Detailed information NTG0202497

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: BKK79_RS08725
• Genome accession: NZ_CP017748
• Coordinates: 1993072..1994166 (+)
• Length: 364 a.a.
• NCBI ID: WP_071010901.1
• Uniprot ID: A0A1D9GW63
• Organism: Cupriavidus sp. USMAA2-4 isolate pure
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1988072..1999166

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BKK79_RS08700 (BKK79_08700)	-	1988373..1988633 (-)	261	WP_071010905.1	YfhL family 4Fe-4S dicluster ferredoxin	-
BKK79_RS08705 (BKK79_08705)	coaD	1988695..1989180 (-)	486	WP_071010904.1	pantetheine-phosphate adenylyltransferase	-
BKK79_RS08710 (BKK79_08710)	rsmD	1989405..1990064 (-)	660	WP_083383926.1	16S rRNA (guanine(966)-N(2))-methyltransferase RsmD	-
BKK79_RS08715 (BKK79_08715)	-	1990114..1991421 (-)	1308	WP_231908023.1	pitrilysin family protein	-
BKK79_RS08720 (BKK79_08720)	-	1991494..1993011 (-)	1518	WP_071037544.1	pitrilysin family protein	-
BKK79_RS08725 (BKK79_08725)	pilA	1993072..1994166 (+)	1095	WP_071010901.1	signal recognition particle-docking protein FtsY	Machinery gene
BKK79_RS08730 (BKK79_08730)	maiA	1994280..1994924 (-)	645	WP_071016029.1	maleylacetoacetate isomerase	-
BKK79_RS08735 (BKK79_08735)	-	1994931..1995632 (-)	702	WP_071010900.1	fumarylacetoacetate hydrolase family protein	-
BKK79_RS08740 (BKK79_08740)	ybiB	1995976..1996986 (+)	1011	WP_071038475.1	DNA-binding protein YbiB	-
BKK79_RS08745 (BKK79_08745)	-	1997039..1997917 (-)	879	WP_071010899.1	pirin family protein	-

Sequence

Protein

• Length: 364 a.a.
• Molecular weight: 39067.09 Da
• Isoelectric Point: 6.3706

>NTDB_id=202497 BKK79_RS08725 WP_071010901.1 1993072..1994166(+) (pilA) [Cupriavidus sp. USMAA2-4 isolate pure]
MFSFWKKRKAEAAPAPAPAPASAPAPSPATPQETLPAHAVPAPVPEPAATADAYELTLPPVPTQEDRQGWMRRLRAGLSK
TSRNLGTLFLGVKVDEALFEELETALLMADAGVEATEYLLGELRRRVKNERIEDAEGVKAALRTLLTDLLRPLEKTMELG
REQPLVMMIAGVNGAGKTTSIGKLCKHFQHYGQSVLLAAGDTFRAAAREQLTIWGERNNVTVVAQESGDPAAVIFDAVNA
ARARGIDIVMADTAGRLPTQLHLMEELKKVRRVIAKAMPTAPHEVLLVIDANTGQNALAQVRAFDDALGLTGLLVTKLDG
TAKGGILAAIARQRPVPVYFIGVGEKVEDLQPFKAEEFADALLG

Nucleotide

• Length: 1095 bp

>NTDB_id=202497 BKK79_RS08725 WP_071010901.1 1993072..1994166(+) (pilA) [Cupriavidus sp. USMAA2-4 isolate pure]
ATGTTTAGTTTCTGGAAGAAGCGCAAGGCGGAAGCGGCTCCCGCACCCGCCCCGGCACCCGCCAGCGCGCCTGCGCCCTC
CCCCGCCACGCCGCAGGAAACCCTGCCGGCGCACGCCGTGCCCGCGCCGGTTCCCGAGCCGGCGGCCACGGCGGACGCCT
ATGAACTGACCCTGCCGCCGGTGCCCACGCAGGAAGACCGACAGGGCTGGATGCGCCGGCTGCGCGCCGGCCTGTCCAAG
ACCAGCCGCAATCTCGGCACGCTGTTCCTCGGCGTCAAGGTCGACGAGGCCCTGTTCGAGGAACTCGAAACCGCGCTGCT
GATGGCCGATGCCGGCGTCGAAGCGACCGAGTACCTGCTGGGCGAGCTGCGCCGCCGCGTCAAGAACGAGCGCATCGAGG
ATGCCGAAGGCGTCAAGGCCGCGCTGCGCACGCTGCTGACCGACCTGCTGCGTCCGCTCGAGAAGACCATGGAGCTGGGC
CGCGAACAGCCGCTGGTGATGATGATCGCGGGCGTCAACGGCGCCGGCAAGACCACCAGCATCGGCAAGCTGTGCAAGCA
CTTCCAGCACTACGGGCAGTCGGTGCTGCTGGCCGCCGGCGACACCTTCCGCGCCGCCGCGCGGGAGCAGCTGACGATCT
GGGGCGAGCGCAACAATGTCACGGTGGTGGCGCAGGAAAGCGGCGACCCCGCGGCCGTGATCTTCGACGCCGTCAACGCG
GCGCGCGCGCGCGGCATCGACATCGTGATGGCCGATACCGCGGGGCGCCTGCCCACGCAGCTGCACCTGATGGAAGAGCT
GAAGAAGGTCCGGCGCGTCATCGCCAAGGCCATGCCGACGGCGCCGCACGAGGTGCTGCTGGTGATCGACGCCAACACAG
GCCAGAACGCGCTGGCCCAGGTGCGCGCCTTCGACGATGCGCTGGGCCTGACCGGCCTGCTGGTGACCAAGCTGGACGGC
ACCGCCAAGGGCGGCATCCTCGCCGCCATCGCGCGACAGCGCCCGGTGCCGGTCTATTTCATCGGCGTGGGCGAGAAGGT
CGAGGATCTGCAGCCGTTCAAGGCCGAAGAGTTCGCCGACGCGCTGCTGGGCTGA

Domains

Predicted by InterproScan.

• SRP54-type protein, GTPase domain (SRP54) (165-364)

• SRP54-type protein, helical bundle domain (SRP54_N) (88-143)

3D structure

• AlphaFold DB: A0A1D9GW63

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Neisseria gonorrhoeae MS11	63.072	84.066	0.53