Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   K04M1_RS13540 Genome accession   NZ_CP017891
Coordinates   2853893..2854357 (+) Length   154 a.a.
NCBI ID   WP_033905592.1    Uniprot ID   A0AAW9B0I7
Organism   Vibrio alginolyticus strain K04M1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2848893..2859357
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K04M1_RS13525 (K04M1_26230) pdhR 2850926..2851693 (-) 768 WP_005379989.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  K04M1_RS13530 (K04M1_26240) ampD 2852099..2852650 (-) 552 WP_042521337.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K04M1_RS13535 (K04M1_26250) nadC 2852743..2853630 (+) 888 WP_005379983.1 carboxylating nicotinate-nucleotide diphosphorylase -
  K04M1_RS13540 (K04M1_26260) pilA 2853893..2854357 (+) 465 WP_033905592.1 pilin Machinery gene
  K04M1_RS13545 (K04M1_26270) pilB 2854361..2856046 (+) 1686 WP_054730502.1 type IV-A pilus assembly ATPase PilB Machinery gene
  K04M1_RS13550 (K04M1_26280) pilC 2856070..2857293 (+) 1224 WP_054730505.1 type II secretion system F family protein Machinery gene
  K04M1_RS13555 (K04M1_26290) pilD 2857358..2858227 (+) 870 WP_086047031.1 prepilin peptidase Machinery gene
  K04M1_RS13560 (K04M1_26300) coaE 2858228..2858842 (+) 615 WP_017635920.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 16098.26 Da        Isoelectric Point: 7.6284

>NTDB_id=203709 K04M1_RS13540 WP_033905592.1 2853893..2854357(+) (pilA) [Vibrio alginolyticus strain K04M1]
MKNSKQKKQQGFTLIELMIVVAIIGILAAFAVPAYQNYTKKATLAEFPKAASAMKLAVELCAHENASNAATFKTNCLSGS
NGVPSKINNLNDISIEAVAGSSGVNVIAKAVAVKGPIASGEQYTMASNYTTSGIEWAGSCQDSNGNNQSDYCPE

Nucleotide


Download         Length: 465 bp        

>NTDB_id=203709 K04M1_RS13540 WP_033905592.1 2853893..2854357(+) (pilA) [Vibrio alginolyticus strain K04M1]
ATGAAAAACAGTAAACAGAAAAAACAGCAAGGTTTTACCTTGATTGAATTAATGATTGTCGTCGCGATTATTGGTATTTT
GGCCGCATTTGCTGTGCCTGCTTATCAAAACTACACAAAAAAAGCGACGCTGGCAGAGTTCCCAAAAGCAGCTTCTGCGA
TGAAGTTAGCCGTTGAGTTGTGTGCACATGAAAATGCATCAAACGCAGCTACTTTTAAAACGAACTGCTTATCTGGTAGT
AATGGTGTACCTTCAAAAATTAACAACCTAAATGATATCTCTATTGAAGCAGTTGCTGGATCATCAGGCGTAAATGTCAT
TGCAAAAGCTGTAGCAGTAAAAGGGCCGATTGCTAGTGGAGAACAGTATACTATGGCATCTAACTACACGACTAGTGGTA
TTGAGTGGGCGGGCTCATGTCAAGATTCTAATGGTAATAACCAATCTGATTACTGTCCTGAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio campbellii strain DS40M4

55.484

100

0.558

Multiple sequence alignment