Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   BKM66_RS09530 Genome accession   NZ_CP017666
Coordinates   1979433..1980800 (-) Length   455 a.a.
NCBI ID   WP_014638739.1    Uniprot ID   G7SEN8
Organism   Streptococcus suis strain 0061     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1954082..1981789 1979433..1980800 within 0


Gene organization within MGE regions


Location: 1954082..1981789
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BKM66_RS09415 (BKM66_09175) - 1954082..1954600 (-) 519 WP_112435142.1 DUF3916 domain-containing protein -
  BKM66_RS09420 (BKM66_09180) rnmV 1954597..1955199 (-) 603 WP_162636976.1 ribonuclease M5 -
  BKM66_RS09425 (BKM66_09185) - 1955162..1955944 (-) 783 WP_105184278.1 TatD family hydrolase -
  BKM66_RS09430 (BKM66_09190) rpmH 1956050..1956187 (-) 138 WP_002939016.1 50S ribosomal protein L34 -
  BKM66_RS09435 (BKM66_09195) jag 1956355..1957350 (-) 996 WP_013730624.1 RNA-binding cell elongation regulator Jag/EloR -
  BKM66_RS09440 (BKM66_09200) - 1957370..1958182 (-) 813 WP_024401396.1 YidC/Oxa1 family membrane protein insertase -
  BKM66_RS09445 (BKM66_09205) rnpA 1958166..1958525 (-) 360 WP_004194560.1 ribonuclease P protein component -
  BKM66_RS09450 (BKM66_09210) argH 1958596..1959990 (-) 1395 WP_105184277.1 argininosuccinate lyase -
  BKM66_RS09455 (BKM66_09215) - 1960004..1961197 (-) 1194 WP_105184276.1 argininosuccinate synthase -
  BKM66_RS09460 (BKM66_09220) - 1961390..1961938 (-) 549 WP_012775368.1 hypothetical protein -
  BKM66_RS09465 (BKM66_09225) - 1962008..1962535 (-) 528 WP_015647475.1 transcription repressor NadR -
  BKM66_RS09470 (BKM66_09230) - 1962641..1963516 (-) 876 WP_105184275.1 VOC family protein -
  BKM66_RS09475 (BKM66_09235) - 1963636..1964136 (+) 501 WP_105184274.1 NUDIX hydrolase -
  BKM66_RS09480 (BKM66_09240) mmuM 1964186..1965133 (-) 948 WP_105184273.1 homocysteine S-methyltransferase -
  BKM66_RS09485 (BKM66_09245) - 1965146..1966522 (-) 1377 WP_105184272.1 amino acid permease -
  BKM66_RS09490 (BKM66_09250) gltX 1966661..1968115 (-) 1455 WP_105184271.1 glutamate--tRNA ligase -
  BKM66_RS09495 (BKM66_09255) - 1968293..1969573 (-) 1281 WP_105184270.1 MFS transporter -
  BKM66_RS09500 (BKM66_09260) - 1969665..1970330 (-) 666 WP_105184269.1 hypothetical protein -
  BKM66_RS09505 (BKM66_09265) - 1970335..1970781 (-) 447 WP_024388519.1 LytTR family DNA-binding domain-containing protein -
  BKM66_RS10465 (BKM66_09270) - 1970921..1971112 (-) 192 WP_024411142.1 LPXTG cell wall anchor domain-containing protein -
  BKM66_RS10535 - 1972946..1977571 (-) 4626 Protein_1790 KxYKxGKxW signal peptide domain-containing protein -
  BKM66_RS09520 (BKM66_09280) - 1977834..1978592 (-) 759 WP_002939965.1 TIGR00266 family protein -
  BKM66_RS09525 (BKM66_09285) - 1978816..1979310 (-) 495 WP_013730632.1 carbonic anhydrase -
  BKM66_RS09530 (BKM66_09290) radA 1979433..1980800 (-) 1368 WP_014638739.1 DNA repair protein RadA Machinery gene
  BKM66_RS09535 (BKM66_09295) - 1980807..1981340 (-) 534 WP_044693323.1 histidine phosphatase family protein -
  BKM66_RS09540 (BKM66_09300) - 1981346..1981789 (-) 444 WP_024401380.1 dUTP diphosphatase -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 49873.40 Da        Isoelectric Point: 6.5158

