Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   CGU45_RS11155 Genome accession   NZ_CP022526
Coordinates   2328089..2329582 (+) Length   497 a.a.
NCBI ID   WP_003094357.1    Uniprot ID   A0A0H2ZGR9
Organism   Pseudomonas aeruginosa strain Ocean-1155     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2323089..2334582
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CGU45_RS11125 (CGU45_11125) - 2323301..2324110 (-) 810 WP_003094343.1 ABC transporter ATP-binding protein -
  CGU45_RS11130 (CGU45_11130) kdsD 2324524..2325504 (+) 981 WP_003094347.1 arabinose-5-phosphate isomerase KdsD -
  CGU45_RS11135 (CGU45_11135) - 2325504..2326043 (+) 540 WP_003094350.1 KdsC family phosphatase -
  CGU45_RS11140 (CGU45_11140) lptC 2326052..2326624 (+) 573 WP_003094353.1 LPS export ABC transporter periplasmic protein LptC -
  CGU45_RS11145 (CGU45_11145) lptA 2326611..2327138 (+) 528 WP_003120916.1 lipopolysaccharide transport periplasmic protein LptA -
  CGU45_RS11150 (CGU45_11150) lptB 2327138..2327863 (+) 726 WP_003094355.1 LPS export ABC transporter ATP-binding protein -
  CGU45_RS11155 (CGU45_11155) rpoN/rpoN1 2328089..2329582 (+) 1494 WP_003094357.1 RNA polymerase factor sigma-54 Machinery gene
  CGU45_RS11160 (CGU45_11160) hpf 2329660..2329968 (+) 309 WP_003094359.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  CGU45_RS11165 (CGU45_11165) ptsN 2329982..2330446 (+) 465 WP_094069637.1 PTS IIA-like nitrogen regulatory protein PtsN -
  CGU45_RS11170 (CGU45_11170) rapZ 2330448..2331308 (+) 861 WP_003094361.1 RNase adapter RapZ -
  CGU45_RS11175 (CGU45_11175) - 2331338..2331610 (+) 273 WP_003094362.1 HPr family phosphocarrier protein -
  CGU45_RS11180 (CGU45_11180) - 2331707..2332638 (-) 932 Protein_2173 ZIP family metal transporter -
  CGU45_RS11185 (CGU45_11185) - 2332657..2333268 (-) 612 WP_003098862.1 superoxide dismutase -
  CGU45_RS11190 (CGU45_11190) - 2333281..2333730 (-) 450 WP_003094369.1 hypothetical protein -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 56054.22 Da        Isoelectric Point: 4.4327

>NTDB_id=201300 CGU45_RS11155 WP_003094357.1 2328089..2329582(+) (rpoN/rpoN1) [Pseudomonas aeruginosa strain Ocean-1155]
MKPSLVLKMGQQLTMTPQLQQAIRLLQLSTLDLQQEIQEALESNPMLERQEDGDDFDNSDPLADGAEQAASAPQESPLQE
SATPSVESLDDDQWSERIPSELPVDTAWEDIYQTSASSLPSNDDDEWDFTARTSSGESLHSHLLWQVNLAPMSDTDRMIA
VTIIDSINNDGYLEESLEEILAAIDPELDVELDEVEVVLRRIQQLEPAGIGARNLRECLLLQLRQLPSTTPWLNEALRLV
SDYLDLLGGRDYSQLMRRMKLKEDELRQVIELIQCLHPRPGSQIESSEAEYIVPDVIVRKDNERWLVELNQEAMPRLRVN
ATYAGMVRRADSSADNTFMRNQLQEARWFIKSLQSRNETLMKVATQIVEHQRGFLDYGEEAMKPLVLHDIAEAVGMHEST
ISRVTTQKYMHTPRGIFELKYFFSSHVSTAEGGECSSTAIRAIIKKLVAAENAKKPLSDSKIAGLLEAQGIQVARRTVAK
YRESLGIAPSSERKRLV

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=201300 CGU45_RS11155 WP_003094357.1 2328089..2329582(+) (rpoN/rpoN1) [Pseudomonas aeruginosa strain Ocean-1155]
ATGAAACCATCGCTAGTCCTCAAGATGGGCCAGCAGCTGACGATGACGCCGCAGCTGCAACAGGCCATACGCCTCCTCCA
GCTATCCACCCTGGACCTCCAGCAGGAAATCCAGGAAGCGCTGGAATCCAATCCCATGCTCGAACGCCAGGAAGACGGCG
ACGATTTCGACAACAGCGACCCTCTCGCCGATGGCGCCGAACAGGCGGCCAGCGCGCCCCAGGAGAGCCCTCTCCAGGAA
AGCGCCACGCCCAGCGTCGAAAGCCTCGACGACGACCAGTGGAGCGAACGCATACCCAGCGAGTTGCCGGTGGATACCGC
CTGGGAAGACATCTACCAGACCAGCGCCAGCAGCCTGCCCAGCAACGACGATGACGAATGGGACTTCACCGCGCGCACCA
GCAGCGGCGAGAGCCTGCACAGCCACCTGCTCTGGCAGGTCAACCTGGCACCGATGTCGGACACCGATCGGATGATCGCG
GTGACCATCATCGATAGCATCAACAACGATGGCTATCTCGAAGAGTCCCTCGAAGAAATCCTCGCCGCCATCGATCCGGA
ACTGGACGTCGAACTCGACGAAGTGGAAGTCGTGCTGCGCCGCATCCAGCAACTGGAACCTGCTGGCATCGGCGCCCGCA
ACCTGCGCGAGTGCCTGCTGCTGCAACTGCGCCAGTTGCCGTCCACCACGCCCTGGCTGAACGAAGCGCTGCGCCTGGTA
TCCGACTACCTCGACCTGCTCGGCGGCCGCGACTACAGCCAATTGATGCGGCGCATGAAGCTCAAGGAAGACGAACTGCG
CCAGGTCATCGAGCTGATCCAGTGCCTGCATCCCCGGCCCGGTTCGCAGATCGAATCGAGCGAAGCCGAATACATCGTCC
CCGACGTCATCGTGCGCAAGGACAACGAGCGCTGGCTGGTCGAGCTGAACCAGGAGGCGATGCCGCGCCTGCGCGTCAAC
GCCACCTACGCCGGCATGGTCCGCCGCGCCGACAGCAGCGCCGACAATACCTTCATGCGCAACCAGTTGCAGGAAGCCCG
CTGGTTCATCAAGAGCCTGCAGAGCCGCAACGAAACGCTGATGAAGGTCGCCACCCAGATCGTCGAGCACCAGCGCGGTT
TCCTCGACTACGGCGAAGAAGCCATGAAGCCGCTGGTCCTGCACGACATCGCCGAAGCGGTGGGCATGCACGAATCGACC
ATTTCGCGGGTCACCACGCAGAAATACATGCATACGCCGCGCGGCATTTTCGAGCTGAAATATTTCTTTTCAAGTCACGT
CAGTACCGCTGAAGGCGGGGAATGCTCGTCCACCGCGATCCGCGCGATCATCAAGAAACTGGTGGCCGCGGAAAATGCGA
AAAAGCCATTGAGCGACAGCAAGATCGCTGGTTTACTGGAGGCACAGGGCATTCAAGTGGCTCGCCGCACCGTCGCCAAA
TACCGCGAATCCCTGGGCATAGCCCCTTCGAGCGAGCGCAAGCGACTGGTGTGA

Domains

Predicted by InterProScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(337-495)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(129-323)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2ZGR9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

46.654

100

0.477

Multiple sequence alignment