Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   BJG93_RS01660 Genome accession   NZ_CP017561
Coordinates   339586..341103 (-) Length   505 a.a.
NCBI ID   WP_027199629.1    Uniprot ID   A0A1I9YHT2
Organism   Paraburkholderia sprentiae WSM5005     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 334586..346103
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BJG93_RS01635 (BJG93_11030) - 335485..336120 (-) 636 WP_027199626.1 LON peptidase substrate-binding domain-containing protein -
  BJG93_RS01640 (BJG93_11025) rapZ 336163..337056 (-) 894 WP_027199627.1 RNase adapter RapZ -
  BJG93_RS01645 (BJG93_11020) hprK 337155..338123 (-) 969 WP_008921706.1 HPr(Ser) kinase/phosphatase -
  BJG93_RS01650 (BJG93_11015) - 338203..338718 (-) 516 WP_083421008.1 PTS sugar transporter subunit IIA -
  BJG93_RS01655 (BJG93_11010) hpf 339060..339416 (-) 357 WP_027199628.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  BJG93_RS01660 (BJG93_11005) rpoN/rpoN1 339586..341103 (-) 1518 WP_027199629.1 RNA polymerase factor sigma-54 Machinery gene
  BJG93_RS01665 (BJG93_11000) lptB 341298..342074 (-) 777 WP_154671894.1 LPS export ABC transporter ATP-binding protein -
  BJG93_RS01670 (BJG93_10995) lptA 342092..342763 (-) 672 WP_027199631.1 lipopolysaccharide transport periplasmic protein LptA -
  BJG93_RS01675 (BJG93_10990) lptC 342802..343389 (-) 588 WP_027199632.1 LPS export ABC transporter periplasmic protein LptC -
  BJG93_RS01680 (BJG93_10985) - 343386..343946 (-) 561 WP_027199633.1 HAD family hydrolase -
  BJG93_RS01685 (BJG93_10980) kdsD 343946..344929 (-) 984 WP_034479831.1 arabinose 5-phosphate isomerase KdsD -

Sequence

Protein


Download         Length: 505 a.a.        Molecular weight: 55320.49 Da        Isoelectric Point: 4.9404

>NTDB_id=200351 BJG93_RS01660 WP_027199629.1 339586..341103(-) (rpoN/rpoN1) [Paraburkholderia sprentiae WSM5005]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAISQNPLLENEDEWIASPLRVAADGSVIAQTPGSSGPADPM
AGNGSTSSEGNGERAESGEPQGVDEYNGLSGDGGGDTSQWNLDDYGRSGNASDDDDLPPLQIHESSTSLRDHLMAQLRVT
QASQRDRALVTFLIESLDDDGYLAATLEEVLADLPEELEVDLDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSP
TRTLALDIVGHYLELLAARDFTRLRKHLKACDDDLRDAHLLIRSLEPFPGAAYGKAEADYVVPDIMVRKTAQGWQAELNP
EVVPKLRINHLYANILRNNRGDLGSGSLRQQLQEARWLIKNIQQRFETILRVAQAIVERQKSFFVHGEIAMRPLVLREIA
DTLGLHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAENPKSPLSDSRIAELLAEQGF
VVARRTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1518 bp        

>NTDB_id=200351 BJG93_RS01660 WP_027199629.1 339586..341103(-) (rpoN/rpoN1) [Paraburkholderia sprentiae WSM5005]
ATGAAAGCCAGCCTCCAACTCCGCCTATCGCAGCATCTTGCGCTGACGCCGCAACTGCAACAGTCCATCCGGCTGCTTCA
GTTGTCGACGCTCGAACTGCAGCAGGAAGTCGCCATGGCGATCTCGCAGAATCCGCTCCTCGAAAACGAAGATGAATGGA
TCGCGAGCCCGTTGCGGGTGGCAGCCGATGGTTCCGTGATCGCCCAGACGCCGGGTTCGTCGGGGCCGGCCGATCCGATG
GCCGGAAACGGTTCAACATCGTCCGAGGGCAACGGCGAGCGCGCCGAAAGCGGCGAGCCACAGGGTGTCGACGAATACAA
CGGCCTGTCGGGCGACGGCGGCGGCGATACGTCGCAGTGGAACCTCGACGACTACGGCCGCTCTGGCAACGCATCGGACG
ACGACGACCTGCCCCCGCTGCAGATCCACGAATCGAGCACGTCGCTGCGCGACCACCTGATGGCGCAACTGCGCGTCACC
CAGGCGAGCCAGCGCGACCGCGCGCTCGTCACGTTCCTGATCGAATCGCTGGACGACGACGGCTATCTGGCCGCCACCCT
CGAAGAAGTGCTGGCCGATCTGCCCGAAGAGCTCGAAGTCGACCTCGACGAATTGAACGCCGCGCTTGCGCTGCTGCATA
GCTTCGACCCGGCCGGCGTCGGCGCGCGGTCGGCGTCGGAATGCCTGAAGCTGCAGTTGTTGCGGCTCGATCCATCGCCC
ACGCGCACACTCGCGCTAGACATTGTCGGCCACTACCTGGAACTGCTCGCAGCGCGCGATTTCACGCGGCTGCGAAAACA
TCTGAAGGCCTGCGACGACGATCTGCGCGACGCGCATCTACTGATCCGCTCGCTCGAACCGTTTCCGGGGGCCGCCTACG
GCAAAGCCGAAGCCGACTACGTGGTGCCGGACATCATGGTGCGCAAAACGGCACAGGGCTGGCAGGCGGAGCTCAATCCG
GAGGTGGTGCCGAAGCTGCGGATTAACCATTTATACGCGAACATTCTGCGCAATAACCGCGGCGATCTCGGCAGCGGATC
GTTGCGCCAGCAACTGCAGGAAGCACGCTGGCTGATTAAAAACATCCAGCAGCGTTTCGAGACGATCCTGCGAGTTGCAC
AGGCAATTGTCGAGCGTCAGAAGAGCTTTTTTGTGCACGGCGAAATCGCCATGCGCCCCTTGGTTTTGCGGGAAATTGCT
GATACGCTGGGCCTACACGAGTCGACTGTCTCGCGTGTGACAACCGGTAAATACATGCTGACCCCATTCGGGACGCTGGA
ATTCAAGTACTTTTTCGGCTCACACGTTTCGACGGACACCGGCGGCGCAGCCTCGTCCACGGCCATCCGCGCGCTGATCA
AACAACTGATAGGAGCGGAAAACCCCAAATCGCCACTTTCAGACAGTCGCATAGCCGAACTGCTGGCGGAACAGGGCTTC
GTGGTCGCACGGCGCACCGTTGCGAAATACCGTGAGGCGCTCAAGATCCCGGCAGTCAACCTGCGCAAGTCTCTGTAG

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(141-332)

Sigma-54, DNA binding domain (Sigma54_DBD)

(348-503)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

67.976

100

0.685

Multiple sequence alignment