Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   A4W85_RS08735 Genome accession   NZ_CP017570
Coordinates   1720520..1721884 (-) Length   454 a.a.
NCBI ID   WP_011375373.1    Uniprot ID   A0A094XXJ4
Organism   Latilactobacillus sakei strain TMW 1.578     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1715520..1726884
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A4W85_RS08710 (A4W85_08675) - 1715726..1716258 (-) 533 Protein_1678 NYN domain-containing protein -
  A4W85_RS08715 (A4W85_08680) rlmB 1716262..1717149 (-) 888 WP_082267809.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  A4W85_RS08720 (A4W85_08685) - 1717136..1717543 (-) 408 WP_251944918.1 Mini-ribonuclease 3 -
  A4W85_RS08725 (A4W85_08690) cysS 1717540..1718946 (-) 1407 WP_251944934.1 cysteine--tRNA ligase -
  A4W85_RS08730 (A4W85_08695) - 1719377..1720489 (-) 1113 WP_011375372.1 PIN/TRAM domain-containing protein -
  A4W85_RS08735 (A4W85_08700) radA 1720520..1721884 (-) 1365 WP_011375373.1 DNA repair protein RadA Machinery gene
  A4W85_RS08740 (A4W85_08705) - 1721957..1722496 (-) 540 WP_011375374.1 dUTP diphosphatase -
  A4W85_RS08745 (A4W85_08710) rpiA 1722692..1723375 (+) 684 WP_011375375.1 ribose-5-phosphate isomerase RpiA -
  A4W85_RS08750 (A4W85_08715) - 1723850..1725172 (+) 1323 WP_251944937.1 C1 family peptidase -
  A4W85_RS08755 (A4W85_08720) - 1725377..1726723 (+) 1347 WP_076632142.1 C1 family peptidase -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 49598.97 Da        Isoelectric Point: 7.3488

>NTDB_id=200469 A4W85_RS08735 WP_011375373.1 1720520..1721884(-) (radA) [Latilactobacillus sakei strain TMW 1.578]
MAKVKTQFVCQNCGYSSPRFLGRCPNCGAWNQMVEEREQPAAAAKSNFTISGRATEPEKISTVNIQKEPRVKTELNELNR
VLGGGVVPGSLILIGGDPGIGKSTLLLQVSGQLEKVGKILYVSGEESASQIKMRANRLGVNGDQLYLYPETDMGNIRHQI
ETLKPEYVVIDSIQTMSEPEVTSAVGSVSQVRQVTAELMRIAKTNQITIFVVGHVTKEGAIAGPKILEHMVDTVLYFEGD
THHTYRILRSVKNRFGSTNEIGIFEMREAGLQEVANPSEIFLEERLAGATGSAVVVSMEGTRPILVELQTLITPTLFGNA
KRTSSGLDHNRVSLIMAVLEKRASLMLSNQDAYLKATGGVKLDEPAIDLAMAVSIASSYRDKEIPPTDCFVGEIGLTGEI
RRVNRIEQRVGEAAKLGFKRIYVPKNNLQGWDPPTDIQVVGVTTIAETLKKVFN

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=200469 A4W85_RS08735 WP_011375373.1 1720520..1721884(-) (radA) [Latilactobacillus sakei strain TMW 1.578]
GTGGCTAAAGTTAAAACGCAGTTTGTCTGCCAGAATTGTGGCTATAGTTCCCCCAGATTCTTGGGGCGCTGTCCGAATTG
TGGTGCTTGGAACCAAATGGTTGAAGAACGTGAACAACCAGCCGCAGCGGCAAAATCAAATTTCACAATTTCAGGGCGCG
CCACTGAACCTGAAAAAATTAGTACCGTTAATATCCAAAAAGAACCCCGTGTTAAAACGGAATTAAACGAATTGAATCGT
GTTTTAGGTGGCGGCGTTGTGCCGGGGTCACTGATTTTAATCGGTGGGGATCCTGGGATTGGGAAATCAACCTTGTTACT
ACAAGTTTCAGGGCAACTTGAAAAAGTGGGTAAGATCCTATACGTTTCAGGTGAAGAAAGTGCCTCGCAAATTAAGATGC
GGGCGAATCGATTAGGAGTTAATGGCGATCAATTGTATCTGTATCCCGAAACGGATATGGGCAATATTCGTCACCAAATT
GAAACGCTCAAACCGGAATACGTCGTGATTGATTCGATTCAAACGATGAGTGAACCAGAAGTGACTTCCGCAGTCGGGAG
TGTTTCGCAAGTTCGCCAAGTAACCGCAGAATTGATGCGGATTGCTAAAACGAATCAAATTACCATCTTCGTGGTAGGAC
ATGTGACGAAGGAAGGGGCGATTGCCGGCCCTAAGATTTTGGAACATATGGTAGATACCGTGTTGTATTTTGAAGGTGAC
ACCCATCACACTTACCGGATTTTACGATCTGTTAAAAACCGGTTTGGTTCAACTAATGAAATCGGGATTTTCGAAATGCG
GGAAGCGGGCTTGCAAGAAGTCGCTAATCCATCCGAAATTTTCCTTGAAGAACGACTGGCCGGTGCGACTGGTTCAGCAG
TTGTGGTTTCAATGGAAGGGACAAGACCAATCTTGGTTGAGTTACAAACTTTGATTACCCCAACCTTGTTCGGGAATGCG
AAACGGACCTCTTCAGGGTTAGATCATAATCGGGTGTCATTGATTATGGCGGTTTTAGAAAAGCGGGCGAGTTTGATGCT
TTCAAACCAAGATGCTTATCTTAAAGCAACCGGTGGCGTTAAGTTAGATGAACCAGCGATTGATTTGGCGATGGCCGTTT
CAATTGCTTCCAGTTATCGCGATAAAGAAATTCCACCAACCGATTGTTTTGTCGGCGAAATTGGCTTGACGGGTGAAATC
CGGCGGGTCAACCGGATCGAACAACGCGTTGGCGAAGCGGCGAAGTTAGGTTTTAAACGGATTTATGTTCCTAAAAATAA
TTTGCAAGGTTGGGATCCACCAACTGATATTCAAGTCGTTGGGGTCACCACTATTGCAGAAACTTTAAAAAAAGTATTCA
ACTAG

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(363-427)

AAA domain (AAA_25)

(70-219)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A094XXJ4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

69.316

99.78

0.692
  radA Streptococcus mitis SK321

69.316

99.78

0.692
  radA Streptococcus pneumoniae Rx1

69.095

99.78

0.689
  radA Streptococcus pneumoniae D39

69.095

99.78

0.689
  radA Streptococcus pneumoniae R6

69.095

99.78

0.689
  radA Streptococcus pneumoniae TIGR4

69.095

99.78

0.689
  radA Bacillus subtilis subsp. subtilis str. 168

65.121

99.78

0.65

Multiple sequence alignment