Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   Y71_RS02595 Genome accession   NZ_CP018016
Coordinates   527880..529313 (-) Length   477 a.a.
NCBI ID   WP_035887975.1    Uniprot ID   -
Organism   Kosakonia radicincitans DSM 16656     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 522880..534313
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Y71_RS02565 (Y71_02505) elbB 524448..525101 (+) 654 WP_007369891.1 isoprenoid biosynthesis glyoxalase ElbB -
  Y71_RS02570 (Y71_02510) mtgA 525098..525829 (+) 732 WP_035942012.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  Y71_RS02575 (Y71_02515) npr 525831..526103 (-) 273 WP_007369893.1 PTS phosphocarrier protein NPr -
  Y71_RS02580 (Y71_02520) rapZ 526100..526954 (-) 855 WP_007369894.1 RNase adapter RapZ -
  Y71_RS02585 (Y71_02525) ptsN 526997..527488 (-) 492 WP_007369895.1 PTS IIA-like nitrogen regulatory protein PtsN -
  Y71_RS02590 (Y71_02530) hpf 527570..527857 (-) 288 WP_007369896.1 ribosome hibernation promoting factor -
  Y71_RS02595 (Y71_02535) rpoN/rpoN1 527880..529313 (-) 1434 WP_035887975.1 RNA polymerase factor sigma-54 Machinery gene
  Y71_RS02600 (Y71_02540) lptB 529362..530087 (-) 726 WP_007369898.1 LPS export ABC transporter ATP-binding protein -
  Y71_RS02605 (Y71_02545) lptA 530094..530648 (-) 555 WP_007369899.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  Y71_RS02610 (Y71_02550) lptC 530617..531192 (-) 576 WP_007369900.1 LPS export ABC transporter periplasmic protein LptC -
  Y71_RS02615 (Y71_02555) kdsC 531189..531755 (-) 567 WP_007369901.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  Y71_RS02620 (Y71_02560) kdsD 531770..532756 (-) 987 WP_007369902.1 arabinose-5-phosphate isomerase KdsD -
  Y71_RS02625 (Y71_02565) - 532770..533744 (-) 975 WP_007369903.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53802.57 Da        Isoelectric Point: 4.3889

>NTDB_id=205046 Y71_RS02595 WP_035887975.1 527880..529313(-) (rpoN/rpoN1) [Kosakonia radicincitans DSM 16656]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQADLHEEVDSQQTQENEGLDTVDALEQKEMPE
ELPLDASWDEIYTAGTPSGSSHDYIDDELPIYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVSLED
VLESMGDEEIGLDEVEAVLKRVQRFDPVGVAARDLRDCLLIQLSQFAKDTPWCEEARLIISDHLDLLANHDFRSLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGRWTVELNSDSIPRLQINQHYASMGGGARNDADTQFIR
SNLQEAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEFMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTLLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=205046 Y71_RS02595 WP_035887975.1 527880..529313(-) (rpoN/rpoN1) [Kosakonia radicincitans DSM 16656]
ATGAAGCAAGGTTTGCAATTAAGGCTTAGCCAACAGCTAGCTATGACGCCGCAACTGCAACAGGCTATCCGTCTGTTGCA
GCTGTCTACGTTAGAACTTCAGCAGGAACTTCAGCAAGCGCTGGAGAGTAACCCATTGCTGGAACAAGCCGATCTTCATG
AAGAAGTGGATAGCCAACAAACCCAGGAAAATGAAGGCCTGGATACTGTAGACGCGCTCGAACAGAAAGAGATGCCTGAA
GAGCTGCCGCTGGACGCCAGCTGGGATGAGATTTATACCGCCGGAACGCCTTCCGGCAGCAGCCACGATTATATTGATGA
CGAACTGCCGATTTATCAGGGTGAAACGACACAGTCTCTTCAGGATTACCTGATGTGGCAGGTAGAGTTAACGCCGTTTT
CCGATACCGATCGCGCAATCGCAACCTCTATTGTCGATGCTGTCGATGAGACCGGCTACCTTACCGTGTCGCTTGAGGAT
GTGCTGGAAAGCATGGGCGATGAAGAAATCGGTCTCGATGAAGTTGAAGCGGTATTAAAGCGCGTTCAGCGTTTCGATCC
GGTTGGCGTCGCTGCGCGCGATCTGCGCGACTGCCTGTTGATCCAACTGTCCCAATTCGCGAAAGATACGCCCTGGTGTG
AAGAGGCTCGCCTGATTATCAGCGATCACCTTGATCTGCTCGCTAACCATGATTTCCGCTCTCTCATGCGCGTCACACGT
CTTAAAGAGGATGTGCTGAAAGAAGCAGTAAATCTGATCCAGTCGCTGGATCCGCGCCCCGGCCAGTCGATCCAGACCGG
TGAACCAGAATATGTCATCCCGGATGTGCTGGTGCGTAAGCACAACGGGCGCTGGACGGTAGAACTGAACTCCGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCTTCAATGGGCGGCGGCGCGCGCAATGACGCCGACACCCAGTTTATCCGC
AGTAATTTGCAGGAAGCGAAGTGGCTGATCAAAAGCCTTGAAAGCCGTAATGACACGCTATTGCGCGTCAGCCGCTGCAT
CGTCGAGCAGCAGCAGGCATTCTTTGAACAGGGCGAAGAGTTTATGAAACCGATGGTGCTGGCGGATATCGCTCAGGCCG
TCGAAATGCACGAGTCCACGATTTCCCGTGTGACAACGCAGAAGTACCTGCACAGTCCTCGCGGCATTTTTGAACTGAAG
TATTTCTTCTCCAGCCATGTGAATACGGAAGGCGGCGGCGAAGCCTCGTCAACAGCCATTCGCGCACTGGTTAAGAAATT
AATCGCCGCAGAAAACCCTGCAAAACCACTGAGCGACAGTAAGCTGACGACGCTCCTTTCCGATCAAGGAATTATGGTAG
CGCGGCGTACCGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCTAACCAGCGTAAACAGCTGGTGTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.747

100

0.482

Multiple sequence alignment