Detailed information NTG0199725

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: CFA72_RS02855
• Genome accession: NZ_CP022206
• Coordinates: 529331..531715 (+)
• Length: 794 a.a.
• NCBI ID: WP_093974591.1
• Uniprot ID: -
• Organism: Streptococcus pyogenes strain GURSA1
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 524331..536715

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CFA72_RS02835 (CFA72_02835)	-	525555..526451 (+)	897	WP_010922543.1	LysR family transcriptional regulator	-
CFA72_RS02840 (CFA72_02840)	-	526581..528188 (+)	1608	WP_002988954.1	ribonuclease Y	-
CFA72_RS02845 (CFA72_02845)	gmk	528298..528933 (+)	636	WP_002983649.1	guanylate kinase	-
CFA72_RS02850 (CFA72_02850)	rpoZ	528949..529266 (+)	318	WP_002983650.1	DNA-directed RNA polymerase subunit omega	-
CFA72_RS02855 (CFA72_02855)	priA	529331..531715 (+)	2385	WP_093974591.1	primosomal protein N'	Machinery gene
CFA72_RS02860 (CFA72_02860)	fmt	531777..532712 (+)	936	WP_012560856.1	methionyl-tRNA formyltransferase	-
CFA72_RS02865 (CFA72_02865)	rsmB	532702..534024 (+)	1323	WP_023609899.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
CFA72_RS02870 (CFA72_02870)	-	534062..534802 (+)	741	WP_002983660.1	Stp1/IreP family PP2C-type Ser/Thr phosphatase	-
CFA72_RS02875 (CFA72_02875)	pknB	534799..536697 (+)	1899	WP_002994648.1	Stk1 family PASTA domain-containing Ser/Thr kinase	Regulator

Sequence

Protein

• Length: 794 a.a.
• Molecular weight: 90380.31 Da
• Isoelectric Point: 9.4590

>NTDB_id=199725 CFA72_RS02855 WP_093974591.1 529331..531715(+) (priA) [Streptococcus pyogenes strain GURSA1]
MEKVAHVIVDIPLMQTDKPFSYGIPKELVSLVQLGSRVHVPFGKGNRLLQGFIIGFGQEDSSSLKLIQTVLDPEPVLNQE
QLTLADQLRKTVFSYKITLLKAMIPNLLNSNYDKVLRPESGLKKSDRDFLFEGKPSVLYSTLDREKEKIALKGIQTGHIT
VSYLAKDKKNLKTEKYYHVDLDALAVHPISSRAKKRQLLKDYLLTHTKEAKLATLYQAFSRDVVAYFVTNHLIRIDERPI
DRSESYFDQIKPSSFLTLNEQQASAVTEIVEQIGKPSKPFLIEGITGSGKTEVYLHIIEAVLKQDKTAIVLVPEISLTPQ
MTSRFISRFGKQVAIMHSGLSDGEKFDEWRKIKTGQAKVVVGARSAIFSPLERIGAIIIDEEHESTYKQESNPRYHAREV
ALLRAKHHQAVVVMGSATPSIESRARASKGVYHFIQLTQRANPLAKIPEVTIVDFRDYIGQQAVSNFTPYLIDKIKERLV
KKEQVVLMLNRRGYSSFVMCRDCGYVDKCPNCDISLTLHMDTKTMNCHYCGFQKTIPITCPECHSNSIRYYGTGTQKAFD
ELQGVIPEARILRMDVDTTRKKRSHKTILDSFGRQEADILLGTQMIAKGLDFPNVTLVGVLNADTSLNLPDFRASEKTFQ
LLTQVAGRAGRAHKPGEVLIQTYNPDHYAIQLAKKQDFEAFYRYEMAIRRQMAYPPYYFTVGITLSHRLEASVVKKAYQV
TELLKSHLSDNIKILGPTPKPIARTHNLYHYQILLKYRFEDNLEETLNRILDWSQEADNRHLKLIIDCEPQQFL

