Detailed information    

insolico Bioinformatically predicted

Overview

Name   waaF   Type   Regulator
Locus tag   CJJRM3420_RS05585 Genome accession   NZ_CP017456
Coordinates   1084074..1085033 (+) Length   319 a.a.
NCBI ID   WP_002856368.1    Uniprot ID   -
Organism   Campylobacter jejuni subsp. jejuni str. RM3420     
Function   repress natural transformation (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1066391..1089085 1084074..1085033 within 0


Gene organization within MGE regions


Location: 1066391..1089085
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CJJRM3420_RS05495 (CjjRM3420_1084) pglI 1066391..1067320 (-) 930 WP_002856262.1 GalNAc(5)-diNAcBac-PP-undecaprenol beta-1,3-glucosyltransferase -
  CJJRM3420_RS05500 (CjjRM3420_1085) pglH 1067313..1068392 (-) 1080 WP_002911763.1 GalNAc-alpha-(1->4)-GalNAc-alpha-(1->3)- diNAcBac-PP-undecaprenol alpha-1,4-N-acetyl-D-galactosaminyltransferase -
  CJJRM3420_RS05505 (CjjRM3420_1086) pglK 1068389..1070083 (-) 1695 WP_002856016.1 ABC-type lipopolysaccharide transporter PglK -
  CJJRM3420_RS05510 (CjjRM3420_1087) galE 1070077..1071063 (-) 987 WP_002856461.1 UDP-glucose 4-epimerase GalE -
  CJJRM3420_RS05515 (CjjRM3420_1088) - 1071121..1071915 (-) 795 WP_002856546.1 3'-5' exonuclease -
  CJJRM3420_RS05520 (CjjRM3420_1089) waaC 1071981..1073009 (+) 1029 WP_002855693.1 lipopolysaccharide heptosyltransferase I -
  CJJRM3420_RS05525 (CjjRM3420_1090) htrB 1072999..1073886 (+) 888 WP_002855840.1 lipid A biosynthesis lauroyl acyltransferase HtrB -
  CJJRM3420_RS05530 (CjjRM3420_1091) - 1073883..1075431 (+) 1549 Protein_1070 glycosyltransferase family 2 protein -
  CJJRM3420_RS05540 (CjjRM3420_1092) - 1075435..1076604 (+) 1170 WP_002852863.1 glycosyltransferase family 2 protein -
  CJJRM3420_RS05545 (CjjRM3420_1093) - 1076674..1077717 (+) 1044 WP_002912176.1 beta-1,4-N-acetylgalactosaminyltransferase -
  CJJRM3420_RS05550 (CjjRM3420_1094) - 1077703..1078608 (-) 906 WP_002852740.1 glycosyltransferase family 2 protein -
  CJJRM3420_RS05555 (CjjRM3420_1095) - 1078697..1079572 (+) 876 WP_002869021.1 alpha-2,3-sialyltransferase -
  CJJRM3420_RS05560 (CjjRM3420_1096) neuB 1079573..1080613 (+) 1041 WP_002870496.1 N-acetylneuraminate synthase -
  CJJRM3420_RS05565 (CjjRM3420_1097) neuC 1080610..1081728 (+) 1119 WP_002911676.1 UDP-N-acetylglucosamine 2-epimerase -
  CJJRM3420_RS05570 (CjjRM3420_1098) - 1081725..1082390 (+) 666 WP_002855897.1 cytidylyltransferase domain-containing protein -
  CJJRM3420_RS05575 (CjjRM3420_1099) - 1082378..1083211 (+) 834 WP_002855772.1 CatB-related O-acetyltransferase -
  CJJRM3420_RS05580 (CjjRM3420_1100) waaV 1083203..1084015 (-) 813 WP_002856066.1 glycosyltransferase family 2 protein -
  CJJRM3420_RS05585 (CjjRM3420_1101) waaF 1084074..1085033 (+) 960 WP_002856368.1 lipopolysaccharide heptosyltransferase II Regulator
  CJJRM3420_RS05590 (CjjRM3420_1102) gmhA 1085014..1085574 (-) 561 WP_002855785.1 D-sedoheptulose 7-phosphate isomerase -
  CJJRM3420_RS05595 (CjjRM3420_1103) rfaE1 1085571..1086965 (-) 1395 WP_032581686.1 D-glycero-beta-D-manno-heptose-7-phosphate kinase -
  CJJRM3420_RS05600 (CjjRM3420_1104) rfaD 1086958..1087911 (-) 954 WP_002856483.1 ADP-glyceromanno-heptose 6-epimerase -
  CJJRM3420_RS05605 (CjjRM3420_1105) gmhB 1087912..1088472 (-) 561 WP_002911679.1 D-glycero-alpha-D-manno-heptose-1,7-bisphosphate 7-phosphatase -
  CJJRM3420_RS05610 (CjjRM3420_1106) - 1088554..1088856 (+) 303 WP_002852762.1 c-type cytochrome -
  CJJRM3420_RS05615 (CjjRM3420_1107) ccoS 1088879..1089085 (-) 207 WP_002852798.1 cbb3-type cytochrome oxidase assembly protein CcoS -

