Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   BKX93_RS05565 Genome accession   NZ_CP017707
Coordinates   1249942..1251303 (-) Length   453 a.a.
NCBI ID   WP_021477629.1    Uniprot ID   A0A1D9LE15
Organism   Chromobacterium vaccinii strain 21-1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1244942..1256303
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BKX93_RS05535 (BKX93_05535) - 1245764..1246726 (+) 963 WP_046167277.1 hypothetical protein -
  BKX93_RS05540 (BKX93_05540) - 1246762..1247553 (-) 792 WP_070979086.1 transporter substrate-binding domain-containing protein -
  BKX93_RS05545 (BKX93_05545) - 1247814..1248167 (+) 354 WP_070979088.1 DUF2325 domain-containing protein -
  BKX93_RS05550 (BKX93_05550) - 1248216..1249091 (-) 876 WP_070979089.1 hypothetical protein -
  BKX93_RS05555 (BKX93_05555) - 1249186..1249380 (-) 195 WP_021477627.1 zf-HC2 domain-containing protein -
  BKX93_RS05560 (BKX93_05560) - 1249377..1249937 (-) 561 WP_046156552.1 sigma-70 family RNA polymerase sigma factor -
  BKX93_RS05565 (BKX93_05565) radA 1249942..1251303 (-) 1362 WP_021477629.1 DNA repair protein RadA Machinery gene
  BKX93_RS05570 (BKX93_05570) - 1251376..1251909 (-) 534 WP_046156551.1 hypothetical protein -
  BKX93_RS05575 (BKX93_05575) - 1251924..1252685 (-) 762 WP_082113677.1 SDR family oxidoreductase -
  BKX93_RS05580 (BKX93_05580) hpnE 1252682..1253953 (-) 1272 WP_070979091.1 hydroxysqualene dehydroxylase HpnE -
  BKX93_RS05585 (BKX93_05585) hpnD 1253950..1254795 (-) 846 WP_046156549.1 presqualene diphosphate synthase HpnD -
  BKX93_RS05590 (BKX93_05590) hpnC 1254865..1255677 (-) 813 WP_070979093.1 squalene synthase HpnC -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 48712.97 Da        Isoelectric Point: 6.8124

>NTDB_id=202131 BKX93_RS05565 WP_021477629.1 1249942..1251303(-) (radA) [Chromobacterium vaccinii strain 21-1]
MAKNKTVFSCTECGGQSPKWQGQCPHCNAWNTLTEAVAAPAAAGPRFQSWAANVTKVQKLSEVQTEETPRDPSGIDELDR
VLGGGIVRGAVVLIGGDPGIGKSTLLLQALSQIGQNRKVLYVSGEESAQQIALRASRLALDTSSVDLLAEICIENILATL
KREQPEVVVIDSIQTLYTEQVTSAPGSVSQVRECAAQLTRMAKQSGITVLLVGHVTKEGSLAGPRVLEHMVDTVLYFEGD
SHSSYRMIRAIKNRFGAVNELGVFAMTDRGLKGVSNPSAIFLSSYRDDVAGSCVLVTQEGTRPLLVEIQALVDDCHGFQP
KRLTVGLEQNRLAMLLAVLHRHGGVACFDQDVFLNAVGGVKINEPAADLAIILAMVSSLRNKALPEKLVVFGEVGLAGEV
RPVTRGQERLKEAAKLGFTRAIVPSANKPRQEIEGLKVLAVDRLDQAVEYCRE

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=202131 BKX93_RS05565 WP_021477629.1 1249942..1251303(-) (radA) [Chromobacterium vaccinii strain 21-1]
ATGGCCAAGAACAAAACCGTATTCAGCTGCACCGAGTGCGGCGGACAATCGCCCAAGTGGCAGGGCCAGTGCCCGCATTG
CAATGCCTGGAACACGCTGACAGAGGCGGTGGCCGCGCCGGCCGCGGCCGGTCCGCGCTTCCAGTCGTGGGCGGCCAACG
TCACCAAGGTGCAGAAGCTGTCCGAGGTGCAAACCGAGGAAACCCCGCGCGATCCGTCCGGCATCGACGAACTGGACCGG
GTGCTGGGCGGCGGCATCGTCCGCGGCGCGGTGGTGCTGATCGGCGGCGACCCCGGCATCGGCAAGTCCACGCTGCTGTT
GCAGGCGCTGTCGCAGATCGGCCAGAACCGCAAGGTGCTGTACGTGTCCGGCGAGGAATCGGCGCAGCAGATCGCGCTGC
GCGCCTCAAGACTGGCGCTGGATACCAGCAGCGTCGACCTGCTGGCCGAGATCTGCATCGAAAACATCCTGGCCACGCTG
AAGCGCGAACAGCCCGAGGTGGTGGTGATCGACTCGATCCAGACGCTGTACACCGAGCAGGTGACTTCGGCGCCGGGCTC
GGTGTCGCAGGTGCGCGAATGCGCGGCGCAGCTGACGCGGATGGCCAAGCAGAGCGGCATCACCGTGCTATTGGTCGGCC
ACGTCACCAAGGAAGGCTCGCTGGCCGGTCCGCGCGTGCTGGAGCACATGGTGGATACCGTGCTGTATTTCGAGGGCGAT
TCCCATTCCAGCTACCGGATGATACGCGCGATCAAGAACCGCTTCGGCGCGGTCAATGAGCTGGGCGTGTTCGCGATGAC
CGATCGCGGCCTGAAGGGCGTGTCCAACCCGTCGGCCATCTTCCTCTCATCGTACCGGGATGACGTCGCCGGATCCTGCG
TGCTGGTGACGCAGGAAGGCACGCGGCCGCTGCTGGTGGAGATCCAGGCGCTGGTGGACGATTGCCACGGCTTCCAGCCC
AAGCGCCTCACCGTCGGCCTGGAGCAGAACCGGCTGGCGATGCTGCTGGCGGTGCTGCACCGCCACGGCGGCGTGGCCTG
CTTCGATCAGGACGTGTTCCTCAACGCGGTGGGCGGCGTCAAGATCAACGAACCGGCGGCCGACCTGGCCATCATCCTGG
CCATGGTTTCGTCCTTGCGCAACAAGGCGCTGCCGGAGAAGCTGGTGGTGTTCGGCGAGGTGGGCCTGGCCGGCGAGGTG
CGGCCGGTGACGCGCGGGCAGGAACGGCTGAAGGAAGCCGCCAAGCTGGGCTTCACCCGCGCCATCGTGCCCAGCGCCAA
CAAGCCGCGCCAGGAAATCGAGGGCCTGAAGGTATTGGCCGTGGACCGCCTGGACCAGGCGGTGGAATACTGCAGGGAGT
AG

Domains

Predicted by InterproScan.

Lon protease (S16) C-terminal proteolytic domain (Lon_C)

(358-446)

AAA domain (AAA_25)

(67-219)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1D9LE15

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

50

100

0.503
  radA Streptococcus mitis SK321

48.009

99.779

0.479
  radA Streptococcus mitis NCTC 12261

47.788

99.779

0.477
  radA Streptococcus pneumoniae TIGR4

50

93.598

0.468
  radA Streptococcus pneumoniae D39

50

93.598

0.468
  radA Streptococcus pneumoniae Rx1

50

93.598

0.468
  radA Streptococcus pneumoniae R6

50

93.598

0.468

Multiple sequence alignment