Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   PSJM300_00225 Genome accession   CP003725
Coordinates   49041..50138 (+) Length   365 a.a.
NCBI ID   AFN76129.1    Uniprot ID   -
Organism   Pseudomonas stutzeri DSM 10701     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 44041..55138
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSJM300_00205 - 44955..46301 (-) 1347 AFN76125.1 16S rRNA methyltransferase B -
  PSJM300_00210 fmt 46298..47239 (-) 942 AFN76126.1 methionyl-tRNA formyltransferase -
  PSJM300_00215 def 47284..47790 (-) 507 AFN76127.1 peptide deformylase -
  PSJM300_00220 tsaP 47938..48963 (+) 1026 AFN76128.1 hypothetical protein Machinery gene
  PSJM300_00225 dprA 49041..50138 (+) 1098 AFN76129.1 DNA processing protein DprA Machinery gene
  PSJM300_00230 - 50199..50756 (+) 558 AFN76130.1 hypothetical protein -
  PSJM300_00235 - 50850..51827 (-) 978 AFN76131.1 quinone oxidoreductase -
  PSJM300_00240 - 51975..52889 (+) 915 AFN76132.1 coproporphyrinogen III oxidase -
  PSJM300_00245 aroE 52926..53744 (+) 819 AFN76133.1 shikimate 5-dehydrogenase -
  PSJM300_00250 - 53752..54363 (-) 612 AFN76134.1 putative NADH-flavin reductase -
  PSJM300_00255 - 54379..54690 (-) 312 AFN76135.1 hypothetical protein -

Sequence

Protein


Download         Length: 365 a.a.        Molecular weight: 38408.87 Da        Isoelectric Point: 5.9962

>NTDB_id=20434 PSJM300_00225 AFN76129.1 49041..50138(+) (dprA) [Pseudomonas stutzeri DSM 10701]
MPAISPAELEARLRLHLLPELGPRRLHKLLSAFPDASSALSAPAGAWRSLGLPASCAEPRRSEAIRERAAAALAWLEQPD
HHLLMWDDPGYPALLAELPDAPPLLFVEGEPALLERPQLAMVGSRRASRPGMDNARSFARSLGGAGFVITSGLALGIDGA
AHQGALDAGGQTVAVLGTGLQRLYPARHTGLASSIVEGGGALVSELPLDCPPQAANFPRRNRIISGLSLGVLVVEASPSS
GSLITARLAAEQGREVYAIPGSIHHPGARGCHQLIRQGATLVESVEDILQALRGWQQLAPAPQMEEPERRQGHLLLDQLL
AAPMSSEALAQALGQSLAQVLAELTELEIEGRVACENGQWSARAG

Nucleotide


Download         Length: 1098 bp        

>NTDB_id=20434 PSJM300_00225 AFN76129.1 49041..50138(+) (dprA) [Pseudomonas stutzeri DSM 10701]
ATGCCTGCCATTTCCCCCGCCGAGCTCGAAGCGCGGCTGCGCCTTCATCTGCTGCCAGAGCTCGGACCGCGTCGCCTGCA
CAAGCTGCTCAGTGCCTTCCCCGATGCCTCTTCCGCGCTCAGCGCCCCGGCCGGTGCCTGGCGCTCCCTGGGCCTGCCAG
CCAGTTGCGCCGAACCAAGACGCAGTGAAGCGATCCGCGAACGTGCGGCTGCTGCGTTGGCCTGGCTGGAGCAGCCCGAT
CACCATCTGCTGATGTGGGACGATCCCGGTTACCCCGCGCTGCTGGCCGAGCTGCCGGACGCGCCGCCGCTGTTGTTCGT
CGAGGGCGAGCCGGCACTGCTGGAGCGTCCGCAACTGGCCATGGTGGGCAGCCGTCGCGCCTCGCGCCCGGGTATGGACA
ACGCGCGGAGCTTCGCTCGTAGCCTGGGCGGTGCCGGTTTCGTCATCACCAGCGGCCTGGCGCTGGGCATCGATGGCGCC
GCGCATCAGGGCGCGCTGGATGCTGGTGGCCAAACCGTCGCGGTACTGGGCACTGGGCTGCAGCGGCTCTACCCGGCACG
GCATACCGGCCTGGCCAGCAGCATCGTCGAAGGCGGTGGCGCGCTGGTGTCCGAACTGCCGCTGGATTGCCCGCCGCAGG
CGGCCAACTTTCCTCGTCGCAACAGAATCATCAGTGGGCTGTCGCTGGGTGTGCTGGTGGTCGAGGCGAGTCCTTCCAGC
GGCTCGCTGATCACCGCCCGCCTGGCCGCCGAGCAGGGGCGCGAGGTCTACGCCATTCCGGGCTCGATCCACCATCCGGG
CGCTCGCGGCTGCCACCAATTGATCCGTCAGGGCGCGACGCTGGTCGAGAGCGTCGAGGATATCCTGCAAGCCTTGCGTG
GTTGGCAGCAACTTGCACCGGCCCCACAGATGGAGGAGCCCGAGCGACGGCAAGGTCACCTGCTGCTCGATCAACTGCTG
GCCGCGCCGATGAGCAGCGAAGCCCTGGCCCAGGCGCTTGGCCAGTCATTGGCGCAGGTGCTGGCGGAGCTGACCGAACT
GGAAATCGAGGGGCGGGTGGCCTGTGAGAACGGCCAGTGGAGCGCCCGCGCCGGTTGA

Domains

Predicted by InterProScan.

DNA recombination-mediator protein A (DNA_processg_A)

(76-283)

DprA winged helix domain (WHD_DprA)

(300-358)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Vibrio campbellii strain DS40M4

46.322

100

0.466
  dprA Legionella pneumophila strain ERS1305867

44.413

95.616

0.425
  dprA Vibrio cholerae strain A1552

49.306

78.904

0.389
  dprA Vibrio cholerae O1 biovar El Tor strain E7946

49.306

78.904

0.389
  dprA Glaesserella parasuis strain SC1401

48.276

79.452

0.384
  dprA Neisseria meningitidis strain C311

46.309

81.644

0.378
  dprA Neisseria meningitidis MC58

46.309

81.644

0.378
  dprA Haemophilus influenzae Rd KW20

49.11

76.986

0.378
  dprA Neisseria gonorrhoeae strain FA1090

45.215

83.014

0.375
  dprA Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.027

100

0.375
  dprA Neisseria gonorrhoeae MS11

44.884

83.014

0.373
  dprA Thermus thermophilus HB27

40.867

88.493

0.362

Multiple sequence alignment