Detailed information NTG0200532

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: BJG91_RS30570
• Genome accession: NZ_CP017577
• Coordinates: 5359871..5363593 (+)
• Length: 1240 a.a.
• NCBI ID: WP_065211710.1
• Uniprot ID: -
• Organism: Bacillus thuringiensis strain SCG04-02
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 5354871..5368593

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BJG91_RS30555 (BJG91_29525)	-	5355029..5355622 (+)	594	WP_000347517.1	TVP38/TMEM64 family protein	-
BJG91_RS30560 (BJG91_29530)	lepB	5355679..5356242 (+)	564	WP_000751919.1	signal peptidase I	-
BJG91_RS30565 (BJG91_29535)	addB	5356359..5359874 (+)	3516	WP_065211709.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
BJG91_RS30570 (BJG91_29540)	addA	5359871..5363593 (+)	3723	WP_065211710.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
BJG91_RS30575 (BJG91_29545)	-	5363606..5363893 (+)	288	WP_000255728.1	RNA polymerase alpha subunit C-terminal domain-containing protein	-
BJG91_RS30580 (BJG91_29550)	gerPF	5364031..5364246 (-)	216	WP_001141566.1	spore germination protein GerPF	-
BJG91_RS30585 (BJG91_29555)	-	5364289..5364675 (-)	387	WP_000902324.1	spore germination protein GerPE	-
BJG91_RS30590 (BJG91_29560)	gerPD	5364691..5364885 (-)	195	WP_001052807.1	spore germination protein GerPD	-
BJG91_RS30595 (BJG91_29565)	gerPC	5364892..5365506 (-)	615	WP_001070760.1	spore germination protein GerPC	-
BJG91_RS30600 (BJG91_29570)	gerPB	5365574..5365780 (-)	207	WP_001012508.1	spore germination protein GerPB	-
BJG91_RS30605 (BJG91_29575)	gerPA	5365795..5366016 (-)	222	WP_001111187.1	spore germination protein GerPA	-
BJG91_RS30610 (BJG91_29580)	-	5366113..5366292 (-)	180	WP_000462841.1	aspartyl-phosphate phosphatase Spo0E family protein	-
BJG91_RS30615 (BJG91_29585)	-	5366540..5367439 (+)	900	WP_001213071.1	fumarylacetoacetate hydrolase family protein	-
BJG91_RS30620 (BJG91_29590)	-	5367476..5367757 (-)	282	WP_000926857.1	hypothetical protein	-

Sequence

Protein

• Length: 1240 a.a.
• Molecular weight: 142688.88 Da
• Isoelectric Point: 4.8010

>NTDB_id=200532 BJG91_RS30570 WP_065211710.1 5359871..5363593(+) (addA) [Bacillus thuringiensis strain SCG04-02]
MIENWPKKPEGSQWTDDQWKAVVANGRDILVAAAAGSGKTAVLVERIIKKIINEENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIDEPGSQHVRKQLSLLNKASISTIHSFCLQVIRGYYYMLDVDPRFRIANQTENELLKEEVLDDILEEEYGIE
DNTIFFELVDRYTSDRSDDDLQRMILALHTESRAHPNPEKWLDKLVEAYDVEGKTIEDLVYASYLLEDVKFQLETAEQHI
RKATELAMLPDGPAPRIETLQADVALLGTLSSAARESWTSLYEAMQNVSWQTLKRIKKSDYNEDVVKQVDSLRNKAKDEV
KKLQEELFSRKPESFLRDFQDMHPVLEKLVQLVKVFTERFQAMKRDKGMVDFTDLEHFCLQILSEQSENGEMNPSAVALQ
YRNKFAEVLVDEYQDTNFVQESIIKFVTKDSESEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGLGGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIDYDADAELKLGATYPEGEDVAAELLCIQQTEEVIDGEEGAEIEKAQLEARLMA
QRIKAMVDSGYEVYDRKNDSMRPVQYRDFVILLRSMPWAPQIMEELKLQGIPVYADLATGYFEATEVNIMMNVFRVIDNP
MQDIPLAAVLRSPIVGLSDEELATLRAHGKKGSFYEVMSSFLKGAPLEEEQELHDKLEWFYNLLQGWREFARQQSLSDLI
WKVYGETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTIH
KSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALTRA
KEKLILIGTVKDANKEMEKWLDAREHSEWLLPDHIRAGASCYLDWIAPSLYRHRDSEMLLELGQGSIPDEIYGYDTSWKV
EVVDGNTLLAPEPVQEEKQELLEALREKKAVPLQSERKEEVYDRLMWKYGYEDATSHRAKQSVTEIKRNYQSEEGSDNAF
IKKLRAPIKTRPRFMEKKGLTYAERGTAVHAVMQHVDLKKPITVEVLQEQIAGMVNKELLTFEQAEEIAIEKVISFFDSD
LGKRVLAAKSVEREVPFTMMLAAEEAYQDWQGKSEETILVQGVIDCMIEEEDGITLIDFKTDTIEEKFPGGFEQAKPILE
DRYKVQLSLYAKALEKSLQHPVKEKCLYFFDGNHVVNIEE

