Detailed information    

experimental Experimentally validated

Overview

Name   eppA   Type   Machinery gene
Locus tag   MMJJ_RS04105 Genome accession   NZ_CP026606
Coordinates   761839..762879 (-) Length   346 a.a.
NCBI ID   WP_104838584.1    Uniprot ID   A0A2L1CA81
Organism   Methanococcus maripaludis strain DSM 2067     
Function   DNA uptake   
DNA binding and uptake

Function

The ΔeppA mutant showed a defect in DNA uptake, indicating that prepilin peptidase EppA is essential for this process in M. maripaludis strain JJ.


Genomic Context

Location: 756839..767879
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MMJJ_RS09430 (MMJJ_08220) - 757742..757918 (-) 177 WP_244901564.1 hypothetical protein -
  MMJJ_RS04075 (MMJJ_08230) - 757920..758438 (-) 519 WP_104837794.1 hypothetical protein -
  MMJJ_RS04080 (MMJJ_08240) - 758404..758853 (-) 450 WP_104837795.1 hypothetical protein -
  MMJJ_RS04085 (MMJJ_08250) - 758831..759247 (-) 417 WP_104837796.1 class III signal peptide-containing protein -
  MMJJ_RS04090 (MMJJ_08260) - 759281..760174 (-) 894 WP_104837797.1 hypothetical protein -
  MMJJ_RS04095 (MMJJ_08270) - 760174..761322 (-) 1149 WP_104837798.1 DUF515 domain-containing protein -
  MMJJ_RS04100 (MMJJ_08280) - 761448..761837 (-) 390 WP_104837799.1 class III signal peptide-containing protein -
  MMJJ_RS04105 (MMJJ_08290) eppA 761839..762879 (-) 1041 WP_104838584.1 A24 family peptidase C-terminal domain-containing protein Machinery gene
  MMJJ_RS04110 (MMJJ_08300) - 762893..763261 (-) 369 WP_104837800.1 cysteine-rich small domain-containing protein -
  MMJJ_RS04115 (MMJJ_08310) - 763343..764029 (+) 687 WP_104837801.1 HDIG domain-containing metalloprotein -
  MMJJ_RS04120 (MMJJ_08320) - 764070..764801 (+) 732 WP_104837802.1 amino acid ABC transporter ATP-binding protein -
  MMJJ_RS04125 (MMJJ_08330) - 764830..765951 (-) 1122 WP_104837803.1 tRNA (guanine(10)-N(2))-dimethyltransferase -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38740.28 Da        Isoelectric Point: 6.4832

>NTDB_id=56 MMJJ_RS04105 WP_104838584.1 761839..762879(-) (eppA) [Methanococcus maripaludis strain DSM 2067]
MFGFDNLILGVYLFNFLLILTATYTDIKERIIPHFVIILMLIVNLPLGYYFFGFDAITSFFATLILCLILGVGMGGGDVK
MFTALSPLFAAETIYFVPKNILILIGLSALFAAIFPMTKILKNYWKDIVPSSAYLAMLIGIIVSITEIYSIGNTKTILWI
YIILSIFISRKIPKYRIISNKLGYITPIYLVGFYLLNPAYFVSENVLISFFVYIGQLSLISLVIYALTGAEISSKKQISD
LKEGDIIRDIVTIKENGEVTVENANILKRFKHMIYGEMGKLEGKSLMTDGEGLSDENLELLNKLQNEGKIGGELNVLTTY
PFVPFVLVAYCVITLLNMGFINYLVG

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=56 MMJJ_RS04105 WP_104838584.1 761839..762879(-) (eppA) [Methanococcus maripaludis strain DSM 2067]
ATGTTTGGATTTGATAATTTAATTTTAGGAGTTTATTTATTTAATTTTTTACTGATACTGACTGCAACATATACTGATAT
CAAAGAGCGAATAATTCCCCATTTTGTAATTATATTAATGTTAATTGTTAATTTACCACTCGGGTATTATTTTTTTGGTT
TTGATGCCATAACTTCGTTTTTTGCAACGTTAATTTTATGTTTAATTCTTGGGGTCGGCATGGGTGGTGGAGACGTTAAG
ATGTTTACGGCACTATCTCCTCTTTTTGCAGCAGAAACTATATATTTCGTTCCGAAAAATATTTTAATATTAATCGGTTT
AAGCGCATTGTTTGCGGCAATTTTTCCGATGACAAAAATATTAAAAAATTACTGGAAAGATATTGTTCCATCTTCCGCAT
ATCTTGCAATGCTTATTGGAATAATAGTTTCAATTACAGAAATATATTCTATTGGAAATACCAAGACAATACTATGGATT
TACATAATTTTATCGATATTTATTTCCCGAAAAATTCCAAAATATCGAATTATATCTAATAAACTTGGATATATTACGCC
TATTTACTTGGTTGGATTTTATTTACTCAATCCTGCGTATTTTGTATCCGAAAATGTTCTTATTTCGTTTTTTGTGTATA
TTGGACAGCTTTCCTTAATTTCTCTGGTAATATACGCATTAACAGGTGCTGAAATATCTTCAAAAAAACAAATCAGCGAT
TTAAAAGAGGGAGATATAATTCGGGACATTGTAACAATAAAAGAAAATGGCGAAGTAACTGTTGAAAATGCAAATATTTT
GAAGCGTTTCAAGCACATGATTTATGGAGAAATGGGTAAACTCGAAGGAAAATCGTTAATGACTGATGGAGAAGGTCTTT
CGGATGAAAATCTGGAATTATTAAATAAACTGCAAAATGAAGGGAAAATCGGCGGGGAATTAAATGTTTTAACTACGTAT
CCGTTTGTTCCATTTGTGCTTGTTGCTTACTGTGTAATTACCCTACTAAATATGGGCTTTATAAATTATCTGGTGGGATA
A

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(14-114)

Archaeal Peptidase A24 C-terminal Domain (Arc_PepC)

(210-316)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2L1CA81

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Dallas R Fonseca et al. (2020) Type IV-Like Pili Facilitate Transformation in Naturally Competent Archaea. Journal of Bacteriology 202(21):e00355-20. [PMID: 32817089]