Detailed information
Bioinformatically predicted
Overview
| Name | nucA/comI | Type | Machinery gene |
| Locus tag | BRL64_RS09145 | Genome accession | NZ_CP018100 |
| Coordinates | 1774975..1775355 (+) | Length | 126 a.a. |
| NCBI ID | WP_044335941.1 | Uniprot ID | A0AAP7UKB4 |
| Organism | Bacillus safensis strain BRM1 | ||
| Function | cleavage of dsDNA into ssDNA (predicted from homology) DNA processing |
||
Related MGE
Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.
Gene-MGE association summary
| MGE type | MGE coordinates | Gene coordinates | Relative position | Distance (bp) |
|---|---|---|---|---|
| Prophage | 1718009..1779471 | 1774975..1775355 | within | 0 |
Gene organization within MGE regions
Location: 1718009..1779471
| Locus tag | Gene name | Coordinates (strand) | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| BRL64_RS08830 (BRL64_08675) | - | 1718009..1718758 (+) | 750 | WP_081124415.1 | poly-gamma-glutamate hydrolase family protein | - |
| BRL64_RS08835 (BRL64_08680) | - | 1718921..1720069 (+) | 1149 | WP_076839615.1 | Rap family tetratricopeptide repeat protein | - |
| BRL64_RS08840 (BRL64_08685) | - | 1720159..1721484 (-) | 1326 | WP_081124416.1 | S8 family peptidase | - |
| BRL64_RS08845 (BRL64_08690) | - | 1722069..1722803 (+) | 735 | WP_024427035.1 | poly-gamma-glutamate hydrolase family protein | - |
| BRL64_RS08850 (BRL64_08695) | - | 1722880..1723329 (+) | 450 | WP_034281924.1 | OsmC family protein | - |
| BRL64_RS08855 (BRL64_08700) | - | 1723377..1723745 (-) | 369 | WP_034281925.1 | DMT family transporter | - |
| BRL64_RS08860 (BRL64_08705) | - | 1723761..1724078 (-) | 318 | WP_034281927.1 | DMT family transporter | - |
| BRL64_RS08865 (BRL64_08710) | - | 1724081..1724623 (-) | 543 | WP_081124417.1 | TetR/AcrR family transcriptional regulator | - |
| BRL64_RS08870 (BRL64_08715) | - | 1724736..1724999 (-) | 264 | WP_025093279.1 | hypothetical protein | - |
| BRL64_RS08875 (BRL64_08720) | - | 1725104..1725505 (+) | 402 | WP_034281930.1 | YmaF family protein | - |
| BRL64_RS08880 (BRL64_08725) | miaA | 1725576..1726532 (+) | 957 | WP_034281931.1 | tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA | - |
| BRL64_RS08885 (BRL64_08730) | hfq | 1726558..1726779 (+) | 222 | WP_024424088.1 | RNA chaperone Hfq | - |
| BRL64_RS08890 (BRL64_08735) | - | 1726942..1727262 (+) | 321 | WP_081124418.1 | YmzC family protein | - |
| BRL64_RS08895 (BRL64_08740) | - | 1727342..1727554 (+) | 213 | WP_034281933.1 | hypothetical protein | - |
| BRL64_RS08900 (BRL64_08745) | nrdI | 1727820..1728212 (+) | 393 | WP_008360380.1 | class Ib ribonucleoside-diphosphate reductase assembly flavoprotein NrdI | - |
