Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PSJM300_00765 Genome accession   CP003725
Coordinates   164793..166361 (+) Length   522 a.a.
NCBI ID   AFN76237.1    Uniprot ID   -
Organism   Pseudomonas stutzeri DSM 10701     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 159793..171361
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSJM300_00745 - 160254..161141 (+) 888 AFN76233.1 LysR family transcriptional regulator -
  PSJM300_00750 - 161203..162654 (-) 1452 AFN76234.1 NAD(P)(+) transhydrogenase -
  PSJM300_00755 - 162651..162971 (-) 321 AFN76235.1 NAD(P) transhydrogenase subunit alpha part 2 -
  PSJM300_00760 - 162985..164106 (-) 1122 AFN76236.1 NAD(P) transhydrogenase subunit alpha -
  PSJM300_00765 pilB 164793..166361 (+) 1569 AFN76237.1 general secretion pathway protein E Machinery gene
  PSJM300_00770 - 166503..167717 (+) 1215 AFN76238.1 general secretion pathway protein F -
  PSJM300_00775 - 167723..168157 (+) 435 AFN76239.1 general secretion pathway protein G -
  PSJM300_00780 - 168161..168739 (+) 579 AFN76240.1 general secretion pathway protein H -
  PSJM300_00785 - 168729..169130 (+) 402 AFN76241.1 general secretion pathway protein I -
  PSJM300_00790 - 169127..169840 (+) 714 AFN76242.1 general secretion pathway protein J -
  PSJM300_00795 - 169840..170820 (+) 981 AFN76243.1 general secretion pathway protein K -

Sequence

Protein


Download         Length: 522 a.a.        Molecular weight: 57836.02 Da        Isoelectric Point: 6.3847

>NTDB_id=20436 PSJM300_00765 AFN76237.1 164793..166361(+) (pilB) [Pseudomonas stutzeri DSM 10701]
MSSLEGTLTPARLPFAFARRHGVFLRDGESGLRLCAREGAPLTALQEAQRVAGGPLPMQWLTPEAFDEALGNAYQHDSSD
AMLMVEGLGKDMDLHSLADHIEKTEDLLEQEDDAPIIRLINALLGEAIRENASDIHVETFEKRLVIRFRVDGILREVVQP
KRELAALLVSRIKVMARLDIAEKRVPQDGRISLRVGGREVDIRVSTLPSANGERVVLRLLDKQAGRLTLQHLGMSERDRQ
RMEQAVKRPHGIILVTGPTGSGKTTTLYASLTTLNDRTRNILTVEDPIEYNLEGIGQTQVNTKVDMTFARGLRAILRQDP
DVVMVGEIRDQETADMAVQASLTGHLVLSTLHTNSAIGAVTRLVDMGVEPFLISSSLLGVLAQRLVRVLCNDCKRAYVAD
EAECALFGIDPSEAPTLYHAEGCDVCRQLGYRGRTGIYELVLFDDSLRSMIHTRVAEQEMIRHARELGPSIRADGLRKVL
EGVTTIEEVLRVTSDDRRSGQGDRRQGGDRRGTARDRRATDR

Nucleotide


Download         Length: 1569 bp        

>NTDB_id=20436 PSJM300_00765 AFN76237.1 164793..166361(+) (pilB) [Pseudomonas stutzeri DSM 10701]
ATGTCATCCCTCGAAGGTACGTTGACGCCCGCCCGCCTGCCCTTCGCTTTCGCCAGACGCCACGGGGTTTTCCTGCGCGA
TGGCGAAAGCGGCCTGCGGCTGTGCGCCCGTGAAGGCGCGCCGCTGACGGCGCTGCAGGAAGCGCAGCGCGTGGCTGGTG
GCCCCCTGCCTATGCAGTGGCTGACGCCGGAGGCGTTCGACGAGGCGTTGGGCAACGCCTATCAGCACGACTCTTCCGAC
GCCATGCTGATGGTCGAAGGGTTGGGCAAGGACATGGATCTGCACAGCCTGGCCGACCATATCGAGAAGACCGAGGACCT
GCTGGAGCAGGAGGACGACGCGCCGATCATCCGCCTGATCAATGCGCTGCTGGGCGAGGCGATCAGGGAGAACGCGTCCG
ACATCCATGTCGAGACCTTCGAGAAGCGCCTGGTGATCCGCTTCCGGGTCGACGGCATCCTGCGCGAGGTGGTGCAGCCC
AAGCGCGAACTGGCGGCGCTGCTGGTGTCGCGGATCAAGGTCATGGCGCGGCTGGACATTGCCGAGAAGCGCGTGCCTCA
GGATGGGCGGATTTCCCTGCGGGTCGGTGGGCGCGAGGTCGATATCCGCGTGTCGACGCTGCCGTCGGCCAACGGCGAAC
GGGTGGTGCTGCGCCTGCTCGACAAGCAGGCCGGGCGCCTGACCCTGCAGCACCTGGGCATGAGCGAGCGCGACCGCCAG
CGAATGGAGCAGGCGGTAAAGAGGCCCCACGGCATCATCCTGGTCACCGGTCCCACCGGCTCGGGCAAGACCACCACCTT
GTACGCCAGCCTGACCACGCTCAATGACCGCACCCGCAACATCCTCACCGTCGAGGATCCGATCGAATACAACCTCGAAG
GCATTGGCCAGACCCAGGTCAACACCAAGGTCGACATGACCTTCGCCCGCGGACTGCGTGCCATCCTGCGCCAGGACCCG
GATGTGGTGATGGTCGGCGAGATCCGCGACCAGGAGACCGCCGACATGGCGGTGCAGGCCTCGCTCACCGGCCACCTGGT
GCTCTCCACGCTGCACACCAACAGCGCCATCGGTGCGGTGACGCGGCTGGTGGACATGGGCGTCGAGCCCTTCCTGATCT
CCTCGTCGCTGCTCGGTGTGCTGGCCCAGCGCCTCGTGCGGGTGCTGTGTAATGACTGCAAACGCGCCTATGTCGCTGAC
GAAGCCGAGTGTGCGCTGTTCGGCATCGACCCGAGCGAGGCGCCAACGCTCTACCACGCCGAAGGTTGCGATGTGTGCCG
CCAGCTGGGTTATCGCGGGCGGACCGGCATCTACGAGCTGGTGCTGTTCGACGACAGCCTGCGCAGCATGATTCATACCC
GGGTGGCCGAGCAGGAAATGATCCGCCACGCGCGCGAGCTGGGGCCGAGCATCCGTGCCGATGGCCTGCGCAAGGTGCTC
GAAGGCGTGACCACCATCGAAGAGGTGCTGCGTGTGACCAGCGATGACCGACGCTCCGGCCAGGGCGATCGCAGACAGGG
CGGCGATCGCCGTGGCACTGCCCGTGACCGCAGGGCCACCGACCGATGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-388)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

48.418

78.736

0.381
  pilB/pilB1 Synechocystis sp. PCC 6803

46.209

80.843

0.374
  pilB Vibrio parahaemolyticus RIMD 2210633

48.111

76.054

0.366
  pilB Acinetobacter baumannii D1279779

45.763

79.119

0.362
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.586

74.521

0.362
  pilF Neisseria gonorrhoeae MS11

47.716

75.479

0.36
  pilB Vibrio campbellii strain DS40M4

47.475

75.862

0.36
  pilB Legionella pneumophila strain ERS1305867

48.205

74.713

0.36

Multiple sequence alignment