Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   CE141_RS00630 Genome accession   NZ_CP022164
Coordinates   125384..126052 (+) Length   222 a.a.
NCBI ID   WP_001297375.1    Uniprot ID   P65957
Organism   Escherichia coli strain M160133     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 120384..131052
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CE141_RS00605 (CE141_00605) pyrE 120909..121550 (+) 642 WP_000806177.1 orotate phosphoribosyltransferase -
  CE141_RS00610 (CE141_00610) - 121848..122807 (+) 960 WP_022646277.1 reverse transcriptase family protein -
  CE141_RS00615 (CE141_00615) slmA 122853..123449 (-) 597 WP_000818603.1 nucleoid occlusion factor SlmA -
  CE141_RS00620 (CE141_00620) dut 123556..124014 (-) 459 WP_000976070.1 dUTP diphosphatase -
  CE141_RS00625 (CE141_00625) coaBC 123992..125212 (-) 1221 WP_000050149.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  CE141_RS00630 (CE141_00630) radC 125384..126052 (+) 669 WP_001297375.1 DNA repair protein RadC Machinery gene
  CE141_RS00635 (CE141_00635) rpmB 126269..126505 (+) 237 WP_000091955.1 50S ribosomal protein L28 -
  CE141_RS00640 (CE141_00640) rpmG 126526..126693 (+) 168 WP_001051798.1 50S ribosomal protein L33 -
  CE141_RS00645 (CE141_00645) mutM 126791..127600 (+) 810 WP_001114533.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  CE141_RS00650 (CE141_00650) coaD 127639..128118 (-) 480 WP_001171866.1 pantetheine-phosphate adenylyltransferase -
  CE141_RS00655 (CE141_00655) waaA 128126..129403 (-) 1278 WP_000891562.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  CE141_RS00660 (CE141_00660) rfaQ 129816..130874 (+) 1059 WP_000360409.1 lipopolysaccharide core heptosyltransferase RfaQ -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25258.35 Da        Isoelectric Point: 7.9748

>NTDB_id=199610 CE141_RS00630 WP_001297375.1 125384..126052(+) (radC) [Escherichia coli strain M160133]
MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=199610 CE141_RS00630 WP_001297375.1 125384..126052(+) (radC) [Escherichia coli strain M160133]
GTGAAAAACAATGCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACGGATGTCGAGCT
GCTGGCGCTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAGCAATTTAGTGGCGTTCATGGAATTGGCGTGGCGAAATTTGCCCAGTTA
AAGGGGATTGCTGAACTGGCGCGGCGTTACTACAACGTACGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GCGGGAATTTTTACAAAGCCAGCTCACGGGCGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCCCAGCACAGGG
TTATAACGCATAGCCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCGCATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

RadC-like JAB domain (RadC)

(103-220)

UPF0758 N-terminal (UPF0758_N)

(9-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P65957

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496