Detailed information NTG0199893

Bioinformatically predicted

Overview

• Name: rpoS
• Type: Regulator
• Locus tag: CFI09_RS05325
• Genome accession: NZ_CP022279
• Coordinates: 1048187..1049179 (+)
• Length: 330 a.a.
• NCBI ID: WP_000081550.1
• Uniprot ID: A0A370V765
• Organism: Escherichia coli strain STEC299
• Function: regulation of chitinases (predicted from homology) Competence regulation

Genomic Context

Location: 1043187..1054179

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CFI09_RS05295 (CFI09_05305)	ispD	1043248..1043958 (+)	711	WP_000246149.1	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase	-
CFI09_RS05300 (CFI09_05310)	ispF	1043958..1044437 (+)	480	WP_001219237.1	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase	-
CFI09_RS05305 (CFI09_05315)	truD	1044434..1045483 (+)	1050	WP_000568931.1	tRNA pseudouridine(13) synthase TruD	-
CFI09_RS05310 (CFI09_05320)	surE	1045464..1046225 (+)	762	WP_001295182.1	5'/3'-nucleotidase SurE	-
CFI09_RS05315 (CFI09_05325)	pcm	1046219..1046845 (+)	627	WP_000254701.1	protein-L-isoaspartate O-methyltransferase	-
CFI09_RS05320 (CFI09_05330)	nlpD	1047030..1048124 (+)	1095	WP_306174120.1	murein hydrolase activator NlpD	-
CFI09_RS05325 (CFI09_05335)	rpoS	1048187..1049179 (+)	993	WP_000081550.1	RNA polymerase sigma factor RpoS	Regulator
CFI09_RS05330 (CFI09_05340)	-	1049350..1049901 (-)	552	WP_001179442.1	flavodoxin family protein	-
CFI09_RS05335 (CFI09_05345)	-	1050126..1051169 (+)	1044	WP_001161584.1	MBL fold metallo-hydrolase	-
CFI09_RS05340 (CFI09_05350)	ygbN	1051312..1052676 (-)	1365	WP_000104433.1	GntP family transporter	-
CFI09_RS05345 (CFI09_05355)	ygbM	1052765..1053541 (-)	777	WP_001136912.1	HPr family phosphocarrier protein	-

Sequence

Protein

• Length: 330 a.a.
• Molecular weight: 37972.84 Da
• Isoelectric Point: 4.5597

>NTDB_id=199893 CFI09_RS05325 WP_000081550.1 1048187..1049179(+) (rpoS) [Escherichia coli strain STEC299]
MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFA
RRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILAD
EKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQ
GLNIEALFRE

Nucleotide

• Length: 993 bp

>NTDB_id=199893 CFI09_RS05325 WP_000081550.1 1048187..1049179(+) (rpoS) [Escherichia coli strain STEC299]
ATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGA
AAAGGCCTTAGTAGAAGAGGAACCCAGTGATAACGATTTGGCCGAAGAGGAACTGTTATCGCAGGGAGCCACACAGCGTG
TGTTGGACGCGACTCAGCTTTACCTTGGTGAGATTGGTTATTCACCACTGTTAACGGCCGAAGAAGAAGTTTATTTTGCG
CGTCGCGCACTGCGTGGAGATGTCGCCTCTCGCCGCCGGATGATCGAGAGTAACTTGCGTCTGGTGGTAAAAATTGCCCG
CCGTTATGGCAATCGTGGTCTGGCGTTGCTGGACCTGATCGAAGAGGGCAACCTGGGGCTGATCCGCGCGGTAGAGAAGT
TTGACCCGGAACGTGGTTTCCGCTTCTCAACATACGCAACCTGGTGGATTCGCCAGACGATCGAACGGGCGATTATGAAC
CAAACCCGTACTATTCGTTTGCCGATTCACATCGTAAAGGAGCTGAACGTTTACCTGCGAACCGCACGTGAGTTGTCCCA
TAAGCTGGACCACGAACCAAGTGCGGAAGAGATCGCAGAGCAACTGGATAAGCCAGTTGATGACGTCAGCCGTATGCTTC
GTCTTAACGAGCGCATTACCTCGGTAGACACCCCGCTGGGTGGTGATTCCGAAAAAGCGTTGCTGGACATCCTGGCCGAT
GAAAAAGAGAATGGTCCGGAAGATACCACGCAAGATGACGATATGAAGCAGAGCATCGTCAAATGGCTGTTCGAGCTGAA
CGCCAAACAGCGTGAAGTACTGGCACGTCGATTCGGTTTGCTGGGGTACGAAGCGGCAACACTGGAAGATGTAGGTCGTG
AAATTGGCCTCACCCGTGAACGTGTTCGCCAGATTCAGGTTGAAGGCCTGCGCCGTTTGCGCGAAATCCTGCAAACGCAG
GGGCTGAATATCGAAGCGCTGTTCCGCGAGTAA

Domains

Predicted by InterProScan.

• Sigma-70 region 3 (Sigma70_r3) (174-248)

• Sigma-70 region 2 (Sigma70_r2) (94-163)

• Sigma-70 factor, region 1.2 (Sigma70_r1_2) (56-88)

• Sigma-70, region 4 (Sigma70_r4) (262-315)

3D structure

• AlphaFold DB: A0A370V765

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rpoS	Vibrio cholerae O1 biovar El Tor strain E7946	73.065	97.879	0.715