Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   BKN48_RS12025 Genome accession   NZ_CP017676
Coordinates   2276763..2277212 (-) Length   149 a.a.
NCBI ID   WP_070548532.1    Uniprot ID   -
Organism   Bacillus subtilis strain VV2     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2271763..2282212
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BKN48_RS11990 (BKN48_11990) yckA 2271915..2272595 (-) 681 WP_003234624.1 amino acid ABC transporter permease -
  BKN48_RS11995 (BKN48_11995) yckB 2272605..2273468 (-) 864 WP_080481234.1 transporter substrate-binding domain-containing protein -
  BKN48_RS21215 - 2273630..2273827 (+) 198 WP_145924794.1 hypothetical protein -
  BKN48_RS12005 (BKN48_12005) yckC 2273846..2274301 (+) 456 WP_064672016.1 RDD family protein -
  BKN48_RS12010 (BKN48_12010) yckD 2274382..2274714 (+) 333 WP_015252849.1 YckD family protein -
  BKN48_RS12015 (BKN48_12015) bglC 2274868..2276301 (+) 1434 WP_070548528.1 6-phospho-beta-glucosidase -
  BKN48_RS12020 (BKN48_12020) nin/comJ 2276338..2276736 (-) 399 WP_070548530.1 DNA-entry nuclease inhibitor Regulator
  BKN48_RS12025 (BKN48_12025) nucA/comI 2276763..2277212 (-) 450 WP_070548532.1 DNA-entry nuclease Machinery gene
  BKN48_RS12030 (BKN48_12030) tlpC 2277380..2279101 (-) 1722 WP_070548534.1 methyl-accepting chemotaxis protein TlpC -
  BKN48_RS12035 (BKN48_12035) hxlB 2279211..2279768 (-) 558 WP_041850679.1 6-phospho-3-hexuloisomerase -
  BKN48_RS12040 (BKN48_12040) hxlA 2279774..2280406 (-) 633 WP_021481819.1 3-hexulose-6-phosphate synthase -
  BKN48_RS12045 (BKN48_12045) hxlR 2280638..2281000 (+) 363 WP_003246265.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16440.35 Da        Isoelectric Point: 4.4397

>NTDB_id=201505 BKN48_RS12025 WP_070548532.1 2276763..2277212(-) (nucA/comI) [Bacillus subtilis strain VV2]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSADQKTSQTKEYDETIAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLQDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=201505 BKN48_RS12025 WP_070548532.1 2276763..2277212(-) (nucA/comI) [Bacillus subtilis strain VV2]
GTGAACATCACGACGGACATCATAAAAACAATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGCCTGATAAA
AGGAGACTTTTTCTCAGCTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATCGCCTTCCCATCTGACCGGT
ATCCCGAAACGGCCAAGCATATTAAGGATGCGATAAATGAGGGGCACTCAGAGGTGTGCACTATCGACAGAGACGGAGCT
GAGGAACGCCGCGAGCAATCATTACAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(53-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

97.987

100

0.98

Multiple sequence alignment