Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   CFI09_RS00460 Genome accession   NZ_CP022279
Coordinates   87108..87776 (+) Length   222 a.a.
NCBI ID   WP_001296533.1    Uniprot ID   B7N279
Organism   Escherichia coli strain STEC299     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 82108..92776
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CFI09_RS00425 (CFI09_00425) yicC 82128..82991 (-) 864 WP_000621331.1 YicC/YloC family endoribonuclease -
  CFI09_RS00435 (CFI09_00435) rph 83118..83834 (+) 717 WP_001247093.1 ribonuclease PH -
  CFI09_RS00440 (CFI09_00440) pyrE 83899..84540 (+) 642 WP_000806177.1 orotate phosphoribosyltransferase -
  CFI09_RS00445 (CFI09_00445) slmA 84577..85173 (-) 597 WP_000818603.1 nucleoid occlusion factor SlmA -
  CFI09_RS00450 (CFI09_00450) dut 85280..85738 (-) 459 WP_000976066.1 dUTP diphosphatase -
  CFI09_RS00455 (CFI09_00455) coaBC 85716..86936 (-) 1221 WP_000050149.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  CFI09_RS00460 (CFI09_00460) radC 87108..87776 (+) 669 WP_001296533.1 DNA repair protein RadC Machinery gene
  CFI09_RS00465 (CFI09_00465) rpmB 87993..88229 (+) 237 WP_000091955.1 50S ribosomal protein L28 -
  CFI09_RS00470 (CFI09_00470) rpmG 88250..88417 (+) 168 WP_001051798.1 50S ribosomal protein L33 -
  CFI09_RS00475 (CFI09_00475) mutM 88515..89324 (+) 810 WP_001114533.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  CFI09_RS00480 (CFI09_00480) coaD 89363..89842 (-) 480 WP_001171873.1 pantetheine-phosphate adenylyltransferase -
  CFI09_RS00485 (CFI09_00485) waaA 89850..91127 (-) 1278 WP_101973409.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  CFI09_RS00490 (CFI09_00490) rfaQ 91540..92598 (+) 1059 WP_000360417.1 lipopolysaccharide core heptosyltransferase RfaQ -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25286.40 Da        Isoelectric Point: 7.9748

>NTDB_id=199874 CFI09_RS00460 WP_001296533.1 87108..87776(+) (radC) [Escherichia coli strain STEC299]
MKNNVQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=199874 CFI09_RS00460 WP_001296533.1 87108..87776(+) (radC) [Escherichia coli strain STEC299]
GTGAAAAACAATGTACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACGGATGTCGAGCT
GCTGGCGCTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAGCAATTTAGTGGCGTTCATGGAATTGGCGTGGCGAAATTCGCCCAGTTA
AAGGGGATTGCCGAACTGGCGCGGCGTTACTACAACGTACGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GAGGGAATTTTTACAAAGCCAGCTCACGGGCGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCCCAACACAGGG
TTATAACGCATAGCCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCGCATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

UPF0758 N-terminal (UPF0758_N)

(10-77)

RadC-like JAB domain (RadC)

(103-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7N279

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496

Multiple sequence alignment