Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   BKN48_RS10635 Genome accession   NZ_CP017676
Coordinates   2014070..2015446 (+) Length   458 a.a.
NCBI ID   WP_070548367.1    Uniprot ID   -
Organism   Bacillus subtilis strain VV2     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2009070..2020446
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BKN48_RS10615 (BKN48_10615) ctsR 2009423..2009887 (+) 465 WP_003225724.1 transcriptional regulator CtsR -
  BKN48_RS10620 (BKN48_10620) mcsA 2009901..2010458 (+) 558 WP_015252993.1 protein-arginine kinase activator protein McsA -
  BKN48_RS10625 (BKN48_10625) mcsB 2010458..2011549 (+) 1092 WP_021482008.1 protein arginine kinase -
  BKN48_RS10630 (BKN48_10630) clpC 2011546..2013978 (+) 2433 WP_014475592.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  BKN48_RS10635 (BKN48_10635) radA 2014070..2015446 (+) 1377 WP_070548367.1 DNA repair protein RadA Machinery gene
  BKN48_RS10640 (BKN48_10640) disA 2015450..2016532 (+) 1083 WP_003225736.1 DNA integrity scanning diadenylate cyclase DisA -
  BKN48_RS10645 (BKN48_10645) yacL 2016648..2017748 (+) 1101 WP_003235014.1 PIN/TRAM domain-containing protein -
  BKN48_RS10650 (BKN48_10650) ispD 2017763..2018461 (+) 699 WP_032726598.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  BKN48_RS10655 (BKN48_10655) ispF 2018454..2018930 (+) 477 WP_070548369.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49439.97 Da        Isoelectric Point: 8.2399

>NTDB_id=201502 BKN48_RS10635 WP_070548367.1 2014070..2015446(+) (radA) [Bacillus subtilis strain VV2]
MAKTKSKFICQSCGYESPKWMGKCPGCGAWNTMVEEMIKKAPANRRAAFSHSVQTVQKPSPITSIETSEEPRVKTQLGEF
NRVLGGGVVKGSLVLIGGDPGIGKSTLLLQVSAQLSGSSNSVLYISGEESVKQTKLRADRLGINNPSLHVLSETDMEYIS
SAIQEMNPAFVVVDSIQTVYQSDITSAPGSVSQVRECTAELMKIAKTKGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNEMGIFEMREEGLTEVLNPSEIFLEERSAGSAGSSITASMEGTRPILVEIQALISPTSF
GNPRRMATGIDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAIVISIASSFRDTPPSPADCFIGEVGLT
GEVRRVSRIEQRVKEAAKLGFKRMIIPAANLDGWTKPKGIEVIGVANVAEALRTSLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=201502 BKN48_RS10635 WP_070548367.1 2014070..2015446(+) (radA) [Bacillus subtilis strain VV2]
ATGGCTAAAACAAAATCGAAATTCATCTGCCAATCCTGCGGCTACGAGTCTCCAAAATGGATGGGGAAATGTCCGGGCTG
CGGTGCTTGGAATACAATGGTGGAAGAAATGATTAAAAAAGCACCGGCCAATCGGAGAGCGGCTTTTTCTCATTCTGTTC
AAACTGTACAGAAACCTTCACCTATTACATCAATCGAAACATCAGAAGAACCCCGCGTCAAAACCCAGCTTGGCGAGTTT
AACAGAGTACTCGGCGGAGGTGTCGTTAAAGGCTCCCTCGTTTTAATTGGCGGTGATCCTGGTATCGGAAAGTCAACGCT
ATTACTGCAGGTTTCCGCTCAATTATCAGGCTCATCAAACAGTGTCCTGTATATTTCGGGAGAAGAATCTGTAAAGCAAA
CGAAGCTGCGAGCAGACCGTCTTGGCATTAATAATCCGTCACTACATGTTTTATCTGAAACCGATATGGAGTATATTTCG
TCTGCTATACAAGAGATGAATCCAGCGTTTGTCGTTGTTGACTCTATTCAAACGGTTTACCAAAGCGATATTACATCGGC
TCCAGGCAGTGTGTCACAGGTCCGAGAATGTACTGCTGAGCTGATGAAAATCGCAAAAACAAAAGGTATTCCGATTTTTA
TCGTAGGGCACGTGACGAAAGAAGGGTCTATTGCAGGTCCGAGACTGTTGGAGCATATGGTTGACACTGTTTTATATTTT
GAGGGAGAACGCCACCATACTTTCCGTATTTTGCGGGCTGTAAAAAACCGTTTCGGATCTACAAACGAAATGGGCATTTT
CGAAATGCGGGAAGAGGGGCTCACTGAGGTTTTGAATCCTTCGGAAATTTTCTTAGAAGAACGCTCTGCTGGTTCCGCAG
GCTCGAGTATCACTGCCTCTATGGAGGGCACTAGACCGATTCTCGTTGAAATTCAGGCGCTCATCTCGCCAACAAGCTTT
GGCAACCCAAGGCGTATGGCAACGGGAATAGACCATAACAGGGTTTCTCTGTTAATGGCTGTGTTAGAAAAAAGAGTTGG
ACTGCTGCTGCAAAATCAGGATGCTTATTTGAAAGTGGCTGGCGGCGTTAAGCTTGATGAACCGGCAATTGATCTTGCGA
TTGTGATTAGTATCGCATCAAGCTTTAGAGACACACCTCCAAGTCCTGCAGATTGTTTTATTGGAGAAGTGGGATTAACC
GGAGAAGTCCGGCGGGTTTCAAGAATTGAACAGCGTGTGAAAGAAGCGGCAAAGCTTGGCTTTAAACGCATGATCATACC
CGCGGCAAATCTGGACGGATGGACAAAACCAAAAGGGATTGAGGTTATCGGAGTAGCAAATGTTGCAGAAGCGCTTCGTA
CTTCATTAGGAGGATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(365-430)

AAA domain (AAA_25)

(72-222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

99.563

100

0.996
  radA Streptococcus pneumoniae Rx1

62.914

98.908

0.622
  radA Streptococcus pneumoniae D39

62.914

98.908

0.622
  radA Streptococcus pneumoniae R6

62.914

98.908

0.622
  radA Streptococcus pneumoniae TIGR4

62.914

98.908

0.622
  radA Streptococcus mitis NCTC 12261

62.914

98.908

0.622
  radA Streptococcus mitis SK321

62.693

98.908

0.62

Multiple sequence alignment