Nucleotide

• Length: 3723 bp

>NTDB_id=200532 BJG91_RS30570 WP_065211710.1 5359871..5363593(+) (addA) [Bacillus thuringiensis strain SCG04-02]
ATGATAGAAAATTGGCCTAAAAAACCAGAAGGTAGTCAATGGACAGATGACCAGTGGAAAGCTGTTGTAGCTAACGGACG
TGATATTTTAGTTGCTGCTGCAGCTGGATCAGGTAAAACAGCAGTATTAGTTGAACGTATTATTAAAAAGATTATTAATG
AAGAGAACCCAGTCGATGTCGACCGCCTGCTCGTTGTAACATTTACGAATGCAGCAGCACAAGAGATGAAAAACCGAATC
GGAGAAGCGTTAGAAAAGGTATTAATTGATGAACCAGGCTCTCAACACGTAAGAAAGCAGCTGAGCCTATTAAATAAAGC
TTCCATTTCAACAATTCACTCATTCTGTTTACAAGTAATTAGAGGATATTACTACATGCTTGATGTTGATCCTCGTTTCC
GTATTGCGAATCAAACAGAAAATGAATTGTTAAAAGAAGAAGTGCTAGATGACATATTAGAAGAAGAGTATGGCATCGAA
GATAATACGATATTCTTTGAACTCGTTGATCGTTATACGAGTGACCGTAGTGATGATGACTTACAACGTATGATTTTAGC
GCTTCATACAGAATCAAGAGCGCATCCAAATCCGGAAAAATGGCTTGATAAATTAGTAGAAGCATATGACGTAGAAGGAA
AGACAATTGAGGATTTAGTATATGCTTCTTATTTATTAGAAGATGTGAAATTCCAGCTTGAAACAGCGGAACAGCATATT
CGTAAGGCAACTGAACTCGCAATGCTTCCTGACGGTCCAGCGCCTCGCATTGAAACCCTGCAAGCAGATGTAGCTTTACT
TGGAACGCTATCATCAGCTGCTCGTGAGTCGTGGACAAGTTTGTATGAAGCGATGCAAAACGTATCGTGGCAAACGTTAA
AGCGTATTAAGAAAAGTGATTACAATGAAGATGTTGTAAAACAAGTAGACTCTCTTCGTAATAAAGCGAAAGATGAAGTG
AAGAAATTACAAGAAGAGTTATTTAGCCGCAAACCTGAAAGTTTCTTACGAGATTTTCAAGATATGCACCCAGTATTAGA
AAAGCTTGTTCAACTTGTAAAAGTATTTACAGAGCGTTTCCAAGCGATGAAGCGAGATAAAGGAATGGTCGATTTCACAG
ATTTAGAGCATTTCTGTTTACAAATTTTAAGTGAACAAAGTGAAAATGGTGAAATGAATCCGTCAGCAGTGGCGCTCCAA
TATCGTAATAAATTTGCTGAAGTATTAGTCGATGAATATCAAGACACGAACTTTGTACAGGAATCCATTATTAAATTCGT
AACGAAAGATTCTGAGAGTGAAGGAAACTTGTTCATGGTTGGTGACGTAAAGCAGTCGATTTATCGTTTCCGACTAGCCG
AGCCAGGCTTATTCCTAGGAAAGTATAAACGTTTCACACAAGAAGGATTGGGCGGCGGAATGAAGATTGACTTAGCGAAA
AACTTCCGTAGTCGTCATGAAGTACTAGCAGGTACGAACTTTATTTTTAAACAAATTATGGGCGAAGAAGTTGGGGAAAT
TGACTACGATGCTGACGCTGAACTAAAGCTAGGTGCTACCTATCCAGAAGGTGAAGATGTAGCAGCAGAATTATTATGTA
TTCAGCAAACGGAAGAAGTAATAGACGGTGAAGAAGGTGCAGAAATCGAAAAAGCACAGCTTGAAGCTCGCCTTATGGCG
CAGCGCATTAAAGCGATGGTTGATTCCGGTTATGAAGTGTATGACCGTAAAAATGATAGTATGCGTCCGGTGCAATATCG
CGATTTCGTTATTTTACTTCGCTCCATGCCGTGGGCCCCGCAAATTATGGAAGAGTTAAAATTACAAGGAATTCCAGTAT
ACGCTGACCTCGCGACTGGTTATTTTGAAGCGACGGAAGTAAATATTATGATGAACGTATTCCGCGTTATTGATAATCCG
