530) comGD 2260889..2261335 (-) 447 WP_081124572.1 competence type IV pilus minor pilin ComGD -
  BRL64_RS11740 (BRL64_11535) comGC 2261328..2261621 (-) 294 WP_024422977.1 competence type IV pilus major pilin ComGC Machinery gene
  BRL64_RS11745 (BRL64_11540) comGB 2261638..2262678 (-) 1041 WP_081124573.1 competence type IV pilus assembly protein ComGB -
  BRL64_RS11750 (BRL64_11545) comGA 2262659..2263729 (-) 1071 WP_041117979.1 competence type IV pilus ATPase ComGA Machinery gene
  BRL64_RS11755 (BRL64_11550) - 2263907..2264782 (-) 876 WP_024422979.1 STAS domain-containing protein -
  BRL64_RS11765 (BRL64_11560) - 2265006..2265386 (-) 381 WP_081124574.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  BRL64_RS11770 (BRL64_11565) - 2265599..2265841 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  BRL64_RS11775 (BRL64_11570) - 2265887..2266525 (-) 639 WP_034280195.1 MBL fold metallo-hydrolase -
  BRL64_RS11780 (BRL64_11575) - 2266683..2266856 (+) 174 WP_003217333.1 DUF2759 domain-containing protein -
  BRL64_RS11785 (BRL64_11580) - 2266880..2267194 (-) 315 WP_024427984.1 MTH1187 family thiamine-binding protein -
  BRL64_RS11790 (BRL64_11585) - 2267271..2268524 (-) 1254 WP_081124575.1 MFS transporter -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40398.79 Da        Isoelectric Point: 9.4061

>NTDB_id=205771 BRL64_RS11750 WP_041117979.1 2262659..2263729(-) (comGA) [Bacillus safensis strain BRM1]
MYGIEYLRQELLEEACRMRASDVHIVPKEKEASVSFRVDSDLIQQRTIDKKSGERLIAHFKFLSSMDIGEKRRPQNGSLA
VMLRNGQVFIRMSTLPTVNDESLVIRILPQDHVPKIKHLSLFPKASARLLSFLNHSHGLILFTGPTNSGKTTTLYSLIQF
AKKNFNRNIITLEDPVETRNEEVLQVQVNEKAGITYAAGLRAILRHDPDVIVLGEIRDAETARTAIRAALTGHLVLSTLH
AKNAKGALYRMLEFGVTMNELEQTMVAIAAQRLIELTCPFCGERCQLYCKLNRPIRRTNVFELLFGKELGECIKEAKGEY
AHSSYETLQRLIRKGVALGYLSKNTYHRWVYEEASL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=205771 BRL64_RS11750 WP_041117979.1 2262659..2263729(-) (comGA) [Bacillus safensis strain BRM1]
TTGTATGGGATTGAATATTTAAGGCAAGAGCTGCTAGAAGAAGCATGCCGCATGAGGGCTTCTGATGTGCATATTGTGCC
AAAAGAAAAGGAAGCTTCGGTGTCATTTCGTGTTGATTCAGATTTAATCCAGCAGCGAACGATTGATAAAAAAAGCGGAG
AGAGGCTTATTGCACATTTTAAGTTTTTATCTTCTATGGATATCGGGGAAAAAAGGAGGCCGCAAAATGGCTCGCTAGCA
GTAATGCTAAGAAACGGTCAAGTGTTTATTCGGATGTCTACCTTGCCGACAGTGAATGATGAGAGCTTAGTGATTAGAAT
TTTACCGCAGGATCATGTTCCGAAAATAAAACATCTATCATTATTTCCGAAAGCCTCAGCCAGATTATTATCCTTTTTAA
ACCACTCGCATGGACTCATTTTATTTACTGGGCCAACAAATTCAGGGAAAACAACAACACTATATTCATTGATCCAGTTT
GCAAAAAAGAATTTTAACCGAAATATTATTACACTTGAAGATCCTGTCGAAACAAGAAATGAAGAAGTGCTGCAGGTTCA
AGTGAATGAAAAAGCCGGCATCACGTATGCCGCTGGATTACGCGCTATTTTAAGACATGATCCAGATGTGATTGTGCTAG
GAGAAATAAGAGATGCTGAAACGGCTAGAACGGCGATTAGAGCAGCACTGACAGGCCATTTAGTATTGAGTACGCTCCAT
GCAAAAAATGCAAAAGGAGCCCTTTACCGCATGCTTGAATTTGGTGTGACGATGAATGAACTTGAACAAACCATGGTTGC
GATTGCTGCCCAGCGATTAATCGAGCTCACCTGTCCTTTTTGCGGAGAAAGATGTCAGCTTTACTGTAAATTAAATCGAC
CGATCAGACGAACAAATGTATTTGAGCTGCTGTTCGGAAAAGAGCTTGGTGAGTGTATCAAGGAGGCTAAAGGAGAATAT
GCTCACTCGTCATATGAAACACTGCAAAGATTAATTCGTAAAGGAGTGGCACTCGGCTATTTATCAAAAAACACTTACCA
TCGCTGGGTTTATGAAGAAGCGAGCCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

64.689

99.438

0.643

Multiple sequence alignment