Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   BRL64_RS01975 Genome accession   NZ_CP018100
Coordinates   357932..358360 (-) Length   142 a.a.
NCBI ID   WP_024424305.1    Uniprot ID   A0A5C0WGQ8
Organism   Bacillus safensis strain BRM1     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 352932..363360
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BRL64_RS01945 (BRL64_01920) - 353077..353406 (+) 330 WP_024424311.1 YckD family protein -
  BRL64_RS01950 (BRL64_01925) - 353450..354214 (-) 765 WP_081123964.1 MBL fold metallo-hydrolase -
  BRL64_RS01955 (BRL64_01930) - 354330..354746 (+) 417 WP_180951418.1 Lrp/AsnC family transcriptional regulator -
  BRL64_RS01960 (BRL64_01935) - 354775..355581 (+) 807 WP_081123965.1 aminoglycoside N(3)-acetyltransferase -
  BRL64_RS01965 (BRL64_01940) - 355600..355986 (-) 387 WP_081123966.1 competence protein ComJ -
  BRL64_RS01970 (BRL64_01945) - 356107..357804 (-) 1698 WP_046313811.1 methyl-accepting chemotaxis protein -
  BRL64_RS01975 (BRL64_01950) nucA/comI 357932..358360 (-) 429 WP_024424305.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  BRL64_RS01980 (BRL64_01955) - 358728..360797 (+) 2070 WP_081123967.1 hydantoinase/oxoprolinase family protein -
  BRL64_RS01985 (BRL64_01960) - 360787..362775 (+) 1989 WP_081123968.1 hydantoinase B/oxoprolinase family protein -

Sequence

Protein


Download         Length: 142 a.a.        Molecular weight: 15971.96 Da        Isoelectric Point: 6.2215

>NTDB_id=205741 BRL64_RS01975 WP_024424305.1 357932..358360(-) (nucA/comI) [Bacillus safensis strain BRM1]
MKLLKMILLLLLIVIGVATGYIQLEQGKQETANSSYDKTIHFPSDRYPETAKHIEEAIDEGHSSICTIDRKHSDEQRDRS
LHGVPTKRGYDRDEWPMAMCKEGGTGASVKYVRPSDNRGAGSWVSHQLSDDPDGTRIQFIID

Nucleotide


Download         Length: 429 bp        

>NTDB_id=205741 BRL64_RS01975 WP_024424305.1 357932..358360(-) (nucA/comI) [Bacillus safensis strain BRM1]
ATGAAACTTCTTAAAATGATTCTCTTGCTTTTATTGATTGTTATTGGTGTAGCAACAGGCTATATCCAGCTGGAGCAAGG
GAAACAAGAGACAGCGAATTCATCTTATGACAAAACGATTCATTTTCCATCAGACCGCTATCCCGAAACAGCCAAGCACA
TAGAAGAAGCCATTGATGAAGGTCATTCGTCTATTTGTACAATTGACCGTAAACATAGTGATGAGCAGAGGGACCGATCA
TTACATGGCGTTCCAACAAAGCGCGGGTATGATCGTGACGAATGGCCAATGGCGATGTGCAAAGAAGGAGGCACAGGAGC
TTCTGTCAAATATGTACGCCCTTCTGATAATAGAGGCGCAGGCTCGTGGGTAAGTCATCAATTATCGGATGATCCAGACG
GCACAAGAATCCAATTTATCATCGATTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(46-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5C0WGQ8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

66.667

80.282

0.535

Multiple sequence alignment