Detailed information NTG0202510

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: BKK81_RS04195
• Genome accession: NZ_CP017751
• Coordinates: 928137..929231 (-)
• Length: 364 a.a.
• NCBI ID: WP_071010901.1
• Uniprot ID: A0A1D9GW63
• Organism: Cupriavidus sp. USMAHM13 isolate pure
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 923137..934231

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BKK81_RS04175 (BKK81_04175)	-	924385..925263 (+)	879	WP_071010899.1	pirin family protein	-
BKK81_RS04180 (BKK81_04180)	ybiB	925316..926326 (-)	1011	WP_071016027.1	DNA-binding protein YbiB	-
BKK81_RS04185 (BKK81_04185)	-	926671..927372 (+)	702	WP_071010900.1	fumarylacetoacetate hydrolase family protein	-
BKK81_RS04190 (BKK81_04190)	maiA	927379..928023 (+)	645	WP_071016029.1	maleylacetoacetate isomerase	-
BKK81_RS04195 (BKK81_04195)	pilA	928137..929231 (-)	1095	WP_071010901.1	signal recognition particle-docking protein FtsY	Machinery gene
BKK81_RS04200 (BKK81_04200)	-	929292..930809 (+)	1518	WP_071010902.1	pitrilysin family protein	-
BKK81_RS04205 (BKK81_04205)	-	930882..932189 (+)	1308	WP_231908023.1	pitrilysin family protein	-
BKK81_RS04210 (BKK81_04210)	rsmD	932239..932898 (+)	660	WP_083383926.1	16S rRNA (guanine(966)-N(2))-methyltransferase RsmD	-
BKK81_RS04215 (BKK81_04215)	coaD	933123..933608 (+)	486	WP_071010904.1	pantetheine-phosphate adenylyltransferase	-
BKK81_RS04220 (BKK81_04220)	-	933670..933930 (+)	261	WP_071010905.1	YfhL family 4Fe-4S dicluster ferredoxin	-

Sequence

Protein

• Length: 364 a.a.
• Molecular weight: 39067.09 Da
• Isoelectric Point: 6.3706

>NTDB_id=202510 BKK81_RS04195 WP_071010901.1 928137..929231(-) (pilA) [Cupriavidus sp. USMAHM13 isolate pure]
MFSFWKKRKAEAAPAPAPAPASAPAPSPATPQETLPAHAVPAPVPEPAATADAYELTLPPVPTQEDRQGWMRRLRAGLSK
TSRNLGTLFLGVKVDEALFEELETALLMADAGVEATEYLLGELRRRVKNERIEDAEGVKAALRTLLTDLLRPLEKTMELG
REQPLVMMIAGVNGAGKTTSIGKLCKHFQHYGQSVLLAAGDTFRAAAREQLTIWGERNNVTVVAQESGDPAAVIFDAVNA
ARARGIDIVMADTAGRLPTQLHLMEELKKVRRVIAKAMPTAPHEVLLVIDANTGQNALAQVRAFDDALGLTGLLVTKLDG
TAKGGILAAIARQRPVPVYFIGVGEKVEDLQPFKAEEFADALLG

Nucleotide

• Length: 1095 bp

>NTDB_id=202510 BKK81_RS04195 WP_071010901.1 928137..929231(-) (pilA) [Cupriavidus sp. USMAHM13 isolate pure]
ATGTTTAGTTTCTGGAAGAAGCGCAAGGCGGAAGCGGCTCCCGCACCCGCCCCGGCACCCGCCAGCGCGCCTGCGCCCTC
CCCCGCCACGCCGCAGGAAACCCTGCCGGCGCACGCCGTGCCCGCGCCGGTTCCCGAGCCGGCGGCCACGGCGGACGCCT
ATGAACTGACCCTGCCGCCGGTGCCCACGCAGGAAGACCGGCAGGGCTGGATGCGCCGGCTGCGCGCCGGCCTGTCCAAG
ACCAGCCGCAATCTCGGCACGCTGTTCCTCGGCGTCAAGGTCGACGAGGCCCTGTTCGAGGAACTCGAAACCGCGCTGCT
GATGGCCGATGCCGGCGTCGAAGCGACCGAGTACCTGCTGGGCGAGCTGCGCCGCCGCGTCAAGAACGAGCGCATCGAGG
ATGCCGAAGGCGTCAAGGCCGCGCTGCGCACGCTGCTGACCGACCTGCTGCGTCCGCTCGAGAAGACCATGGAGCTGGGC
CGCGAACAGCCGCTGGTGATGATGATCGCGGGCGTCAACGGCGCCGGCAAGACCACCAGCATCGGCAAGCTGTGCAAGCA
CTTCCAGCACTACGGGCAGTCGGTGCTGCTGGCCGCCGGCGACACCTTCCGCGCCGCCGCGCGGGAGCAGTTGACGATCT
GGGGCGAGCGCAACAATGTCACGGTGGTGGCGCAGGAAAGCGGCGACCCCGCGGCCGTGATCTTCGACGCCGTCAACGCG
GCGCGCGCGCGCGGCATCGACATCGTGATGGCCGATACCGCGGGGCGCCTGCCCACGCAGCTGCACCTGATGGAAGAGCT
GAAGAAGGTCCGGCGCGTCATCGCCAAGGCCATGCCGACGGCGCCGCACGAGGTGCTGCTGGTGATCGACGCCAACACAG
GCCAGAACGCGCTGGCCCAGGTGCGCGCCTTCGACGATGCGCTGGGCCTGACCGGCCTGCTGGTGACCAAGCTGGACGGC
ACCGCCAAGGGCGGCATCCTCGCCGCCATCGCGCGACAGCGCCCGGTGCCGGTCTATTTCATCGGCGTGGGCGAGAAGGT
CGAGGATCTGCAGCCGTTCAAGGCCGAAGAGTTCGCCGACGCGCTGCTGGGCTGA

Domains

Predicted by InterproScan.

• SRP54-type protein, GTPase domain (SRP54) (165-364)

• SRP54-type protein, helical bundle domain (SRP54_N) (88-143)

3D structure

• AlphaFold DB: A0A1D9GW63

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Neisseria gonorrhoeae MS11	63.072	84.066	0.53