Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   BG588_RS05085 Genome accession   NZ_CP017541
Coordinates   1000067..1000546 (+) Length   159 a.a.
NCBI ID   WP_006248401.1    Uniprot ID   A0A249A0S4
Organism   Mannheimia haemolytica strain 1450     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 995067..1005546
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BG588_RS05055 (BG588_04990) - 995648..996412 (-) 765 WP_006248395.1 TSUP family transporter -
  BG588_RS05060 (BG588_04995) proB 996412..997496 (-) 1085 Protein_985 glutamate 5-kinase -
  BG588_RS05070 (BG588_05005) folA 997608..998102 (+) 495 WP_006248397.1 type 3 dihydrofolate reductase -
  BG588_RS05075 (BG588_05010) - 998222..998452 (+) 231 WP_006248398.1 YgjV family protein -
  BG588_RS05080 (BG588_05015) radA 998507..999886 (-) 1380 WP_006248399.1 DNA repair protein RadA -
  BG588_RS05085 (BG588_05020) pilA 1000067..1000546 (+) 480 WP_006248401.1 prepilin peptidase-dependent pilin Machinery gene
  BG588_RS05090 (BG588_05025) - 1000567..1001010 (+) 444 Protein_990 pilus assembly protein PilB -
  BG588_RS05095 (BG588_05030) pilC 1001016..1002212 (+) 1197 WP_006248403.1 type II secretion system F family protein Machinery gene
  BG588_RS05100 (BG588_05035) - 1002215..1002871 (+) 657 WP_006248404.1 prepilin peptidase -
  BG588_RS05105 (BG588_05040) coaE 1002907..1003542 (+) 636 WP_006248405.1 dephospho-CoA kinase -
  BG588_RS05110 (BG588_05045) yacG 1003524..1003709 (+) 186 WP_006248406.1 DNA gyrase inhibitor YacG -
  BG588_RS05115 (BG588_05050) holA 1003710..1004735 (-) 1026 WP_006253426.1 DNA polymerase III subunit delta -
  BG588_RS05120 (BG588_05055) lptE 1004778..1005266 (-) 489 WP_006248408.1 LPS assembly lipoprotein LptE -

Sequence

Protein


Download         Length: 159 a.a.        Molecular weight: 16841.15 Da        Isoelectric Point: 8.7964

>NTDB_id=200042 BG588_RS05085 WP_006248401.1 1000067..1000546(+) (pilA) [Mannheimia haemolytica strain 1450]
MQKIVHFRPLGIRQLHRAFTLIELMIVIAIIAILATIAIPSYNSYTQKAALSELLQASASYKSDVEVCIYNSGGLDNCSG
GTNGIQANKTSSNTTKYLNSIAVTKGVITVTGKGSLNGYGYTLTPSFSNNTISWTASCSGNDTSLFPANFCSTTVTPNG

Nucleotide


Download         Length: 480 bp        

>NTDB_id=200042 BG588_RS05085 WP_006248401.1 1000067..1000546(+) (pilA) [Mannheimia haemolytica strain 1450]
ATGCAAAAAATTGTACATTTTAGACCGCTTGGCATTCGCCAACTGCATCGGGCATTTACCCTCATTGAGTTGATGATCGT
GATTGCGATTATTGCCATTCTTGCCACTATTGCGATCCCTTCCTATAACTCATACACCCAAAAGGCGGCGTTATCGGAGT
TATTACAAGCATCTGCTTCGTATAAATCAGACGTTGAAGTCTGTATTTACAACAGTGGCGGGTTGGATAACTGCTCCGGT
GGCACGAATGGGATTCAGGCGAATAAAACCAGTAGTAATACCACTAAATACCTAAACTCAATTGCGGTAACAAAAGGCGT
GATTACGGTAACCGGCAAAGGCTCTCTGAATGGTTATGGCTATACCTTAACACCGTCGTTCTCAAATAATACGATTAGTT
GGACGGCAAGTTGTAGTGGCAATGATACGAGCCTATTTCCAGCTAATTTCTGTAGCACAACTGTTACACCAAACGGCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(48-148)

Prokaryotic N-terminal methylation motif (N_methyl)

(16-38)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A249A0S4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

66.038

100

0.66
  pilA Haemophilus influenzae 86-028NP

56.429

88.05

0.497
  pilA Haemophilus influenzae Rd KW20

54.93

89.308

0.491

Multiple sequence alignment