Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   BJN34_RS02410 Genome accession   NZ_CP017757
Coordinates   503776..505257 (-) Length   493 a.a.
NCBI ID   WP_078195190.1    Uniprot ID   A0A1U9UJK9
Organism   Cupriavidus necator strain NH9     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 498776..510257
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BJN34_RS02380 (BJN34_02355) - 499109..499768 (-) 660 WP_078195185.1 LON peptidase substrate-binding domain-containing protein -
  BJN34_RS02385 (BJN34_02360) rapZ 499850..500734 (-) 885 WP_078195186.1 RNase adapter RapZ -
  BJN34_RS02390 (BJN34_02365) - 500843..501229 (-) 387 WP_078195187.1 PsiF family protein -
  BJN34_RS02395 (BJN34_02370) hprK 501415..502386 (-) 972 WP_078195188.1 HPr(Ser) kinase/phosphatase -
  BJN34_RS02400 (BJN34_02375) - 502467..502922 (-) 456 WP_078195189.1 PTS sugar transporter subunit IIA -
  BJN34_RS02405 (BJN34_02380) raiA 503377..503727 (-) 351 WP_010814386.1 ribosome-associated translation inhibitor RaiA -
  BJN34_RS02410 (BJN34_02385) rpoN/rpoN1 503776..505257 (-) 1482 WP_078195190.1 RNA polymerase factor sigma-54 Machinery gene
  BJN34_RS02415 (BJN34_02390) lptB 505393..506193 (-) 801 WP_078195191.1 LPS export ABC transporter ATP-binding protein -
  BJN34_RS02420 (BJN34_02395) lptA 506251..506898 (-) 648 WP_078195192.1 lipopolysaccharide transport periplasmic protein LptA -
  BJN34_RS02425 (BJN34_02400) lptC 507013..507642 (-) 630 WP_078195193.1 LPS export ABC transporter periplasmic protein LptC -
  BJN34_RS02430 (BJN34_02405) - 507683..508666 (-) 984 WP_078198446.1 KpsF/GutQ family sugar-phosphate isomerase -

Sequence

Protein


Download         Length: 493 a.a.        Molecular weight: 54816.09 Da        Isoelectric Point: 4.9117

>NTDB_id=202553 BJN34_RS02410 WP_078195190.1 503776..505257(-) (rpoN/rpoN1) [Cupriavidus necator strain NH9]
MKPSLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVEQALTENPLLERENDWIESPLRVAADGSVNVQSAPAPAPAEPQ
GNGDARADGAGDDDSYGDNSSGDDYGSSDWSLDDFARRPQGDEDEKTPMQLREAEPTLREYLMEQLTPLKISTRDKGLAI
FLIESLDDDGYLSASLEEICTELPEELEFELDEVHAILTLLQSFDPPGVGARNAAECLALQLRRLKHPQRELALNIVTNH
LELLAVRDYTRLKKALQVDEVALKAAHDLIRSLAPYPGHAYSRPEADFVVPDVFVRKSGGGWIAQLNPDVMPRLRINDMY
AQILRGAKGESGTAGLQQKLQEARWLIKNIQQRFDTILRVSQAIVERQKNFFTHGEIAMRPLVLREIADTLGLHESTISR
VTTNKYMATPMGTFELKYFFGSHVSTETGGAASSTAIRALIKQLIGAEDPRNPLSDSRIAELLGEQGFVVARRTVAKYRE
ALKIPAVNLRKSL

Nucleotide


Download         Length: 1482 bp        

>NTDB_id=202553 BJN34_RS02410 WP_078195190.1 503776..505257(-) (rpoN/rpoN1) [Cupriavidus necator strain NH9]
ATGAAACCGTCGCTACAGCTCCGCCTCTCCCAGCATCTGGCACTGACCCCGCAGCTGCAACAGTCGATCCGGCTGCTGCA
GCTGTCCACGCTGGAACTGCAGCAGGAAGTCGAACAGGCCCTGACGGAAAACCCGCTGCTCGAGCGGGAGAACGACTGGA
TCGAAAGCCCGCTGCGCGTGGCCGCCGATGGCTCGGTCAATGTCCAGAGCGCACCGGCGCCCGCCCCCGCCGAACCCCAG
GGCAATGGCGACGCCCGCGCCGACGGCGCGGGTGACGACGACAGCTATGGCGACAACAGCAGCGGCGACGACTACGGCAG
CAGCGACTGGAGCCTGGACGACTTTGCCCGCCGCCCGCAAGGCGACGAGGACGAAAAGACGCCGATGCAGCTGCGCGAAG
CCGAGCCCACGCTGCGCGAATACCTGATGGAGCAGCTGACGCCGCTGAAGATCTCGACACGCGACAAGGGCCTGGCCATC
TTCCTGATCGAATCGCTCGATGACGATGGCTACCTGAGTGCCTCGCTTGAAGAGATCTGCACGGAGTTGCCTGAAGAACT
TGAATTCGAGCTCGACGAAGTCCACGCGATCCTGACGCTCTTGCAGAGCTTCGATCCGCCGGGGGTGGGCGCGCGCAATG
CCGCCGAGTGCCTGGCGCTGCAACTGCGCCGCCTGAAGCACCCGCAGCGCGAGCTGGCGCTGAACATCGTCACCAATCAC
CTGGAGTTGCTGGCCGTACGCGACTACACGCGGCTGAAGAAGGCGCTGCAGGTCGATGAAGTAGCGCTGAAGGCCGCCCA
CGACCTGATCCGTTCGCTCGCGCCCTATCCGGGCCATGCCTACAGCCGCCCGGAAGCCGATTTCGTGGTCCCGGATGTGT
TCGTGCGCAAGAGCGGCGGCGGCTGGATCGCGCAGCTCAACCCGGATGTGATGCCGAGGCTGCGCATCAACGATATGTAT
GCACAAATCCTGCGTGGCGCGAAGGGGGAGTCTGGCACCGCGGGGCTGCAGCAGAAGCTGCAGGAGGCACGCTGGCTGAT
CAAGAATATCCAGCAAAGGTTCGATACGATCCTGCGTGTCTCGCAAGCCATTGTCGAGCGTCAAAAGAACTTTTTCACCC
ACGGTGAAATCGCCATGCGCCCCTTGGTTTTGCGTGAGATTGCCGATACACTGGGTTTACACGAGTCAACCATCTCCCGG
GTGACGACCAATAAATACATGGCAACGCCGATGGGTACTTTCGAACTGAAGTACTTCTTCGGCAGCCACGTGTCCACCGA
AACCGGTGGCGCGGCTTCATCAACGGCCATCCGCGCCTTGATCAAGCAACTGATAGGAGCCGAAGACCCGAGGAATCCCC
TTTCCGACAGTCGCATTGCCGAACTGCTGGGTGAGCAAGGCTTCGTTGTCGCACGACGCACCGTCGCCAAGTATCGCGAG
GCCCTGAAGATCCCCGCAGTCAATCTCCGCAAGTCTTTGTAG

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(336-491)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(130-320)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1U9UJK9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

79.641

100

0.809

Multiple sequence alignment