Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   CIW80_RS12785 Genome accession   NZ_CP022686
Coordinates   2551496..2552164 (-) Length   222 a.a.
NCBI ID   WP_001297375.1    Uniprot ID   P65957
Organism   Escherichia coli Nissle 1917     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2546496..2557164
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CIW80_RS12755 (CIW80_12860) rfaQ 2546674..2547732 (-) 1059 WP_000360415.1 lipopolysaccharide core heptosyltransferase RfaQ -
  CIW80_RS12760 (CIW80_12865) waaA 2548145..2549422 (+) 1278 WP_000891564.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  CIW80_RS12765 (CIW80_12870) coaD 2549430..2549909 (+) 480 WP_001171873.1 pantetheine-phosphate adenylyltransferase -
  CIW80_RS12770 (CIW80_12875) mutM 2549948..2550757 (-) 810 WP_001114542.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  CIW80_RS12775 (CIW80_12880) rpmG 2550855..2551022 (-) 168 WP_001051798.1 50S ribosomal protein L33 -
  CIW80_RS12780 (CIW80_12885) rpmB 2551043..2551279 (-) 237 WP_000091955.1 50S ribosomal protein L28 -
  CIW80_RS12785 (CIW80_12890) radC 2551496..2552164 (-) 669 WP_001297375.1 DNA repair protein RadC Machinery gene
  CIW80_RS12790 (CIW80_12895) coaBC 2552336..2553556 (+) 1221 WP_000050139.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  CIW80_RS12795 (CIW80_12900) dut 2553534..2553992 (+) 459 WP_000976070.1 dUTP diphosphatase -
  CIW80_RS12800 (CIW80_12905) slmA 2554099..2554695 (+) 597 WP_000818603.1 nucleoid occlusion factor SlmA -
  CIW80_RS12805 (CIW80_12910) pyrE 2554732..2555373 (-) 642 WP_000806177.1 orotate phosphoribosyltransferase -
  CIW80_RS12810 (CIW80_12915) rph 2555439..2556155 (-) 717 WP_001247093.1 ribonuclease PH -
  CIW80_RS12820 (CIW80_12925) yicC 2556282..2557145 (+) 864 WP_000621330.1 YicC/YloC family endoribonuclease -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25258.35 Da        Isoelectric Point: 7.9748

>NTDB_id=202157 CIW80_RS12785 WP_001297375.1 2551496..2552164(-) (radC) [Escherichia coli Nissle 1917]
MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=202157 CIW80_RS12785 WP_001297375.1 2551496..2552164(-) (radC) [Escherichia coli Nissle 1917]
GTGAAAAACAATGCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACGGATGTCGAGCT
GCTGGCGCTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAGCAATTTAGTGGCGTTCATGGAATTGGCGTGGCGAAATTCGCCCAGTTA
AAGGGGATTGCCGAACTGGCGCGGCGTTACTACAACGTACGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GAGGGAATTTTTACAAAGCCAGCTCACGGGCGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCCCAACACAGGG
TTATAACGCATAGCCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCGCATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

RadC-like JAB domain (RadC)

(103-220)

UPF0758 N-terminal (UPF0758_N)

(9-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P65957

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496