Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   BKK81_RS09575 Genome accession   NZ_CP017751
Coordinates   2164702..2166063 (+) Length   453 a.a.
NCBI ID   WP_071012400.1    Uniprot ID   A0A1D9GU90
Organism   Cupriavidus sp. USMAHM13 isolate pure     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2159702..2171063
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BKK81_RS09560 (BKK81_09560) - 2160595..2161668 (+) 1074 WP_335582864.1 DUF1853 family protein -
  BKK81_RS09565 (BKK81_09565) lplT 2161767..2163086 (-) 1320 WP_071012396.1 lysophospholipid transporter LplT -
  BKK81_RS09570 (BKK81_09570) alr 2163579..2164700 (+) 1122 WP_071012398.1 alanine racemase -
  BKK81_RS09575 (BKK81_09575) radA 2164702..2166063 (+) 1362 WP_071012400.1 DNA repair protein RadA Machinery gene
  BKK81_RS09580 (BKK81_09580) - 2166060..2166680 (+) 621 WP_083384028.1 GNAT family N-acetyltransferase -
  BKK81_RS09585 (BKK81_09585) - 2166840..2167325 (+) 486 WP_071012402.1 disulfide bond formation protein B -
  BKK81_RS09590 (BKK81_09590) - 2167407..2169350 (-) 1944 WP_071012404.1 ATP-binding cassette domain-containing protein -
  BKK81_RS09595 (BKK81_09595) - 2169548..2170354 (+) 807 WP_071012405.1 hypothetical protein -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 48390.68 Da        Isoelectric Point: 7.2390

>NTDB_id=202517 BKK81_RS09575 WP_071012400.1 2164702..2166063(+) (radA) [Cupriavidus sp. USMAHM13 isolate pure]
MAKVKTVYTCTECGGTVPRWQGQCPHCQQWNTLVESVAESASAKRFQPLAASTRVRKLAEIEAADVPRFSSGIDEFDRVL
GGGLVAGGVVLIGGDPGIGKSTLLLQALANLAAQRPVLYVSGEESGAQIALRAQRLGVESPALGLLAEIQLEKIQATLEA
ERPEVAVIDSIQTLYSEALTSAPGSVAQVRECAAQLTRIAKSSGTTIILVGHVTKEGSLAGPRVLEHIVDTVLYFEGDTH
SSHRLIRAFKNRFGAVNELGVFAMTERGLRGISNPSALFLSQHEQTVPGSCVLVTQEGTRPLLVEIQALVDTAHVPNPRR
LAVGLEQNRLALLLAVLHRHAGVACFDQDVFLNAVGGVKITEPAADLAVLLSIHSSMRNKPLPRGLVVFGEVGLAGEIRP
SPRGQERLKEAAKLGFTQAVIPKANAPRQPIDGLEVIAVERIEQAIDRVRHLE

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=202517 BKK81_RS09575 WP_071012400.1 2164702..2166063(+) (radA) [Cupriavidus sp. USMAHM13 isolate pure]
TTGGCCAAGGTCAAGACGGTCTACACGTGTACCGAATGCGGCGGCACCGTGCCGCGCTGGCAGGGGCAGTGCCCGCACTG
CCAGCAGTGGAACACCCTGGTGGAGAGCGTGGCGGAAAGCGCTTCGGCCAAGCGCTTCCAGCCGCTCGCGGCGTCGACCA
GGGTGCGCAAGCTGGCGGAGATCGAGGCTGCCGACGTGCCGCGTTTCTCCAGCGGCATCGACGAGTTCGACCGCGTGCTG
GGCGGTGGCCTGGTGGCCGGCGGCGTGGTGCTGATCGGCGGCGACCCGGGCATCGGCAAGTCGACGCTGCTGCTGCAGGC
GCTGGCCAATCTCGCGGCCCAGCGCCCGGTGCTCTATGTCAGCGGCGAGGAGTCAGGCGCCCAGATCGCGCTGCGGGCGC
AGCGCCTGGGCGTGGAAAGCCCGGCGCTCGGCCTGCTGGCGGAGATCCAACTGGAGAAGATCCAGGCCACGCTGGAGGCC
GAGCGGCCCGAGGTGGCGGTGATCGATTCGATCCAGACCCTGTACTCCGAGGCGCTGACCTCGGCCCCCGGGTCGGTGGC
GCAGGTGCGCGAGTGCGCGGCGCAGCTCACGCGCATCGCCAAGAGCAGCGGCACCACCATTATCCTGGTGGGCCACGTGA
CCAAGGAGGGCAGCCTGGCCGGTCCGCGCGTGCTCGAGCATATCGTCGATACCGTGCTGTATTTCGAGGGCGACACGCAT
TCGTCGCATCGCCTGATCCGCGCCTTCAAGAACCGCTTCGGCGCGGTCAACGAGCTCGGCGTGTTCGCCATGACCGAGCG
CGGCCTGCGCGGCATCAGCAACCCGTCGGCGCTGTTCCTGTCTCAGCACGAGCAGACCGTGCCCGGTTCCTGCGTGCTGG
TGACGCAGGAGGGCACGCGCCCGCTGCTGGTCGAGATCCAGGCGCTGGTCGATACCGCCCACGTGCCCAATCCGCGCCGC
CTCGCCGTGGGCCTGGAACAGAACCGGCTGGCGCTGCTGCTCGCGGTACTGCACCGTCACGCCGGCGTTGCCTGCTTCGA
CCAGGATGTCTTCCTCAACGCGGTCGGCGGCGTGAAGATCACCGAGCCGGCGGCCGACCTTGCGGTGCTGCTTTCCATTC
ATTCCTCGATGCGCAACAAGCCGCTGCCGCGCGGCCTGGTGGTGTTCGGCGAGGTCGGGCTGGCGGGCGAGATCCGGCCG
AGCCCGCGCGGCCAGGAGCGCCTCAAGGAGGCCGCCAAGCTGGGCTTCACGCAGGCGGTGATTCCCAAGGCCAATGCGCC
GCGCCAGCCGATCGACGGCCTTGAGGTGATCGCCGTGGAGCGCATCGAGCAGGCCATCGACCGCGTGCGCCATCTCGAAT
GA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(61-217)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1D9GU90

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

50.331

100

0.503
  radA Streptococcus mitis SK321

47.007

99.558

0.468
  radA Streptococcus pneumoniae Rx1

46.593

100

0.468
  radA Streptococcus pneumoniae D39

46.593

100

0.468
  radA Streptococcus pneumoniae R6

46.593

100

0.468
  radA Streptococcus pneumoniae TIGR4

46.593

100

0.468
  radA Streptococcus mitis NCTC 12261

46.785

99.558

0.466

Multiple sequence alignment