Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   LA20531_RS04090 Genome accession   NZ_CP017706
Coordinates   779284..780660 (-) Length   458 a.a.
NCBI ID   WP_056939974.1    Uniprot ID   -
Organism   Lactobacillus amylovorus DSM 20531     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 774284..785660
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LA20531_RS04065 (LA20531_03960) - 774319..774864 (-) 546 WP_056939969.1 sigma-70 family RNA polymerase sigma factor -
  LA20531_RS04070 (LA20531_03965) rlmB 775003..775755 (-) 753 WP_056939970.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  LA20531_RS04075 (LA20531_03970) - 775742..776185 (-) 444 WP_056939971.1 Mini-ribonuclease 3 -
  LA20531_RS04080 (LA20531_03975) cysS 776178..777611 (-) 1434 WP_056939972.1 cysteine--tRNA ligase -
  LA20531_RS04085 (LA20531_03980) gltX 777708..779207 (-) 1500 WP_056939973.1 glutamate--tRNA ligase -
  LA20531_RS04090 (LA20531_03985) radA 779284..780660 (-) 1377 WP_056939974.1 DNA repair protein RadA Machinery gene
  LA20531_RS04095 (LA20531_03990) - 780660..781211 (-) 552 WP_056939975.1 dUTP diphosphatase -
  LA20531_RS04100 (LA20531_03995) - 781344..781640 (+) 297 WP_056939976.1 GNAT family N-acetyltransferase -
  LA20531_RS04105 (LA20531_04000) pepC 781703..783052 (+) 1350 WP_056939977.1 aminopeptidase C -
  LA20531_RS04110 (LA20531_04005) - 783116..784051 (-) 936 WP_013437163.1 AAA family ATPase -
  LA20531_RS04115 (LA20531_04010) - 784156..784833 (-) 678 WP_022087068.1 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase -
  LA20531_RS04120 (LA20531_04015) - 784838..785026 (-) 189 WP_013437161.1 hypothetical protein -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 50418.01 Da        Isoelectric Point: 6.1964

>NTDB_id=202115 LA20531_RS04090 WP_056939974.1 779284..780660(-) (radA) [Lactobacillus amylovorus DSM 20531]
MARVKTQYKCRSCGYISASYLGRCPNCGAWNQFEKETEEVQKRSTKATASRLIQKTGVNEPVKLDEIKAEKEERIPTKSE
ELNRVLGGGIVPGSLVLIGGDPGIGKSTLMLQIMSDLSEKYKVLYVSGEESANQIKLRADRLCVGQSNMLLYPETDMHDI
REQINDVKPDFVVIDSIQTMNEPSLDSMTGSASQVREVTSELMKIAKMDAITIFVIGHVTKEGTIAGPKIMEHMVDTVLY
FEGDEHHSYRILHSVKNRFGAANEIGMFEMVNEGLKEVTNPSSIFLDQRLPNSTGSAVVVSLEGTRPLLAEIQALVTPTA
FGYAKRTTSGLDYNRAALLLAVLEKRGNLMLQNQDVYLTATGGIKLNEPAIDLAIVMAVASSYTDKEISPTDCFVGEVGL
TGEVRRVDKIDARVKEAAKVGFKRIFVPRHNMYAGLKDYGIEVIPVSSIPQALKLVFD

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=202115 LA20531_RS04090 WP_056939974.1 779284..780660(-) (radA) [Lactobacillus amylovorus DSM 20531]
ATGGCAAGAGTAAAAACACAATATAAATGTCGCTCCTGTGGCTATATTTCTGCGAGTTACTTGGGTAGATGTCCTAACTG
TGGTGCTTGGAACCAATTTGAAAAAGAGACTGAAGAAGTACAAAAGCGTTCTACTAAGGCTACAGCTAGTCGTTTAATTC
AAAAGACCGGCGTCAATGAACCGGTTAAGTTAGACGAAATTAAGGCAGAAAAAGAAGAAAGAATTCCTACCAAATCTGAA
GAATTAAATCGTGTTTTAGGTGGCGGTATTGTGCCAGGATCGCTTGTCTTAATTGGCGGGGATCCCGGAATTGGTAAATC
TACATTGATGCTGCAGATCATGAGCGATTTATCTGAAAAATATAAAGTACTATATGTTTCAGGGGAAGAATCAGCTAATC
AGATCAAGTTAAGAGCCGATCGACTTTGCGTAGGGCAAAGCAACATGCTGTTATATCCCGAAACTGACATGCATGATATT
CGTGAACAGATTAATGATGTTAAACCTGATTTTGTGGTGATCGATTCCATTCAAACGATGAATGAACCTAGTTTGGACTC
GATGACCGGTTCAGCTTCACAGGTTAGGGAAGTAACCAGTGAGCTCATGAAGATCGCTAAAATGGATGCGATCACGATTT
TTGTTATCGGTCACGTGACTAAAGAAGGGACCATTGCTGGTCCTAAGATTATGGAACACATGGTGGATACAGTACTTTAT
TTTGAGGGGGATGAACACCACTCTTACCGTATCCTGCACTCTGTTAAAAACCGTTTTGGTGCTGCTAACGAAATTGGCAT
GTTTGAGATGGTCAATGAAGGGCTAAAAGAAGTTACTAATCCATCATCTATTTTTCTTGATCAAAGATTGCCTAACTCGA
CTGGTTCAGCTGTCGTTGTTTCGCTTGAAGGAACCCGTCCACTTTTAGCTGAAATCCAAGCTTTGGTAACCCCAACGGCA
TTTGGCTATGCGAAGCGAACAACTTCAGGCCTTGACTACAATCGAGCAGCTCTTTTACTTGCCGTTCTTGAAAAGCGTGG
CAATTTAATGCTGCAAAATCAAGATGTTTATTTAACTGCAACTGGCGGGATTAAACTGAATGAACCGGCAATTGATTTAG
CTATTGTTATGGCTGTGGCATCTAGTTACACAGATAAAGAAATTTCACCAACGGACTGCTTTGTAGGTGAAGTTGGTTTA
ACCGGTGAAGTTCGCCGCGTAGACAAGATCGATGCTAGGGTAAAAGAAGCCGCTAAAGTTGGCTTTAAACGAATCTTTGT
ACCGCGGCACAACATGTATGCTGGTCTTAAAGATTATGGAATCGAAGTAATTCCAGTTTCCAGTATTCCACAAGCATTAA
AATTAGTTTTTGATTAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(66-223)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-34)

Subunit ChlI of Mg-chelatase (ChlI)

(363-431)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis SK321

57.987

99.782

0.579
  radA Streptococcus mitis NCTC 12261

57.987

99.782

0.579
  radA Streptococcus pneumoniae Rx1

57.987

99.782

0.579
  radA Streptococcus pneumoniae D39

57.987

99.782

0.579
  radA Streptococcus pneumoniae R6

57.987

99.782

0.579
  radA Streptococcus pneumoniae TIGR4

57.987

99.782

0.579
  radA Bacillus subtilis subsp. subtilis str. 168

56.044

99.345

0.557

Multiple sequence alignment