| BRL64_RS08905 (BRL64_08750) | nrdE | 1728172..1730274 (+) | 2103 | WP_034281935.1 | class 1b ribonucleoside-diphosphate reductase subunit alpha | - |
| BRL64_RS08910 (BRL64_08755) | nrdF | 1730292..1731272 (+) | 981 | WP_024424085.1 | class 1b ribonucleoside-diphosphate reductase subunit beta | - |
| BRL64_RS08915 (BRL64_08760) | - | 1731357..1731974 (+) | 618 | WP_024424084.1 | hypothetical protein | - |
| BRL64_RS08920 (BRL64_08765) | - | 1732042..1732800 (-) | 759 | WP_081124419.1 | N-acetylmuramoyl-L-alanine amidase | - |
| BRL64_RS08925 (BRL64_08770) | spoVK | 1733121..1734080 (+) | 960 | WP_024424083.1 | stage V sporulation protein K | - |
| BRL64_RS08930 (BRL64_08775) | hflX | 1734202..1735473 (+) | 1272 | WP_034623554.1 | GTPase HflX | - |
| BRL64_RS08935 (BRL64_08780) | - | 1735480..1736760 (+) | 1281 | WP_081124420.1 | aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme | - |
| BRL64_RS08940 (BRL64_08785) | - | 1736852..1737262 (+) | 411 | WP_034281940.1 | MerR family transcriptional regulator | - |
| BRL64_RS08945 (BRL64_08790) | glnA | 1737323..1738657 (+) | 1335 | WP_025093273.1 | type I glutamate--ammonia ligase | - |
| BRL64_RS08950 (BRL64_08795) | - | 1738776..1738988 (-) | 213 | Protein_1705 | tyrosine-type recombinase/integrase | - |
| BRL64_RS08955 (BRL64_08800) | - | 1739005..1739169 (+) | 165 | WP_231293998.1 | XtrA/YqaO family protein | - |
| BRL64_RS08960 (BRL64_08805) | - | 1739327..1740394 (+) | 1068 | WP_081124423.1 | patatin-like phospholipase family protein | - |
| BRL64_RS19735 | - | 1740556..1740830 (+) | 275 | Protein_1708 | DNA-methyltransferase | - |
| BRL64_RS08970 (BRL64_08810) | - | 1741002..1741694 (+) | 693 | WP_046311156.1 | hypothetical protein | - |
| BRL64_RS08975 (BRL64_08815) | - | 1741921..1742289 (+) | 369 | WP_155889685.1 | hypothetical protein | - |
| BRL64_RS19970 | - | 1742253..1742402 (+) | 150 | WP_155889686.1 | DUF134 domain-containing protein | - |
| BRL64_RS08980 (BRL64_08820) | - | 1742406..1742860 (+) | 455 | Protein_1712 | sigma-70 family RNA polymerase sigma factor | - |
| BRL64_RS08985 (BRL64_08825) | - | 1743017..1743493 (+) | 477 | WP_081124425.1 | hypothetical protein | - |
| BRL64_RS08990 (BRL64_08830) | - | 1743573..1743779 (+) | 207 | WP_081124426.1 | hypothetical protein | - |
| BRL64_RS08995 (BRL64_08835) | - | 1743950..1744391 (+) | 442 | Protein_1715 | terminase gpP N-terminus-related DNA-binding protein | - |
| BRL64_RS09000 (BRL64_08840) | - | 1744522..1745450 (+) | 929 | Protein_1716 | phage minor head protein | - |
| BRL64_RS09005 (BRL64_08845) | - | 1745471..1746346 (+) | 876 | WP_081124427.1 | hypothetical protein | - |
| BRL64_RS09010 (BRL64_08850) | - | 1746348..1746748 (+) | 401 | Protein_1718 | XkdF-like putative serine protease domain-containing protein | - |