Nucleotide

• Length: 2385 bp

>NTDB_id=199725 CFA72_RS02855 WP_093974591.1 529331..531715(+) (priA) [Streptococcus pyogenes strain GURSA1]
ATGGAAAAAGTAGCTCATGTGATTGTAGATATTCCATTGATGCAAACGGACAAGCCATTTTCTTATGGTATTCCAAAAGA
GTTAGTTTCTCTCGTGCAGCTTGGTTCTCGTGTGCATGTTCCTTTTGGAAAAGGGAATCGTTTGTTGCAAGGCTTTATTA
TTGGTTTTGGCCAAGAAGATTCAAGCTCTCTTAAACTGATTCAGACCGTTTTAGATCCTGAGCCAGTCCTAAACCAAGAA
CAATTGACCCTTGCTGATCAGCTTCGAAAAACAGTTTTTTCTTATAAAATCACGCTCTTAAAAGCAATGATTCCTAATCT
TCTTAACTCTAACTATGATAAAGTGCTAAGGCCAGAGTCAGGTCTAAAAAAGTCTGATAGGGACTTTCTTTTTGAAGGAA
AACCATCTGTCTTATATTCTACCTTAGACCGCGAGAAGGAAAAAATAGCTCTTAAGGGCATACAAACTGGGCATATTACT
GTTAGTTATTTAGCCAAAGACAAGAAAAACCTAAAGACAGAAAAATATTATCACGTCGACTTAGACGCCTTAGCGGTACA
TCCCATCTCTAGTCGTGCCAAAAAGCGTCAATTATTAAAAGACTATCTGCTAACCCATACGAAGGAAGCTAAACTTGCAA
CGCTTTATCAAGCCTTTTCTAGAGATGTGGTGGCTTACTTTGTGACAAATCACTTAATTCGTATTGACGAACGTCCAATT
GATCGCAGTGAGTCCTATTTTGACCAGATCAAACCATCTTCTTTTTTAACCTTAAATGAACAGCAGGCGTCAGCTGTTAC
TGAAATTGTTGAACAAATTGGGAAACCGAGTAAACCTTTTTTGATTGAAGGGATTACAGGTTCGGGTAAGACAGAAGTTT
ATCTTCATATCATCGAAGCGGTTTTAAAACAGGATAAGACAGCCATTGTTTTAGTGCCAGAAATTTCGTTGACTCCTCAG
ATGACCTCTCGTTTTATTTCCCGTTTCGGAAAGCAAGTGGCTATTATGCATTCAGGCTTATCAGATGGTGAAAAGTTTGA
TGAGTGGCGCAAGATAAAAACAGGACAGGCTAAGGTTGTGGTTGGTGCCCGCTCTGCTATTTTCTCCCCACTAGAGCGGA
TTGGTGCAATTATTATCGATGAGGAGCATGAATCAACCTATAAACAAGAAAGCAACCCTAGATACCATGCCAGAGAGGTT
GCCCTGCTCAGAGCTAAACACCATCAAGCAGTTGTGGTTATGGGCTCTGCTACGCCAAGTATTGAAAGCCGAGCAAGGGC
TAGTAAGGGTGTCTATCATTTTATTCAATTAACGCAAAGAGCAAACCCTCTTGCTAAGATTCCAGAAGTAACGATTGTTG
ATTTTCGTGATTATATTGGACAGCAAGCAGTTAGTAATTTTACCCCATATTTGATTGATAAGATTAAAGAACGTTTGGTT
AAAAAAGAACAAGTTGTCTTAATGTTAAATAGACGTGGCTATTCTAGTTTTGTGATGTGTCGGGACTGTGGTTATGTGGA
CAAATGTCCCAACTGTGATATCTCTTTAACCCTTCACATGGATACTAAAACTATGAATTGCCATTACTGTGGGTTTCAAA
AAACTATCCCAATTACTTGTCCTGAGTGTCATAGCAATAGTATCCGCTATTATGGCACTGGAACTCAAAAAGCTTTTGAT
GAATTGCAAGGGGTTATCCCAGAAGCTAGGATCTTGAGAATGGATGTTGACACTACACGAAAAAAAAGAAGTCACAAAAC
CATACTAGATAGTTTTGGTCGCCAGGAAGCAGATATTTTGTTAGGGACGCAAATGATTGCCAAAGGTCTGGATTTTCCAA
ATGTGACTTTAGTTGGGGTACTAAATGCAGATACCTCCTTAAATTTGCCAGATTTTCGAGCGTCAGAAAAAACGTTTCAA
TTGTTAACACAGGTTGCTGGTCGGGCAGGCCGTGCCCATAAGCCAGGAGAAGTCTTGATTCAAACTTATAATCCCGATCA
CTATGCTATTCAATTAGCTAAGAAACAAGATTTTGAAGCCTTTTACCGTTATGAAATGGCGATTCGCCGTCAGATGGCTT
ATCCCCCTTACTACTTTACAGTGGGTATTACGCTTTCGCATCGTTTAGAAGCATCGGTTGTAAAAAAAGCTTATCAAGTT
ACAGAACTGCTAAAGAGTCACTTATCGGATAATATTAAAATATTAGGACCGACTCCTAAGCCAATTGCTAGAACACACAA
TTTATACCACTATCAAATTTTGTTAAAATACCGTTTTGAAGATAACCTAGAAGAGACGCTCAATCGTATTCTTGATTGGA
GTCAAGAAGCAGATAATCGTCATTTAAAACTAATTATTGATTGTGAACCGCAACAATTTTTATAA

Domains

Predicted by InterProScan.

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (509-534)

• 3DNA-binding domain (3BD) (PriA_3primeBD) (7-105)

• Primosomal protein N C-terminal domain (PriA_C) (696-790)

• Type III restriction enzyme, res subunit (ResIII) (257-420)

• Helicase conserved C-terminal domain (Helicase_C) (568-652)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Bacillus subtilis subsp. subtilis str. 168	46.708	100	0.474