Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   BRL64_RS00615 Genome accession   NZ_CP018100
Coordinates   109609..110988 (+) Length   459 a.a.
NCBI ID   WP_025092231.1    Uniprot ID   A0AAP7QRW5
Organism   Bacillus safensis strain BRM1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 104609..115988
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BRL64_RS00595 (BRL64_00595) - 104951..105415 (+) 465 WP_003217184.1 CtsR family transcriptional regulator -
  BRL64_RS00600 (BRL64_00600) - 105430..105987 (+) 558 WP_024425862.1 UvrB/UvrC motif-containing protein -
  BRL64_RS00605 (BRL64_00605) - 105992..107083 (+) 1092 WP_024425579.1 protein arginine kinase -
  BRL64_RS00610 (BRL64_00610) clpC 107080..109515 (+) 2436 WP_007496263.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  BRL64_RS00615 (BRL64_00615) radA 109609..110988 (+) 1380 WP_025092231.1 DNA repair protein RadA Machinery gene
  BRL64_RS00620 (BRL64_00620) disA 110991..112070 (+) 1080 WP_024425581.1 DNA integrity scanning diadenylate cyclase DisA -
  BRL64_RS00625 (BRL64_00625) - 112224..113324 (+) 1101 WP_024425582.1 PIN/TRAM domain-containing protein -
  BRL64_RS00630 (BRL64_00630) ispD 113338..114027 (+) 690 WP_041115162.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  BRL64_RS00635 (BRL64_00635) ispF 114031..114507 (+) 477 WP_003216928.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 50009.49 Da        Isoelectric Point: 7.3829

>NTDB_id=205740 BRL64_RS00615 WP_025092231.1 109609..110988(+) (radA) [Bacillus safensis strain BRM1]
MAKTKSKFICQSCGYESAKWMGKCPGCGTWNSMTEEVVRKEPANRRSAFNHSVQTIQKPSPISAIETSEEPRIKTNLEEF
NRVLGSGIVKGSLVLIGGDPGIGKSTLLLQVSAQLSDKNQNVLYISGEESIKQTKLRADRLGIESASLHVLAETDMEYIT
SAIQEMKPAFVVVDSIQTVYQSDITSAPGSVSQVRECTAQLMKIAKTNGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNELGIFEMREEGLTEVLNPSEIFLEERSAGVSGSCVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGLDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAIAVSIASSFKDAAPHQADCFIGEVGLT
GEVRRVSRIEQRVQEAAKLGFKRMFIPQANIDGWKKPRGIELVGVENVAEALRISLGGS

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=205740 BRL64_RS00615 WP_025092231.1 109609..110988(+) (radA) [Bacillus safensis strain BRM1]
ATGGCTAAGACAAAATCAAAATTTATATGCCAATCGTGCGGTTATGAGTCAGCCAAATGGATGGGGAAATGTCCAGGCTG
CGGCACGTGGAACAGTATGACAGAAGAGGTCGTTCGCAAAGAGCCGGCAAACCGTCGAAGTGCTTTTAATCATTCTGTTC
AAACCATTCAAAAACCTTCACCTATATCTGCAATTGAAACATCCGAAGAACCCCGAATTAAAACAAATTTAGAAGAATTT
AACCGAGTATTAGGAAGTGGAATTGTCAAAGGTTCTCTTGTTCTCATCGGCGGAGATCCTGGGATTGGGAAGTCCACATT
ATTATTACAAGTATCAGCACAGCTCTCAGACAAAAATCAGAATGTGTTATACATATCTGGTGAGGAGTCCATTAAACAAA
CGAAGCTAAGAGCGGACCGCCTTGGCATTGAAAGCGCCTCTTTACATGTTTTGGCTGAAACCGATATGGAGTATATAACG
TCTGCTATACAGGAGATGAAACCTGCTTTTGTTGTGGTGGATTCGATTCAAACCGTTTACCAAAGCGATATCACGTCAGC
TCCCGGCTCTGTATCTCAAGTTAGAGAATGTACAGCGCAGCTGATGAAGATTGCCAAGACAAATGGTATTCCTATTTTTA
TCGTTGGTCACGTGACCAAAGAAGGCTCGATCGCAGGTCCACGCCTTTTAGAGCATATGGTGGACACAGTTCTTTATTTT
GAAGGCGAGCGTCATCATACGTTTCGTATCTTACGGGCGGTCAAAAACCGATTTGGTTCAACGAATGAATTGGGAATTTT
TGAAATGAGAGAGGAAGGGCTCACGGAAGTATTAAACCCATCAGAAATCTTTCTAGAAGAGCGGTCGGCAGGTGTATCTG
GTTCGTGTGTTGTTGCCTCAATGGAAGGAACAAGACCTGTACTTGTCGAGATACAGGCATTAATTTCCCCGACAAGCTTT
GGGAATCCGCGAAGAATGGCCACTGGCCTTGATCATAATCGAGTATCGCTGCTCATGGCAGTTTTAGAAAAACGCGTGGG
GCTGCTGCTGCAAAACCAAGATGCGTATTTAAAGGTCGCAGGCGGTGTGAAGCTTGACGAACCGGCGATTGACTTAGCTA
TTGCCGTCAGTATTGCATCAAGCTTTAAAGATGCAGCGCCGCATCAAGCGGATTGCTTTATAGGAGAGGTCGGTCTGACG
GGAGAAGTCAGAAGAGTATCAAGAATTGAACAGCGAGTGCAGGAAGCTGCAAAACTAGGATTTAAGCGAATGTTTATTCC
TCAGGCGAATATAGACGGATGGAAAAAGCCGAGAGGGATTGAGTTAGTTGGTGTAGAAAATGTAGCGGAGGCACTTCGAA
TTTCACTAGGGGGATCATAG

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(67-222)

Subunit ChlI of Mg-chelatase (ChlI)

(365-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

87.336

99.782

0.871
  radA Streptococcus mitis NCTC 12261

61.589

98.693

0.608
  radA Streptococcus pneumoniae Rx1

61.589

98.693

0.608
  radA Streptococcus pneumoniae D39

61.589

98.693

0.608
  radA Streptococcus pneumoniae R6

61.589

98.693

0.608
  radA Streptococcus pneumoniae TIGR4

61.589

98.693

0.608
  radA Streptococcus mitis SK321

61.589

98.693

0.608

Multiple sequence alignment