Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   A4W84_RS06590 Genome accession   NZ_CP017568
Coordinates   1299397..1299909 (-) Length   170 a.a.
NCBI ID   WP_035145013.1    Uniprot ID   A0A9N7J0G1
Organism   Latilactobacillus sakei strain TMW 1.417     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1294397..1304909
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A4W84_RS06560 (A4W84_06455) - 1295323..1295688 (+) 366 WP_011374991.1 DUF805 domain-containing protein -
  A4W84_RS06570 (A4W84_06465) - 1296231..1296689 (-) 459 WP_011374992.1 hypothetical protein -
  A4W84_RS06575 (A4W84_06470) - 1296744..1297940 (-) 1197 WP_011374993.1 acetate/propionate family kinase -
  A4W84_RS06580 (A4W84_06475) - 1297962..1298972 (-) 1011 WP_011374994.1 class I SAM-dependent methyltransferase -
  A4W84_RS06585 (A4W84_06480) - 1299096..1299434 (-) 339 WP_035145011.1 hypothetical protein -
  A4W84_RS06590 (A4W84_06485) comGF 1299397..1299909 (-) 513 WP_035145013.1 competence type IV pilus minor pilin ComGF Machinery gene
  A4W84_RS06595 (A4W84_06490) comGE 1299884..1300189 (-) 306 WP_011374997.1 hypothetical protein Machinery gene
  A4W84_RS06600 (A4W84_06495) comGD 1300176..1300628 (-) 453 WP_056947126.1 competence type IV pilus minor pilin ComGD Machinery gene
  A4W84_RS06605 (A4W84_06500) comGC 1300600..1300899 (-) 300 WP_056947129.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  A4W84_RS06610 (A4W84_06505) comGB 1300896..1301903 (-) 1008 WP_056947133.1 type II secretion system F family protein Machinery gene
  A4W84_RS06615 (A4W84_06510) comGA 1301896..1302786 (-) 891 WP_099947399.1 competence type IV pilus ATPase ComGA Machinery gene
  A4W84_RS06620 (A4W84_06515) - 1302902..1303633 (-) 732 WP_076632059.1 YebC/PmpR family DNA-binding transcriptional regulator -
  A4W84_RS06625 (A4W84_06520) - 1303724..1304224 (-) 501 WP_076632058.1 VanZ family protein -
  A4W84_RS06630 (A4W84_06525) - 1304321..1304695 (+) 375 WP_099947400.1 hypothetical protein -

Sequence

Protein


Download         Length: 170 a.a.        Molecular weight: 19689.58 Da        Isoelectric Point: 6.9899

>NTDB_id=200423 A4W84_RS06590 WP_035145013.1 1299397..1299909(-) (comGF) [Latilactobacillus sakei strain TMW 1.417]
MRLIGRPIKHSAFTLIEMVISMAILILTSQLLVATVGVLQKAIKVTQQQQNTINWHISTIQLDQFLADAYFTHHQDQDGS
KITFRKPDTNQSGEEAETVYYLKRMKNEMVVVSQLNGYMPLLGNLKQLQLDYQRPFVQIDATFTDQSRYQHEVFLEEQDE
QAKKKTIKTD

Nucleotide


Download         Length: 513 bp        

>NTDB_id=200423 A4W84_RS06590 WP_035145013.1 1299397..1299909(-) (comGF) [Latilactobacillus sakei strain TMW 1.417]
TTGCGATTAATTGGACGACCGATTAAGCATTCAGCGTTCACGCTAATTGAAATGGTGATTTCAATGGCGATTCTAATCCT
GACGAGTCAGTTACTGGTGGCAACGGTGGGTGTTTTACAAAAGGCAATTAAAGTCACGCAACAACAGCAAAATACAATCA
ATTGGCATATCAGTACGATTCAGTTAGATCAGTTTTTAGCCGATGCGTATTTTACACATCATCAGGATCAAGATGGCTCC
AAAATCACTTTTCGAAAACCAGACACTAATCAGTCAGGGGAGGAAGCGGAGACTGTTTACTATTTAAAAAGGATGAAAAA
TGAAATGGTTGTTGTCAGCCAATTAAATGGGTATATGCCATTATTAGGCAACCTCAAGCAACTTCAACTTGACTACCAGC
GACCGTTTGTTCAAATCGATGCGACTTTTACGGATCAGAGTCGCTACCAACACGAAGTTTTCTTGGAGGAACAAGATGAA
CAGGCTAAAAAAAAGACGATTAAAACCGATTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(40-149)

Prokaryotic N-terminal methylation motif (N_methyl)

(9-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Latilactobacillus sakei subsp. sakei 23K

98.235

100

0.982

Multiple sequence alignment