Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   BKK81_RS04310 Genome accession   NZ_CP017751
Coordinates   949460..950935 (-) Length   491 a.a.
NCBI ID   WP_071010918.1    Uniprot ID   -
Organism   Cupriavidus sp. USMAHM13 isolate pure     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 944460..955935
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BKK81_RS04280 (BKK81_04280) - 944948..945622 (-) 675 WP_083383927.1 LON peptidase substrate-binding domain-containing protein -
  BKK81_RS04285 (BKK81_04285) rapZ 945689..946576 (-) 888 WP_071010914.1 RNase adapter RapZ -
  BKK81_RS04290 (BKK81_04290) - 946687..946992 (-) 306 WP_071016037.1 PsiF family protein -
  BKK81_RS04295 (BKK81_04295) hprK 947169..948140 (-) 972 WP_071010915.1 HPr(Ser) kinase/phosphatase -
  BKK81_RS04300 (BKK81_04300) ptsN 948220..948675 (-) 456 WP_071010916.1 PTS IIA-like nitrogen regulatory protein PtsN -
  BKK81_RS04305 (BKK81_04305) raiA 949060..949410 (-) 351 WP_071010917.1 ribosome-associated translation inhibitor RaiA -
  BKK81_RS04310 (BKK81_04310) rpoN/rpoN1 949460..950935 (-) 1476 WP_071010918.1 RNA polymerase factor sigma-54 Machinery gene
  BKK81_RS04315 (BKK81_04315) lptB 951036..951830 (-) 795 WP_071010919.1 LPS export ABC transporter ATP-binding protein -
  BKK81_RS04320 (BKK81_04320) lptA 951886..952512 (-) 627 WP_071016040.1 lipopolysaccharide transport periplasmic protein LptA -
  BKK81_RS04325 (BKK81_04325) lptC 952570..953199 (-) 630 WP_071010920.1 LPS export ABC transporter periplasmic protein LptC -
  BKK81_RS04330 (BKK81_04330) - 953216..954199 (-) 984 WP_071016041.1 KpsF/GutQ family sugar-phosphate isomerase -

Sequence

Protein


Download         Length: 491 a.a.        Molecular weight: 54490.82 Da        Isoelectric Point: 4.9230

>NTDB_id=202511 BKK81_RS04310 WP_071010918.1 949460..950935(-) (rpoN/rpoN1) [Cupriavidus sp. USMAHM13 isolate pure]
MKPSLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVEQALIENPLLERENDWIESPLRVAADGSINVQSAPAPAPAEAQ
GNGEARSDEGGGDEERFGDGADDYGSDWSLDDFARRPQGDEDEKAPLQLRDAEPTLREHLMEQLAPLKISARDKGLAIFL
IESLDDDGYLSATLEEICEELPEELEFEIEEVQAMLALLQSFDPPGVGARNAAECLALQLRRLSHPQRELALGIVNHHLE
LLAARDYTRLKKALQVDETALKAAHDLIRSLAPYPGHAYNRPQADFVVPDVFVRKSGSGWIAQLNPDVMPRLRINDMYAQ
ILRGAKGESGAAGLQQKLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFSHGEIAMRPLVLREIADTLGLHESTISRVT
TNKYMATPMGTFELKYFFGSHVSTETGGAASSTAIRALIKQLIGAEDPKNPLSDSRIAELLGDQGFVVARRTVAKYREAL
KIPAVNLRKSL

Nucleotide


Download         Length: 1476 bp        

>NTDB_id=202511 BKK81_RS04310 WP_071010918.1 949460..950935(-) (rpoN/rpoN1) [Cupriavidus sp. USMAHM13 isolate pure]
ATGAAACCTTCGCTTCAGCTCCGCCTTTCCCAGCATCTCGCACTGACGCCACAGCTTCAGCAGTCGATCCGGCTGTTGCA
GCTTTCCACGCTCGAACTGCAGCAGGAAGTCGAGCAGGCCCTGATCGAAAACCCTCTGCTCGAGCGCGAAAACGATTGGA
TCGAGAGCCCGCTGCGGGTGGCCGCCGATGGCAGCATCAACGTGCAGAGCGCACCCGCGCCGGCCCCGGCCGAAGCGCAG
GGCAATGGCGAGGCACGCAGCGACGAGGGCGGCGGCGATGAAGAGCGCTTCGGCGACGGCGCCGACGACTACGGCAGCGA
CTGGAGCCTGGACGACTTCGCGCGTCGCCCCCAGGGCGACGAAGACGAGAAGGCGCCGCTGCAACTGCGCGACGCCGAGC
CGACCCTGCGCGAGCACCTGATGGAACAGCTCGCGCCGCTGAAGATCTCGGCGCGCGACAAGGGCCTGGCCATCTTCCTG
ATCGAGTCGCTCGACGACGACGGCTACCTCAGCGCCACGCTCGAGGAGATCTGCGAAGAGCTGCCCGAAGAGCTGGAGTT
CGAGATCGAGGAAGTGCAGGCCATGCTGGCCCTGCTGCAGAGCTTCGATCCACCCGGCGTGGGAGCGCGCAATGCGGCCG
AATGCCTGGCGCTGCAGCTGCGGCGGCTGTCGCATCCGCAGCGCGAGCTGGCGCTGGGCATCGTCAACCACCACCTGGAA
CTGCTGGCCGCGCGCGATTACACGCGCTTGAAGAAGGCGCTGCAGGTGGACGAGACGGCGCTGAAGGCAGCACATGACCT
GATCCGCTCGCTGGCACCGTATCCGGGCCACGCCTACAACCGTCCGCAGGCCGACTTCGTCGTGCCCGACGTGTTCGTGC
GCAAGAGCGGCAGCGGCTGGATCGCCCAACTCAATCCCGACGTGATGCCGCGCCTGCGCATCAACGACATGTACGCGCAG
ATCCTGCGCGGCGCCAAGGGCGAATCCGGTGCCGCCGGCCTGCAGCAGAAACTGCAGGAAGCCCGCTGGCTGATCAAGAA
CATCCAGCAGCGCTTCGACACCATCCTGCGTGTCGCACAGGCCATTGTCGAGCGTCAAAAGAACTTCTTCAGCCACGGTG
AAATTGCGATGCGCCCCTTGGTTTTGCGGGAGATTGCCGATACACTGGGATTACACGAGTCAACCATCTCTCGGGTGACG
ACTAACAAATACATGGCCACCCCCATGGGCACTTTCGAGCTGAAGTACTTCTTCGGCAGCCATGTGTCCACGGAAACGGG
CGGCGCTGCTTCATCAACGGCTATCCGAGCCTTGATCAAGCAACTGATAGGAGCCGAAGACCCGAAGAATCCCCTGTCCG
ACAGCCGAATTGCCGAATTGCTGGGCGATCAGGGCTTCGTCGTGGCGCGCCGGACGGTCGCCAAGTACCGAGAAGCGCTG
AAGATCCCGGCAGTCAATCTCCGCAAGTCTTTGTAG

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(334-489)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(129-318)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

78.156

100

0.794

Multiple sequence alignment