Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   BKN48_RS02335 Genome accession   NZ_CP017676
Coordinates   394347..394757 (-) Length   136 a.a.
NCBI ID   WP_070547559.1    Uniprot ID   -
Organism   Bacillus subtilis strain VV2     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 389347..399757
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BKN48_RS02300 (BKN48_02300) - 389433..389573 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  BKN48_RS02310 (BKN48_02310) yqeF 389879..390610 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  BKN48_RS02315 (BKN48_02315) cwlH 390862..391614 (-) 753 WP_070547555.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  BKN48_RS02320 (BKN48_02320) yqeD 391801..392427 (+) 627 WP_070547556.1 TVP38/TMEM64 family protein -
  BKN48_RS02325 (BKN48_02325) gnd 392447..393340 (-) 894 WP_070547557.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  BKN48_RS02330 (BKN48_02330) yqeB 393592..394314 (+) 723 WP_070547558.1 hypothetical protein -
  BKN48_RS02335 (BKN48_02335) nucA/comI 394347..394757 (-) 411 WP_070547559.1 sporulation-specific Dnase NucB Machinery gene
  BKN48_RS02340 (BKN48_02340) sigK 394953..395681 (+) 729 WP_013308023.1 RNA polymerase sporulation sigma factor SigK -
  BKN48_RS21020 (BKN48_02345) - 395681..395797 (+) 117 WP_070547560.1 hypothetical protein -
  BKN48_RS20570 - 395763..395959 (-) 197 Protein_465 recombinase family protein -
  BKN48_RS21425 (BKN48_02350) - 395917..396095 (-) 179 Protein_466 hypothetical protein -
  BKN48_RS02355 (BKN48_02355) psiE 396635..397051 (-) 417 WP_003246154.1 phosphate-starvation-inducible protein PsiE -
  BKN48_RS02360 (BKN48_02360) yrkQ 397097..398395 (-) 1299 WP_041850088.1 two-component system sensor histidine kinase YrkQ -
  BKN48_RS02365 (BKN48_02365) yrkP 398382..399077 (-) 696 WP_041850089.1 two-component response regulator YrkP -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=201472 BKN48_RS02335 WP_070547559.1 394347..394757(-) (nucA/comI) [Bacillus subtilis strain VV2]
MKKWMAGLFLAAAVLLCLMVPQQLQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=201472 BKN48_RS02335 WP_070547559.1 394347..394757(-) (nucA/comI) [Bacillus subtilis strain VV2]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGCTCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGAGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGTACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529

Multiple sequence alignment