>NTDB_id=201325 BKM66_RS09530 WP_014638739.1 1979433..1980800(-) (radA) [Streptococcus suis strain 0061]
MTIAKKKTTFVCQSCEYHSPKYLGRCPNCGSWSSFVEEVEVAEVKNERVSLTGEKTRPMKLNEVSSIQVARTKTNMEEFN
RVLGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLSTIGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSIRTE
IEKIKPDFLIIDSIQTIMSPDISSVQGSVSQVREVTNELMQIAKTNNIATFIVGHMTKEGTLAGPRTLEHMVDTVLYFEG
ERQHTFRILRAVKNRFGSTNEIGIFEMQSQGLVEVLNPSEVFLEERLDGATGSAIVVTMEGTRPILAEVQALVTPTMFGN
AKRTTTGLDFNRASLIMAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVALASSYKDKPTNPQECFIGEIGLTGE
IRRVNRIEQRINEAAKLGFTKVYAPKNSLTGIKVPKEITVIGVTTIGEVLQKVFK

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=201325 BKM66_RS09530 WP_014638739.1 1979433..1980800(-) (radA) [Streptococcus suis strain 0061]
ATGACCATCGCTAAGAAAAAAACAACCTTTGTCTGTCAATCCTGCGAGTACCACTCGCCCAAGTATCTGGGCCGTTGTCC
CAACTGTGGCTCCTGGTCTAGCTTTGTCGAGGAGGTGGAAGTCGCTGAAGTCAAGAACGAGCGGGTTAGCCTGACAGGTG
AGAAGACCCGACCTATGAAGCTCAATGAAGTGTCCTCCATTCAAGTGGCTCGCACCAAGACCAACATGGAGGAGTTCAAC
CGCGTCCTCGGTGGTGGAGTGGTGCCGGGCAGTCTAGTTCTCATTGGTGGCGATCCAGGGATTGGCAAGTCCACCTTGCT
CCTGCAAGTATCCACTCAGCTGTCTACAATTGGCACGGTCCTCTATGTGTCGGGGGAAGAGTCTGCCCAGCAGATTAAGT
TGCGAGCGGAGCGTTTGGGCGACATTGATAGCGAGTTCTATCTCTATGCGGAGACCAATATGCAGAGCATTCGGACCGAG
ATTGAGAAGATAAAACCAGATTTCCTGATTATCGACTCTATCCAGACCATTATGAGCCCTGACATTTCTAGCGTGCAAGG
CTCTGTCAGTCAAGTCCGTGAAGTGACCAATGAGCTTATGCAGATTGCCAAGACCAACAATATCGCAACCTTTATCGTCG
GCCACATGACCAAGGAAGGTACTCTGGCTGGACCTCGGACCTTGGAGCATATGGTAGACACCGTTCTTTATTTTGAGGGC
GAGCGGCAGCACACCTTCCGTATCTTGCGGGCGGTCAAAAATCGCTTTGGCTCCACCAACGAAATCGGTATTTTTGAAAT
GCAGTCGCAGGGCTTGGTCGAAGTCCTCAATCCTAGTGAGGTCTTTCTGGAAGAGCGTTTGGACGGGGCGACAGGCTCTG
CTATTGTCGTGACCATGGAGGGCACCCGCCCCATCCTTGCGGAAGTGCAGGCTCTGGTGACGCCGACCATGTTTGGCAAT
GCCAAGCGGACCACGACAGGCTTGGATTTCAATCGTGCCAGCTTGATTATGGCAGTTTTGGAAAAACGGGCAGGCCTGCT
CCTCCAAAACCAAGATGCCTACCTTAAGTCAGCAGGCGGTGTCAAACTGGATGAGCCAGCTATTGACCTGGCGGTCGCAG
TTGCCCTCGCTTCCAGTTACAAGGATAAGCCAACTAATCCACAAGAGTGCTTTATAGGCGAAATCGGTCTGACAGGGGAA
ATCCGCCGCGTCAATCGGATTGAACAACGGATTAACGAAGCCGCCAAATTGGGCTTTACCAAGGTCTATGCCCCCAAAAA
TTCCCTGACAGGTATCAAGGTGCCCAAGGAAATCACGGTTATCGGCGTGACCACCATTGGCGAAGTCTTGCAGAAAGTGT
TTAAGTAG

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(10-37)

AAA domain (AAA_25)

(64-220)

Subunit ChlI of Mg-chelatase (ChlI)

(363-427)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G7SEN8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

91.372

99.341

0.908
  radA Streptococcus pneumoniae Rx1

91.15

99.341

0.905
  radA Streptococcus pneumoniae D39

91.15

99.341

0.905
  radA Streptococcus pneumoniae R6

91.15

99.341

0.905
  radA Streptococcus pneumoniae TIGR4

91.15

99.341

0.905
  radA Streptococcus mitis SK321

90.929

99.341

0.903
  radA Bacillus subtilis subsp. subtilis str. 168

62.693

99.56

0.624

Multiple sequence alignment