Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   DCWBC2_RS00280 Genome accession   NZ_CP017572
Coordinates   54076..55470 (+) Length   464 a.a.
NCBI ID   WP_034376942.1    Uniprot ID   A0A142V8C5
Organism   Dehalococcoides mccartyi isolate WBC-2     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 49076..60470
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DCWBC2_RS00275 (DCWBC2_0053) - 51155..53629 (+) 2475 WP_011308690.1 ATP-dependent Clp protease ATP-binding subunit -
  DCWBC2_RS00280 (DCWBC2_0054) radA 54076..55470 (+) 1395 WP_034376942.1 DNA repair protein RadA Machinery gene
  DCWBC2_RS00285 (DCWBC2_0055) - 55457..56140 (+) 684 WP_011308692.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  DCWBC2_RS00290 (DCWBC2_0056) ispF 56146..56619 (+) 474 WP_011308693.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  DCWBC2_RS00295 (DCWBC2_0057) cysS 56623..57996 (+) 1374 WP_011308694.1 cysteine--tRNA ligase -
  DCWBC2_RS00305 (DCWBC2_0059) - 58214..59212 (+) 999 WP_135350353.1 tyrosine-type recombinase/integrase -
  DCWBC2_RS00310 - 59595..60020 (+) 426 WP_119194311.1 hypothetical protein -

Sequence

Protein


Download         Length: 464 a.a.        Molecular weight: 49403.74 Da        Isoelectric Point: 6.5004

>NTDB_id=200473 DCWBC2_RS00280 WP_034376942.1 54076..55470(+) (radA) [Dehalococcoides mccartyi isolate WBC-2]
MDKSRNVYICSNCGHESLKWLGRCPGCQEWNTLEETTIAAPLGRKNAPARVISPAAELSSLNASDTTRRSLSISEFNRVL
GGGIVPGSLMLLGGEPGIGKSTLLLQVAASVAQSGGKVVYVSGEENPAQIKMRAQRLGISGEGLFLMAETDLNAILAQLS
VLCPSLVVIDSIQTVFLPELEAAPGAINQVRESALRLMQWAKNSGASVFIAAHVTKEGNIAGPRILEHIVDVVMYFEGES
QSAYRLIRSVKNRFGSTNEVGIFEMKSEGLVEVANPSQIFLSNRQANTVGSAVTAVLEGSRPLLVEVQALTNTTSFGQPR
RTANGVDFNRTIMIAAVLSKRLSMRLGTQDIIVNATGGIRLDEPAADLAIALAIASSYRDIGVCPETIALGEIGLSGELR
TIPHLERRLSEASRLGFTRALVPAGANCQNININGIQIIAVSTVKEAIKLALTGVKTETEDVFE

Nucleotide


Download         Length: 1395 bp        

>NTDB_id=200473 DCWBC2_RS00280 WP_034376942.1 54076..55470(+) (radA) [Dehalococcoides mccartyi isolate WBC-2]
ATGGATAAAAGCCGTAATGTTTATATCTGTTCCAACTGCGGACATGAGAGCCTGAAGTGGCTGGGGCGTTGCCCCGGCTG
TCAGGAATGGAATACTCTTGAAGAAACAACAATTGCTGCACCTCTCGGGCGTAAAAATGCACCTGCCCGGGTTATCAGCC
CGGCAGCCGAGCTTTCCAGTCTGAATGCATCAGATACTACCCGCCGGAGTCTATCTATTTCAGAGTTCAACCGGGTTTTG
GGCGGTGGAATAGTGCCGGGTTCACTGATGCTTTTAGGGGGTGAACCGGGTATCGGCAAATCCACGCTCCTTCTTCAGGT
GGCTGCCTCGGTAGCCCAGAGCGGCGGCAAAGTGGTATATGTTTCCGGGGAAGAGAACCCCGCCCAGATAAAAATGCGCG
CTCAGCGTCTGGGTATCAGCGGCGAGGGGCTTTTCCTTATGGCTGAGACAGACCTTAATGCCATTCTAGCCCAGCTTTCA
GTCCTCTGCCCGTCATTGGTTGTTATAGACTCTATCCAGACTGTATTCCTGCCTGAGCTTGAGGCCGCACCCGGTGCTAT
AAATCAGGTGCGTGAATCAGCCCTCCGCCTGATGCAGTGGGCTAAGAACAGCGGTGCCAGCGTATTTATTGCCGCCCATG
TTACCAAAGAGGGCAACATCGCCGGGCCGCGCATACTGGAACATATAGTAGATGTGGTCATGTACTTTGAGGGCGAGTCC
CAGAGTGCTTACCGTTTGATACGTTCGGTCAAAAACCGCTTCGGTTCTACCAACGAAGTAGGCATATTTGAAATGAAAAG
TGAAGGATTGGTGGAAGTAGCCAATCCCTCGCAGATATTTTTATCTAATCGGCAGGCAAACACTGTTGGTTCAGCTGTAA
CAGCAGTGCTGGAGGGTAGCCGCCCTTTACTGGTAGAGGTGCAGGCACTCACCAATACCACCAGTTTCGGCCAGCCGCGC
CGCACGGCCAACGGGGTAGATTTTAACCGTACTATTATGATAGCCGCTGTTCTTTCCAAACGCCTTTCCATGCGGCTGGG
TACCCAGGACATAATAGTAAATGCCACCGGCGGTATTCGTCTGGACGAGCCGGCCGCAGATTTGGCTATTGCTTTGGCCA
TTGCCTCCAGTTACCGTGATATCGGGGTCTGCCCGGAAACCATAGCACTGGGTGAGATTGGCCTTTCAGGGGAACTGAGG
ACAATCCCTCATTTGGAAAGACGTCTTTCCGAGGCCAGCCGTTTGGGTTTTACGAGGGCTTTGGTACCTGCCGGTGCTAA
TTGCCAGAATATAAATATAAATGGTATCCAGATTATTGCGGTTTCAACTGTCAAAGAGGCTATTAAACTGGCGCTTACCG
GGGTAAAAACGGAGACCGAAGATGTTTTTGAATGA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(335-424)

AAA domain (AAA_25)

(66-218)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A142V8C5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

50.218

98.707

0.496
  radA Streptococcus pneumoniae Rx1

48.578

98.491

0.478
  radA Streptococcus pneumoniae D39

48.578

98.491

0.478
  radA Streptococcus pneumoniae R6

48.578

98.491

0.478
  radA Streptococcus pneumoniae TIGR4

48.578

98.491

0.478
  radA Streptococcus mitis SK321

48.578

98.491

0.478
  radA Streptococcus mitis NCTC 12261

48.359

98.491

0.476

Multiple sequence alignment