ATGCAAGATATTCCGCTTGCCGCTGTACTTCGTTCCCCAATCGTTGGATTGAGCGATGAAGAACTTGCAACGCTTCGTGC
TCATGGAAAGAAAGGCTCGTTTTATGAAGTAATGAGCTCATTCTTAAAAGGGGCACCGCTTGAAGAGGAGCAAGAACTTC
ATGATAAATTAGAGTGGTTTTATAACTTACTGCAAGGATGGCGTGAATTTGCCCGTCAACAATCCCTTTCTGATTTAATT
TGGAAAGTGTACGGTGAGACAGGTTATTACGACTTCGTTGGCGGTTTACCAGCAGGAAAGCAAAGACAAGCAAACTTACG
CGTACTATATGACCGTGCAAGACAATATGAAGCAACATCATTTAGAGGGTTATTCCGCTTCTTACGTTTTATTGAACGTA
TTTTAGAACGCGGTGATGATATGGGTACGGCGAGAGCCCTAGGTGAACAAGAAGACGTTGTTCGCATTATGACGATTCAT
AAAAGTAAAGGGCTAGAGTTCCCGGTCGTATTTGTAGCTGGACTCGGTCGTCGTTTTAATACACAAGACTTAATGAAACG
TTTCTTATTGCATAAAGATTTCGGTTTCGGTTCGCAATTTATCGATCCGCGTAAACGAATTAAATATACAACATTATCAC
AGCTTGCGATTAAGCGTAAAATGAAAATGGAATTAATTGCGGAAGAAATGCGCGTACTATACGTAGCATTGACGCGTGCG
AAAGAGAAGTTAATTTTAATCGGAACAGTTAAGGATGCAAATAAGGAAATGGAAAAATGGCTTGATGCGAGGGAACATAG
TGAATGGTTATTACCAGATCACATACGTGCCGGAGCGTCTTGTTATTTAGACTGGATTGCACCTTCATTATATAGACACC
GTGATAGTGAAATGCTTCTTGAATTAGGACAAGGAAGTATTCCAGATGAAATTTACGGATATGACACTAGCTGGAAAGTA
GAAGTTGTGGACGGTAACACACTACTTGCACCAGAGCCAGTTCAAGAAGAGAAACAAGAATTGTTAGAAGCACTTCGTGA
GAAAAAGGCTGTTCCCCTGCAAAGTGAACGGAAAGAAGAAGTGTACGACAGATTAATGTGGAAGTACGGATATGAGGATG
CGACATCTCATCGTGCGAAGCAATCTGTTACAGAAATAAAGAGAAATTATCAATCTGAAGAAGGTAGCGATAACGCCTTT
ATTAAAAAACTACGTGCACCAATTAAAACACGCCCGCGCTTTATGGAGAAAAAAGGGTTAACGTACGCAGAGCGCGGAAC
AGCAGTCCATGCCGTTATGCAGCATGTTGATTTGAAGAAGCCGATTACGGTTGAAGTTCTTCAAGAGCAAATTGCTGGAA
TGGTAAATAAGGAATTATTAACATTCGAGCAGGCGGAAGAAATAGCGATTGAAAAAGTAATTTCATTCTTTGACAGTGAC
TTAGGTAAAAGGGTATTAGCGGCGAAAAGTGTTGAGCGTGAAGTGCCATTTACGATGATGCTTGCAGCAGAAGAAGCATA
TCAAGATTGGCAAGGGAAGAGTGAAGAAACGATTCTTGTCCAAGGGGTTATCGACTGCATGATTGAAGAGGAAGATGGCA
TTACGTTAATCGACTTTAAAACAGATACGATTGAAGAGAAATTCCCAGGCGGATTCGAACAAGCGAAACCAATTTTAGAA
GATCGATATAAAGTGCAGCTTTCGTTATATGCAAAAGCACTGGAGAAAAGCTTACAACATCCTGTAAAAGAGAAATGTTT
ATACTTCTTTGATGGGAATCACGTTGTAAATATTGAAGAATAG

Domains

Predicted by InterproScan.

• UvrD-like helicase C-terminal domain (UvrD_C) (476-889)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (14-465)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1059-1230)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	53.291	100	0.535