| BRL64_RS09015 (BRL64_08855) | - | 1747252..1748385 (+) | 1134 | WP_046311164.1 | RapH N-terminal domain-containing protein | - |
| BRL64_RS09020 (BRL64_08860) | - | 1748765..1750690 (+) | 1926 | WP_081124428.1 | T7SS effector LXG polymorphic toxin | - |
| BRL64_RS09025 (BRL64_08865) | - | 1750695..1751177 (+) | 483 | WP_196797912.1 | immunity protein YezG family protein | - |
| BRL64_RS09030 (BRL64_08870) | - | 1751211..1751669 (-) | 459 | WP_081124429.1 | macro domain-containing protein | - |
| BRL64_RS09035 (BRL64_08875) | - | 1751872..1752413 (+) | 542 | Protein_1723 | GNAT family N-acetyltransferase | - |
| BRL64_RS09040 (BRL64_08880) | - | 1752556..1753137 (+) | 582 | WP_081124430.1 | undecaprenyl-diphosphatase | - |
| BRL64_RS09045 (BRL64_08885) | - | 1753190..1753561 (-) | 372 | WP_034621969.1 | iron chaperone | - |
| BRL64_RS09050 (BRL64_08890) | - | 1754105..1755217 (+) | 1113 | WP_081124431.1 | tetratricopeptide repeat protein | - |
| BRL64_RS09060 (BRL64_08895) | - | 1755942..1756637 (-) | 696 | WP_046311182.1 | YoaK family protein | - |
| BRL64_RS09065 (BRL64_08900) | - | 1756705..1758537 (-) | 1833 | WP_081124432.1 | DNA ligase D | - |
| BRL64_RS09070 (BRL64_08905) | - | 1758653..1759501 (+) | 849 | WP_081124433.1 | Ku protein | - |
| BRL64_RS09075 (BRL64_08910) | - | 1759719..1760162 (-) | 444 | WP_081124434.1 | DUF2188 domain-containing protein | - |
| BRL64_RS09080 (BRL64_08915) | - | 1760323..1760796 (+) | 474 | WP_081124435.1 | VOC family protein | - |
| BRL64_RS09085 (BRL64_08920) | - | 1760845..1761258 (+) | 414 | WP_034283385.1 | Rrf2 family transcriptional regulator | - |
| BRL64_RS09090 (BRL64_08925) | - | 1761358..1762209 (+) | 852 | WP_081124436.1 | SDR family oxidoreductase | - |
| BRL64_RS09095 (BRL64_08930) | - | 1762400..1763122 (+) | 723 | WP_081124437.1 | (Fe-S)-binding protein | - |
| BRL64_RS09100 (BRL64_08935) | - | 1763138..1764559 (+) | 1422 | WP_081124438.1 | LutB/LldF family L-lactate oxidation iron-sulfur protein | - |
| BRL64_RS09105 (BRL64_08940) | - | 1764559..1765281 (+) | 723 | WP_081124439.1 | LutC/YkgG family protein | - |
| BRL64_RS09110 (BRL64_08945) | - | 1765427..1766335 (+) | 909 | WP_034283398.1 | DMT family transporter | - |
| BRL64_RS09115 (BRL64_08950) | - | 1766349..1766540 (-) | 192 | WP_081124440.1 | hypothetical protein | - |
| BRL64_RS09120 (BRL64_08955) | - | 1766651..1768867 (+) | 2217 | WP_081124441.1 | RNA ligase | - |
| BRL64_RS09125 (BRL64_08960) | - | 1768932..1769642 (+) | 711 | WP_081124442.1 | DUF421 domain-containing protein | - |
| BRL64_RS09130 (BRL64_08965) | - | 1769871..1770449 (+) | 579 | WP_034283407.1 | TetR/AcrR family transcriptional regulator | - |
