Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   TM_RS02935 Genome accession   NC_000853
Coordinates   599293..600177 (-) Length   294 a.a.
NCBI ID   WP_004081293.1    Uniprot ID   A0A0F6AJU4
Organism   Thermotoga maritima MSB8     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 594293..605177
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TM_RS02905 (TM0564) - 595224..595703 (+) 480 WP_010865148.1 flavin reductase family protein -
  TM_RS02910 (TM0565) sfsA 595700..596368 (+) 669 WP_004081303.1 DNA/RNA nuclease SfsA -
  TM_RS02915 (TM0566) - 596372..596566 (+) 195 WP_004081301.1 hypothetical protein -
  TM_RS02920 (TM0567) - 596585..596998 (-) 414 WP_004081299.1 Rrf2 family transcriptional regulator -
  TM_RS02925 (TM0568) - 597000..598664 (-) 1665 WP_004081297.1 peptidoglycan DD-metalloendopeptidase family protein -
  TM_RS02930 (TM0569) - 598640..599296 (-) 657 WP_004081295.1 DUF2225 domain-containing protein -
  TM_RS02935 (TM0570) pilA 599293..600177 (-) 885 WP_004081293.1 signal recognition particle-docking protein FtsY Machinery gene
  TM_RS02940 (TM0571) - 600291..601670 (+) 1380 WP_004081291.1 DegQ family serine endoprotease -
  TM_RS02945 (TM0572) - 601767..602918 (+) 1152 WP_004081290.1 DegT/DnrJ/EryC1/StrS family aminotransferase -
  TM_RS02950 (TM0573) lnt 602911..604374 (+) 1464 WP_004081289.1 apolipoprotein N-acyltransferase -

Sequence

Protein


Download         Length: 294 a.a.        Molecular weight: 32046.26 Da        Isoelectric Point: 5.3215

>NTDB_id=20421 TM_RS02935 WP_004081293.1 599293..600177(-) (pilA) [Thermotoga maritima MSB8]
MGLFDFLKKGLQKTKETFFGRVVKLLKGKKLDDETREELEELLIQADVGVETTEYILERLEEKDGDALESLKEIILEILN
FDTKLNVPPEPPFVIMVVGVNGTGKTTSCGKLAKMFVDEGKSVVLAAADTFRAAAIEQLKIWGERVGATVISHSEGADPA
AVAFDAVAHALARNKDVVIIDTAGRLHTKKNLMEELRKVHRVVKKKIPDAPHETLLVIDATTGQNGLVQAKIFKEAVNVT
GIILTKLDGTAKGGITLAIARELGIPIKFIGVGEKAEDLRPFDPEAFVEVLLSE

Nucleotide


Download         Length: 885 bp        

>NTDB_id=20421 TM_RS02935 WP_004081293.1 599293..600177(-) (pilA) [Thermotoga maritima MSB8]
ATGGGACTCTTCGATTTTTTGAAAAAGGGACTCCAGAAGACGAAAGAAACGTTTTTTGGACGAGTGGTGAAGCTCCTCAA
AGGAAAAAAGCTGGACGATGAAACCCGCGAGGAGCTGGAAGAACTTCTCATTCAAGCGGACGTGGGAGTCGAGACAACGG
AATACATTCTGGAGAGATTGGAAGAGAAGGACGGAGACGCTCTCGAGTCTCTCAAGGAAATCATTCTGGAAATTCTGAAC
TTCGATACGAAGCTGAACGTTCCTCCTGAACCCCCGTTCGTGATCATGGTTGTTGGTGTCAACGGAACGGGAAAGACCAC
CTCCTGTGGAAAGCTCGCAAAGATGTTCGTGGATGAAGGAAAGAGTGTGGTGCTCGCTGCGGCGGATACTTTCAGAGCAG
CCGCCATAGAACAGCTGAAGATATGGGGAGAACGTGTCGGTGCCACGGTGATATCCCACTCGGAAGGAGCAGATCCGGCC
GCCGTTGCCTTTGACGCGGTGGCTCACGCTCTCGCCAGAAACAAAGATGTGGTCATAATAGACACCGCCGGAAGACTTCA
CACGAAGAAGAATCTCATGGAGGAATTGCGGAAGGTTCACAGAGTTGTCAAGAAGAAGATCCCCGACGCTCCGCACGAAA
CGCTCCTCGTGATAGACGCCACCACCGGCCAAAACGGACTTGTTCAGGCAAAAATATTCAAAGAAGCAGTGAATGTCACC
GGTATAATTCTCACAAAGCTCGACGGAACCGCGAAAGGCGGAATAACTCTGGCCATTGCCCGGGAACTGGGAATTCCCAT
AAAGTTCATCGGTGTCGGAGAAAAGGCAGAAGACCTGAGGCCTTTCGATCCTGAGGCCTTTGTGGAGGTATTGCTTTCAG
AATGA

Domains

Predicted by InterproScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(22-65)

SRP54-type protein, GTPase domain (SRP54)

(92-292)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0F6AJU4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

50.331

100

0.517

Multiple sequence alignment