Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   BLL65_RS06960 Genome accession   NZ_CP018007
Coordinates   1345710..1346324 (+) Length   204 a.a.
NCBI ID   WP_032873955.1    Uniprot ID   -
Organism   Bacillus velezensis strain sx01604     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1340710..1351324
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BLL65_RS06925 (BLL65_06800) aroE 1341396..1342238 (+) 843 WP_032873946.1 shikimate dehydrogenase -
  BLL65_RS06930 (BLL65_06805) yhbY 1342232..1342522 (+) 291 WP_003152858.1 ribosome assembly RNA-binding protein YhbY -
  BLL65_RS06935 (BLL65_06810) - 1342533..1343102 (+) 570 WP_007408258.1 nicotinate-nucleotide adenylyltransferase -
  BLL65_RS06940 (BLL65_06815) yqeK 1343092..1343667 (+) 576 WP_032873949.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  BLL65_RS06945 (BLL65_06820) rsfS 1343671..1344027 (+) 357 WP_007408260.1 ribosome silencing factor -
  BLL65_RS06950 (BLL65_06825) - 1344024..1344761 (+) 738 WP_032873951.1 class I SAM-dependent DNA methyltransferase -
  BLL65_RS06955 (BLL65_06830) comER 1344830..1345651 (-) 822 WP_012118027.1 late competence protein ComER -
  BLL65_RS06960 (BLL65_06835) comEA 1345710..1346324 (+) 615 WP_032873955.1 helix-hairpin-helix domain-containing protein Machinery gene
  BLL65_RS06965 (BLL65_06840) - 1346391..1346960 (+) 570 WP_003152868.1 ComE operon protein 2 -
  BLL65_RS06970 (BLL65_06845) comEC 1346961..1349312 (+) 2352 WP_032873958.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  BLL65_RS06975 (BLL65_06850) - 1349331..1349465 (-) 135 WP_003152870.1 YqzM family protein -
  BLL65_RS19550 - 1349506..1349657 (+) 152 Protein_1372 hypothetical protein -
  BLL65_RS06980 (BLL65_06855) holA 1349697..1350738 (+) 1042 Protein_1373 DNA polymerase III subunit delta -
  BLL65_RS06985 (BLL65_06860) rpsT 1350755..1351021 (-) 267 WP_003152876.1 30S ribosomal protein S20 -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22265.47 Da        Isoelectric Point: 6.4940

>NTDB_id=204984 BLL65_RS06960 WP_032873955.1 1345710..1346324(+) (comEA) [Bacillus velezensis strain sx01604]
MTKAMMTERLRRNLKILGACLVAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDLKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREEKGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=204984 BLL65_RS06960 WP_032873955.1 1345710..1346324(+) (comEA) [Bacillus velezensis strain sx01604]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGTAGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGACAAGATTATGATTGATTTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACTCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAACGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAAAGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

58.115

93.627

0.544
  comEA Latilactobacillus sakei subsp. sakei 23K

31.897

100

0.363

Multiple sequence alignment