| BRL64_RS09135 (BRL64_08970) | - | 1770471..1773614 (+) | 3144 | WP_081124443.1 | bifunctional cytochrome P450/NADPH--P450 reductase | - |
| BRL64_RS09140 (BRL64_08975) | - | 1773808..1774716 (+) | 909 | WP_024425124.1 | trypsin-like serine peptidase | - |
| BRL64_RS09145 (BRL64_08980) | nucA/comI | 1774975..1775355 (+) | 381 | WP_044335941.1 | NucA/NucB deoxyribonuclease domain-containing protein | Machinery gene |
| BRL64_RS09150 (BRL64_08985) | - | 1775501..1776349 (+) | 849 | WP_041117519.1 | STAS domain-containing protein | - |
| BRL64_RS09155 (BRL64_08990) | - | 1776382..1776924 (-) | 543 | WP_041109224.1 | IseA DL-endopeptidase inhibitor family protein | - |
| BRL64_RS09160 (BRL64_08995) | - | 1777221..1777670 (+) | 450 | WP_041109226.1 | hypothetical protein | - |
| BRL64_RS09165 (BRL64_09000) | lexA | 1777716..1778336 (-) | 621 | WP_034283422.1 | transcriptional repressor LexA | - |
| BRL64_RS09170 (BRL64_09005) | yneA | 1778495..1778806 (+) | 312 | WP_024425130.1 | cell division suppressor protein YneA | - |
| BRL64_RS09175 (BRL64_09010) | - | 1778824..1779471 (+) | 648 | WP_034283427.1 | YneB family resolvase-like protein | - |
Sequence
Protein
Download Length: 126 a.a. Molecular weight: 13727.41 Da Isoelectric Point: 8.5068
>NTDB_id=205765 BRL64_RS09145 WP_044335941.1 1774975..1775355(+) (nucA/comI) [Bacillus safensis strain BRM1]
MGTLLGGFGEKQAAKGADRYDHVVQFPKERYPETGSHIQEAIRKGHSDVCTIDRNGADARRQESLKGIPTKPGFDRDEWP
MAVCLEGGKGASVQYVSPSDNRGAGSWVGHQISGFPDGKRILFIVK
MGTLLGGFGEKQAAKGADRYDHVVQFPKERYPETGSHIQEAIRKGHSDVCTIDRNGADARRQESLKGIPTKPGFDRDEWP
MAVCLEGGKGASVQYVSPSDNRGAGSWVGHQISGFPDGKRILFIVK
Nucleotide
Download Length: 381 bp
>NTDB_id=205765 BRL64_RS09145 WP_044335941.1 1774975..1775355(+) (nucA/comI) [Bacillus safensis strain BRM1]
ATGGGCACGTTGTTAGGTGGATTTGGAGAGAAGCAGGCAGCAAAAGGGGCTGATCGATATGATCATGTCGTTCAATTTCC
AAAGGAAAGGTACCCTGAAACAGGCAGTCATATTCAAGAAGCCATTCGAAAAGGGCATTCAGATGTGTGTACCATTGACC
GAAATGGAGCAGATGCCCGCAGGCAAGAATCATTAAAAGGAATTCCGACAAAACCTGGCTTTGACCGGGATGAATGGCCA
ATGGCGGTTTGTCTTGAGGGAGGAAAAGGCGCAAGCGTACAATATGTTAGTCCATCTGATAATAGGGGAGCAGGCTCATG
GGTCGGGCACCAAATCAGCGGGTTTCCTGATGGGAAAAGAATATTATTCATTGTGAAATAA
ATGGGCACGTTGTTAGGTGGATTTGGAGAGAAGCAGGCAGCAAAAGGGGCTGATCGATATGATCATGTCGTTCAATTTCC
AAAGGAAAGGTACCCTGAAACAGGCAGTCATATTCAAGAAGCCATTCGAAAAGGGCATTCAGATGTGTGTACCATTGACC
GAAATGGAGCAGATGCCCGCAGGCAAGAATCATTAAAAGGAATTCCGACAAAACCTGGCTTTGACCGGGATGAATGGCCA
ATGGCGGTTTGTCTTGAGGGAGGAAAAGGCGCAAGCGTACAATATGTTAGTCCATCTGATAATAGGGGAGCAGGCTCATG
GGTCGGGCACCAAATCAGCGGGTTTCCTGATGGGAAAAGAATATTATTCATTGTGAAATAA
3D structure
| Source | ID | Structure |
|---|
Similar proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| nucA/comI | Bacillus subtilis subsp. subtilis str. 168 |
57.983 |
94.444 |
0.548 |