Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   CEK59_RS19715 Genome accession   NZ_CP022478
Coordinates   4154217..4155710 (+) Length   497 a.a.
NCBI ID   WP_023130255.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain LW     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4149217..4160710
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CEK59_RS19685 (CEK59_19595) - 4149429..4150238 (-) 810 WP_003094343.1 ABC transporter ATP-binding protein -
  CEK59_RS19690 (CEK59_19600) kdsD 4150652..4151632 (+) 981 WP_003094347.1 arabinose-5-phosphate isomerase KdsD -
  CEK59_RS19695 (CEK59_19605) - 4151632..4152171 (+) 540 WP_003094350.1 HAD family hydrolase -
  CEK59_RS19700 (CEK59_19610) lptC 4152180..4152752 (+) 573 WP_003094353.1 LPS export ABC transporter periplasmic protein LptC -
  CEK59_RS19705 (CEK59_19615) lptA 4152739..4153266 (+) 528 WP_003120916.1 lipopolysaccharide transport periplasmic protein LptA -
  CEK59_RS19710 (CEK59_19620) lptB 4153266..4153991 (+) 726 WP_003094355.1 LPS export ABC transporter ATP-binding protein -
  CEK59_RS19715 (CEK59_19625) rpoN/rpoN1 4154217..4155710 (+) 1494 WP_023130255.1 RNA polymerase factor sigma-54 Machinery gene
  CEK59_RS19720 (CEK59_19630) raiA 4155788..4156096 (+) 309 WP_003094359.1 ribosome-associated translation inhibitor RaiA -
  CEK59_RS19725 (CEK59_19635) ptsN 4156110..4156574 (+) 465 WP_003098853.1 PTS IIA-like nitrogen regulatory protein PtsN -
  CEK59_RS19730 (CEK59_19640) rapZ 4156576..4157436 (+) 861 WP_003094361.1 RNase adapter RapZ -
  CEK59_RS19735 (CEK59_19645) - 4157466..4157738 (+) 273 WP_003094362.1 HPr family phosphocarrier protein -
  CEK59_RS19740 (CEK59_19650) - 4157835..4158767 (-) 933 WP_003120918.1 ZIP family metal transporter -
  CEK59_RS19745 (CEK59_19655) - 4158786..4159397 (-) 612 WP_003098862.1 superoxide dismutase -
  CEK59_RS19750 (CEK59_19660) - 4159410..4159859 (-) 450 WP_034026605.1 tetratricopeptide repeat-containing protein -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 56084.24 Da        Isoelectric Point: 4.4327

>NTDB_id=201114 CEK59_RS19715 WP_023130255.1 4154217..4155710(+) (rpoN/rpoN1) [Pseudomonas aeruginosa strain LW]
MKPSLVLKMGQQLTMTPQLQQAIRLLQLSTLDLQQEIQEALESNPMLERQEDGDDFDNSDPLADGAEQAASAPQESPLQE
STTPSVESLDDDQWSERIPSELPVDTAWEDIYQTSASSLPSNDDDEWDFTARTSSGESLHSHLLWQVNLAPMSDTDRMIA
VTIIDSINNDGYLEESLEEILAAIDPELDVELDEVEVVLRRIQQLEPAGIGARNLRECLLLQLRQLPSTTPWLNEALRLV
SDYLDLLGGRDYSQLMRRMKLKEDELRQVIELIQCLHPRPGSQIESSEAEYIVPDVIVRKDNERWLVELNQEAMPRLRVN
ATYAGMVRRADSSADNTFMRNQLQEARWFIKSLQSRNETLMKVATQIVEHQRGFLDYGEEAMKPLVLHDIAEAVGMHEST
ISRVTTQKYMHTPRGIFELKYFFSSHVSTAEGGECSSTAIRAIIKKLVAAENAKKPLSDSKIAGLLEAQGIQVARRTVAK
YRESLGIAPSSERKRLV

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=201114 CEK59_RS19715 WP_023130255.1 4154217..4155710(+) (rpoN/rpoN1) [Pseudomonas aeruginosa strain LW]
ATGAAACCATCGCTAGTCCTCAAGATGGGCCAGCAGCTGACGATGACGCCGCAGCTGCAACAGGCCATACGCCTCCTCCA
GCTATCCACCCTGGACCTCCAGCAGGAAATCCAGGAAGCGCTGGAATCCAATCCCATGCTCGAACGCCAGGAAGACGGCG
ACGATTTCGACAACAGCGACCCCCTCGCCGATGGCGCCGAACAGGCGGCCAGCGCGCCCCAGGAGAGCCCTCTCCAGGAA
AGCACCACGCCCAGCGTCGAAAGCCTCGACGACGACCAGTGGAGCGAACGCATACCCAGCGAGTTGCCGGTGGATACCGC
CTGGGAAGACATCTACCAGACCAGCGCCAGCAGCCTGCCCAGCAATGACGATGACGAATGGGACTTCACCGCGCGCACCA
GCAGCGGCGAGAGCCTGCACAGCCACCTGCTGTGGCAGGTCAACCTGGCACCGATGTCGGACACCGATCGGATGATCGCG
GTGACCATCATCGATAGCATCAACAACGATGGCTACCTCGAAGAGTCCCTCGAAGAAATCCTCGCCGCCATCGATCCGGA
ACTGGACGTCGAGCTCGACGAAGTGGAAGTCGTGCTGCGCCGCATCCAGCAACTGGAACCCGCCGGCATCGGCGCCCGCA
ACCTGCGCGAGTGCCTGCTGCTGCAACTGCGCCAGTTGCCGTCCACCACGCCCTGGCTGAACGAAGCGCTGCGCCTGGTA
TCCGACTACCTCGACCTGCTCGGCGGCCGCGACTACAGCCAATTGATGCGGCGCATGAAGCTCAAGGAAGACGAACTGCG
CCAGGTCATCGAGCTGATCCAGTGCCTGCATCCCCGGCCCGGTTCGCAGATCGAATCGAGCGAAGCCGAATACATCGTCC
CCGACGTCATCGTGCGCAAGGACAACGAGCGCTGGCTGGTCGAGCTGAACCAGGAGGCGATGCCGCGCCTGCGCGTCAAC
GCCACCTACGCCGGCATGGTCCGCCGCGCCGATAGCAGCGCCGACAATACCTTCATGCGCAACCAGTTGCAGGAAGCCCG
CTGGTTCATCAAGAGCCTGCAGAGCCGCAACGAAACGCTGATGAAGGTCGCCACCCAGATCGTCGAGCACCAGCGCGGTT
TCCTCGACTACGGCGAAGAAGCCATGAAGCCGCTGGTCCTGCATGACATCGCCGAAGCGGTGGGCATGCACGAATCGACC
ATTTCGCGGGTCACCACGCAGAAATACATGCATACGCCGCGTGGCATTTTCGAGCTGAAATATTTCTTTTCAAGTCACGT
CAGTACCGCTGAAGGCGGGGAATGCTCGTCCACCGCGATCCGCGCGATCATCAAGAAACTGGTGGCCGCGGAAAATGCGA
AAAAGCCATTGAGCGACAGCAAGATCGCTGGTTTACTGGAGGCACAGGGCATTCAAGTGGCTCGCCGCACCGTCGCCAAA
TACCGCGAATCCCTGGGCATAGCCCCTTCGAGCGAGCGCAAGCGACTGGTGTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(129-323)

Sigma-54, DNA binding domain (Sigma54_DBD)

(337-495)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

46.654

100

0.477

Multiple sequence alignment