Detailed information NTG0202136

Bioinformatically predicted

Overview

• Name: rpoS
• Type: Regulator
• Locus tag: CIW80_RS07355
• Genome accession: NZ_CP022686
• Coordinates: 1473330..1474322 (-)
• Length: 330 a.a.
• NCBI ID: WP_000081550.1
• Uniprot ID: A0A370V765
• Organism: Escherichia coli Nissle 1917
• Function: regulation of chitinases (predicted from homology) Competence regulation

Genomic Context

Location: 1468330..1479322

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CIW80_RS07335 (CIW80_07385)	ygbM	1468961..1469737 (+)	777	WP_001136915.1	HPr family phosphocarrier protein	-
CIW80_RS07340 (CIW80_07390)	ygbN	1469826..1471190 (+)	1365	WP_000104433.1	GntP family transporter	-
CIW80_RS07345 (CIW80_07395)	-	1471333..1472376 (-)	1044	WP_001161573.1	MBL fold metallo-hydrolase	-
CIW80_RS07350 (CIW80_07400)	-	1472601..1473152 (+)	552	WP_001179443.1	flavodoxin family protein	-
CIW80_RS07355 (CIW80_07405)	rpoS	1473330..1474322 (-)	993	WP_000081550.1	RNA polymerase sigma factor RpoS	Regulator
CIW80_RS07360 (CIW80_07410)	nlpD	1474385..1475479 (-)	1095	WP_306163061.1	murein hydrolase activator NlpD	-
CIW80_RS07365 (CIW80_07415)	pcm	1475664..1476290 (-)	627	WP_000254701.1	protein-L-isoaspartate O-methyltransferase	-
CIW80_RS07370 (CIW80_07420)	surE	1476284..1477045 (-)	762	WP_001295182.1	5'/3'-nucleotidase SurE	-
CIW80_RS07375 (CIW80_07425)	truD	1477026..1478075 (-)	1050	WP_000568929.1	tRNA pseudouridine(13) synthase TruD	-
CIW80_RS07380 (CIW80_07430)	ispF	1478072..1478551 (-)	480	WP_001219237.1	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase	-
CIW80_RS07385 (CIW80_07435)	ispD	1478551..1479261 (-)	711	WP_000246149.1	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase	-

Sequence

Protein

• Length: 330 a.a.
• Molecular weight: 37972.84 Da
• Isoelectric Point: 4.5597

>NTDB_id=202136 CIW80_RS07355 WP_000081550.1 1473330..1474322(-) (rpoS) [Escherichia coli Nissle 1917]
MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFA
RRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILAD
EKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQ
GLNIEALFRE

Nucleotide

• Length: 993 bp

>NTDB_id=202136 CIW80_RS07355 WP_000081550.1 1473330..1474322(-) (rpoS) [Escherichia coli Nissle 1917]
ATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGA
AAAGGCCTTAGTAGAAGAGGAACCCAGTGATAACGATTTGGCCGAAGAGGAACTGTTATCGCAGGGAGCCACACAGCGTG
TGCTGGACGCGACTCAGCTTTACCTTGGTGAGATTGGTTATTCACCACTGTTAACGGCCGAAGAAGAAGTTTATTTTGCG
CGTCGCGCACTGCGTGGAGATGTTGCCTCTCGCCGCCGGATGATCGAGAGTAACTTGCGTCTGGTGGTAAAAATTGCCCG
CCGTTATGGCAATCGTGGTCTGGCGTTGCTGGACCTGATCGAAGAGGGCAACCTGGGGCTGATCCGCGCGGTAGAGAAGT
TTGACCCGGAACGTGGTTTCCGCTTCTCAACATACGCAACCTGGTGGATTCGCCAGACGATCGAACGGGCGATTATGAAC
CAAACCCGTACTATTCGTTTGCCGATTCACATCGTAAAGGAGCTGAACGTTTACCTGCGAACCGCACGTGAGTTGTCCCA
TAAGCTGGACCACGAACCAAGTGCGGAAGAGATCGCAGAGCAACTGGATAAGCCAGTTGATGACGTCAGCCGTATGCTTC
GTCTTAACGAGCGCATTACCTCGGTAGACACCCCGCTGGGTGGTGATTCCGAAAAAGCGTTGCTGGACATCCTGGCCGAT
GAAAAAGAGAATGGTCCGGAAGATACCACGCAAGATGACGATATGAAGCAGAGCATCGTCAAATGGCTGTTCGAGCTGAA
CGCCAAACAGCGTGAAGTACTGGCACGTCGATTCGGTTTGCTGGGGTACGAAGCGGCAACACTGGAAGATGTAGGTCGTG
AAATTGGCCTCACCCGTGAACGTGTTCGCCAGATTCAGGTTGAAGGCCTGCGCCGTTTGCGCGAAATCCTGCAAACGCAG
GGGCTGAATATCGAAGCGCTGTTCCGTGAGTAA

Domains

Predicted by InterProScan.

• Sigma-70 region 3 (Sigma70_r3) (174-248)

• Sigma-70 region 2 (Sigma70_r2) (94-163)

• Sigma-70 factor, region 1.2 (Sigma70_r1_2) (56-88)

• Sigma-70, region 4 (Sigma70_r4) (262-315)

3D structure

• AlphaFold DB: A0A370V765

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rpoS	Vibrio cholerae O1 biovar El Tor strain E7946	73.065	97.879	0.715