Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   BKP58_RS21255 Genome accession   NZ_CP017763
Coordinates   3979407..3980174 (+) Length   255 a.a.
NCBI ID   WP_014480292.1    Uniprot ID   -
Organism   Bacillus subtilis strain 29R7-12     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 3974407..3985174
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BKP58_RS21225 (BKP58_21180) pgpH 3974703..3976838 (+) 2136 WP_014480296.1 cyclic-di-AMP phosphodiesterase PgpH -
  BKP58_RS21230 (BKP58_21185) ybeY 3976839..3977312 (+) 474 WP_014480295.1 rRNA maturation RNase YbeY -
  BKP58_RS21235 (BKP58_21190) unpK 3977293..3977664 (+) 372 WP_014480294.1 diacylglycerol kinase family protein -
  BKP58_RS21240 (BKP58_21195) - 3977783..3978193 (+) 411 WP_014480293.1 cytidine deaminase -
  BKP58_RS21245 (BKP58_21200) era 3978174..3979079 (+) 906 WP_003230051.1 GTPase Era -
  BKP58_RS21250 (BKP58_21205) yqzL 3979227..3979370 (+) 144 WP_003230053.1 YqzL family protein -
  BKP58_RS21255 (BKP58_21210) recO 3979407..3980174 (+) 768 WP_014480292.1 DNA repair protein RecO Machinery gene
  BKP58_RS21260 (BKP58_21215) glyQ 3980471..3981358 (+) 888 WP_003226213.1 glycine--tRNA ligase subunit alpha -
  BKP58_RS21265 (BKP58_21220) glyS 3981351..3983390 (+) 2040 WP_014480291.1 glycine--tRNA ligase subunit beta -
  BKP58_RS21270 (BKP58_21225) ccpN 3983523..3984161 (+) 639 WP_003237058.1 transcriptional regulator CcpN -
  BKP58_RS21275 (BKP58_21230) ppsR 3984187..3984999 (+) 813 WP_003230064.1 pyruvate, water dikinase regulatory protein -

Sequence

Protein


Download         Length: 255 a.a.        Molecular weight: 29342.05 Da        Isoelectric Point: 8.3080

>NTDB_id=202703 BKP58_RS21255 WP_014480292.1 3979407..3980174(+) (recO) [Bacillus subtilis strain 29R7-12]
MLTKCEGIVLRTNDYGETNKIVTLLTREHGKIGVMARGAKKPNSRLSAVSQPFLYGSFLMQKTSGLGTLQQGEMILSMRG
IREDLFLTAYAAYIAELVDRGTEEKKPNPYLFEFILESLKQLNEGTDPDVITFIVQMKMLGVMGLYPELNHCVHCKSQDG
TFHFSVRDNGFICHRCFEKDPYRIPLKPQTARLLRLFYYFDLSRLGNVSLKEETKAELKQVIDLYYEEYSGLYLKSKRFL
DQMESMKHLMGENKS

Nucleotide


Download         Length: 768 bp        

>NTDB_id=202703 BKP58_RS21255 WP_014480292.1 3979407..3980174(+) (recO) [Bacillus subtilis strain 29R7-12]
ATGCTGACAAAATGTGAAGGAATCGTTCTTCGCACAAATGATTACGGAGAGACGAATAAAATCGTAACTTTACTGACAAG
AGAACACGGAAAAATAGGTGTTATGGCACGAGGCGCCAAAAAACCGAACAGCCGTTTATCAGCAGTCAGCCAGCCCTTTT
TGTATGGTTCTTTCTTAATGCAAAAAACATCGGGCCTCGGAACGCTTCAGCAGGGGGAAATGATCCTCAGCATGAGAGGC
ATCAGGGAAGACTTGTTTTTAACAGCATATGCTGCCTATATTGCTGAGTTAGTCGACAGAGGCACAGAAGAAAAAAAGCC
AAATCCTTATTTATTTGAATTCATATTAGAATCATTAAAACAGCTGAATGAAGGAACAGATCCGGATGTTATCACCTTCA
TTGTTCAAATGAAGATGCTCGGTGTCATGGGCCTGTATCCTGAGCTGAATCATTGTGTACATTGTAAGAGCCAAGACGGA
ACCTTTCATTTCTCTGTTCGTGATAATGGTTTTATATGCCACCGCTGTTTTGAAAAAGATCCTTACAGGATACCGCTCAA
ACCGCAAACGGCAAGGCTGTTAAGACTCTTTTACTATTTTGATCTGTCGAGGCTCGGCAATGTGTCCTTAAAAGAGGAAA
CAAAAGCTGAATTAAAGCAAGTGATTGATTTATATTACGAAGAATATTCGGGATTGTATTTAAAATCAAAGCGCTTTTTA
GATCAAATGGAAAGCATGAAACATCTTATGGGTGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O C terminal (RecO_C)

(82-237)

Recombination protein O N terminal (RecO_N)

(1-75)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

99.216

100

0.992

Multiple sequence alignment