Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   A4W84_RS05460 Genome accession   NZ_CP017568
Coordinates   1077252..1077959 (-) Length   235 a.a.
NCBI ID   WP_231952379.1    Uniprot ID   -
Organism   Latilactobacillus sakei strain TMW 1.417     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1072252..1082959
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A4W84_RS05435 (A4W84_05350) rpsO 1072546..1072815 (-) 270 WP_011374766.1 30S ribosomal protein S15 -
  A4W84_RS05440 (A4W84_05355) rpsT 1073094..1073348 (+) 255 WP_011374767.1 30S ribosomal protein S20 -
  A4W84_RS05445 (A4W84_05360) holA 1073417..1074445 (-) 1029 WP_011374768.1 DNA polymerase III subunit delta -
  A4W84_RS05450 (A4W84_05365) comEC 1074442..1076709 (-) 2268 WP_251908015.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  A4W84_RS05455 (A4W84_05370) - 1076706..1077194 (-) 489 WP_011374770.1 ComE operon protein 2 -
  A4W84_RS05460 (A4W84_05375) comEA 1077252..1077959 (-) 708 WP_231952379.1 helix-hairpin-helix domain-containing protein Machinery gene
  A4W84_RS05465 (A4W84_05380) - 1078031..1079077 (-) 1047 WP_251908017.1 SepM family pheromone-processing serine protease -
  A4W84_RS05470 (A4W84_05385) coaD 1079079..1079576 (-) 498 WP_251908018.1 pantetheine-phosphate adenylyltransferase -
  A4W84_RS05475 (A4W84_05390) rsmD 1079589..1080143 (-) 555 WP_011374774.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  A4W84_RS05480 (A4W84_05395) - 1080148..1080438 (-) 291 WP_011374775.1 YlbG family protein -
  A4W84_RS05485 (A4W84_05400) - 1080462..1081550 (-) 1089 WP_250824293.1 CAP-associated domain-containing protein -
  A4W84_RS05490 (A4W84_05405) - 1081561..1082748 (-) 1188 WP_011374777.1 FtsW/RodA/SpoVE family cell cycle protein -

Sequence

Protein


Download         Length: 235 a.a.        Molecular weight: 26146.73 Da        Isoelectric Point: 9.7682

>NTDB_id=200419 A4W84_RS05460 WP_231952379.1 1077252..1077959(-) (comEA) [Latilactobacillus sakei strain TMW 1.417]
MMWQDYWQRYRRFWWVPCLVLLGIIAGGFYWHQSQQKQKVTQNEQLLATSASKTKPATKKTHQAATKKKGGFVDIKGAVV
YPGIYPITVSETRLFEVLKNAGGPTDAADTKRLNLAQKLTDQLIIYIPKKGEVVTDQELIQAPQQIATTSERTQEQASTG
TSANASSTSDTAKIDLNQADSTQLQTLNGIGPKKAEQIIAYRDEQGKFKQIEDLQKVGGIGPKTFEQLQSQICVN

Nucleotide


Download         Length: 708 bp        

>NTDB_id=200419 A4W84_RS05460 WP_231952379.1 1077252..1077959(-) (comEA) [Latilactobacillus sakei strain TMW 1.417]
ATGATGTGGCAAGATTATTGGCAGCGATATCGGCGTTTTTGGTGGGTGCCTTGCTTAGTGCTATTGGGGATCATCGCGGG
TGGCTTTTATTGGCATCAATCGCAACAAAAACAAAAAGTAACGCAAAATGAGCAACTGCTAGCAACCAGTGCGTCTAAAA
CAAAACCCGCTACTAAGAAGACACACCAAGCGGCAACCAAGAAAAAAGGCGGTTTTGTCGATATAAAAGGCGCGGTGGTC
TACCCGGGAATTTATCCGATCACAGTCTCTGAAACGCGCTTATTTGAGGTGCTCAAAAATGCAGGTGGTCCGACTGATGC
GGCTGATACCAAGCGGCTTAATTTAGCTCAAAAGTTAACCGATCAACTGATTATTTATATTCCGAAAAAAGGAGAGGTGG
TTACTGATCAAGAACTCATTCAAGCGCCACAACAAATCGCAACGACCAGTGAGCGTACGCAAGAACAGGCATCAACTGGG
ACAAGTGCGAATGCATCGAGCACGAGTGACACTGCTAAAATCGATTTGAATCAGGCTGATAGTACGCAGCTTCAGACCCT
CAATGGCATTGGCCCCAAGAAAGCTGAACAAATAATTGCTTATCGTGATGAACAAGGAAAATTTAAACAGATTGAAGATC
TACAAAAAGTCGGCGGGATTGGTCCGAAGACGTTTGAACAATTGCAATCACAAATTTGTGTGAATTAG

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(73-124)

Helix-hairpin-helix motif (HHH_3)

(172-233)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Latilactobacillus sakei subsp. sakei 23K

100

100

1
  comEA Lactococcus lactis subsp. cremoris KW2

36.797

98.298

0.362

Multiple sequence alignment