Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   AM434_RS10820 Genome accession   NZ_CP021935
Coordinates   2125098..2125766 (-) Length   222 a.a.
NCBI ID   WP_001296533.1    Uniprot ID   B7N279
Organism   Escherichia coli strain AR_0055     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2120098..2130766
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AM434_RS10790 (AM434_10800) rfaQ 2120275..2121333 (-) 1059 WP_000360408.1 lipopolysaccharide core heptosyltransferase RfaQ -
  AM434_RS10795 (AM434_10805) waaA 2121747..2123024 (+) 1278 WP_000891564.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  AM434_RS10800 (AM434_10810) coaD 2123032..2123511 (+) 480 WP_001171854.1 pantetheine-phosphate adenylyltransferase -
  AM434_RS10805 (AM434_10815) mutM 2123550..2124359 (-) 810 WP_001114533.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  AM434_RS10810 (AM434_10820) rpmG 2124457..2124624 (-) 168 WP_001051798.1 50S ribosomal protein L33 -
  AM434_RS10815 (AM434_10825) rpmB 2124645..2124881 (-) 237 WP_000091955.1 50S ribosomal protein L28 -
  AM434_RS10820 (AM434_10830) radC 2125098..2125766 (-) 669 WP_001296533.1 DNA repair protein RadC Machinery gene
  AM434_RS10825 (AM434_10835) coaBC 2125938..2127158 (+) 1221 WP_000050112.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  AM434_RS10830 (AM434_10840) dut 2127136..2127594 (+) 459 WP_000976070.1 dUTP diphosphatase -
  AM434_RS10835 (AM434_10845) slmA 2127701..2128297 (+) 597 WP_000818603.1 nucleoid occlusion factor SlmA -
  AM434_RS10840 (AM434_10850) pyrE 2128334..2128975 (-) 642 WP_000806177.1 orotate phosphoribosyltransferase -
  AM434_RS10845 (AM434_10855) rph 2129041..2129757 (-) 717 WP_001247093.1 ribonuclease PH -
  AM434_RS10855 (AM434_10865) yicC 2129884..2130747 (+) 864 WP_000621332.1 YicC/YloC family endoribonuclease -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25286.40 Da        Isoelectric Point: 7.9748

>NTDB_id=198577 AM434_RS10820 WP_001296533.1 2125098..2125766(-) (radC) [Escherichia coli strain AR_0055]
MKNNVQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=198577 AM434_RS10820 WP_001296533.1 2125098..2125766(-) (radC) [Escherichia coli strain AR_0055]
GTGAAAAACAATGTACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACGGATGTCGAGCT
GCTGGCGCTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAGCAATTTAGTGGCGTTCATGGAATTGGCGTGGCGAAATTCGCCCAGTTA
AAGGGGATTGCCGAACTGGCGCGGCGTTACTACAACGTACGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GAGGGAATTTTTACAAAGCCAGCTCACGGGCGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCCCAACACAGGG
TTATAACGCATAGCCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCGCATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

UPF0758 N-terminal (UPF0758_N)

(10-77)

RadC-like JAB domain (RadC)

(103-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7N279

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496

Multiple sequence alignment