Sequence

Protein


Download         Length: 319 a.a.        Molecular weight: 36719.17 Da        Isoelectric Point: 10.1941

>NTDB_id=198335 CJJRM3420_RS05585 WP_002856368.1 1084074..1085033(+) (waaF) [Campylobacter jejuni subsp. jejuni str. RM3420]
MKIFIHLPTWLGDAVMASPALYAIKEHFKDAQFILYGSLVSTALFKEFPNSKIIIENKQSRYKQALSLRKELGKIDFSFA
FRSAFSSKIILHILKTKQRYFFDKNKHKEEHQVLKYLYFIENSLDIKAHFKDLKLPFKLKFQNPLILKNGKKILGLNPGA
SFGSAKRWDASYFAKVALNFSQSHEILIFGAGKAEQELCNEIYQILKEQNIKVKNLCNKTTIKTLCQNIAFCDLFITNDS
GPMHLSAVYKVKTVAIFGPTNFTQTSPWQNENAKLVHLDLACMPCMQKTCPLKHHKCMKDLKPQRVIEEARNLLKNSHL

Nucleotide


Download         Length: 960 bp        

>NTDB_id=198335 CJJRM3420_RS05585 WP_002856368.1 1084074..1085033(+) (waaF) [Campylobacter jejuni subsp. jejuni str. RM3420]
ATGAAAATTTTTATACACCTTCCAACTTGGCTAGGCGATGCGGTGATGGCTTCACCTGCTTTATACGCTATAAAAGAACA
CTTTAAAGATGCCCAGTTTATCCTTTATGGCTCTTTGGTTTCCACAGCACTTTTTAAAGAATTTCCTAATTCTAAAATCA
TCATAGAAAATAAACAATCCCGTTATAAACAAGCCCTATCTTTACGCAAAGAACTTGGTAAGATCGATTTTAGCTTTGCT
TTTAGATCTGCGTTTTCTTCTAAGATTATCTTACATATTCTCAAAACAAAACAAAGATATTTTTTTGACAAAAACAAGCA
CAAAGAAGAACATCAAGTTTTAAAATACCTTTATTTTATAGAAAACTCACTTGACATAAAAGCTCATTTTAAAGACTTAA
AACTTCCCTTTAAGCTAAAATTTCAAAACCCTCTTATCTTAAAAAATGGCAAAAAAATTCTAGGACTCAACCCTGGCGCA
AGCTTTGGAAGTGCAAAAAGATGGGATGCGAGTTATTTTGCTAAAGTGGCTTTAAATTTCAGCCAAAGTCATGAAATTTT
AATCTTTGGCGCAGGAAAAGCCGAACAAGAACTTTGTAATGAAATTTATCAAATTTTAAAAGAACAAAACATAAAAGTAA
AAAATCTTTGCAATAAAACCACCATCAAAACCCTTTGTCAAAATATCGCTTTTTGCGATCTTTTCATCACAAATGACAGT
GGTCCTATGCACCTAAGTGCGGTTTATAAGGTAAAAACCGTAGCTATTTTTGGCCCCACAAATTTTACCCAAACTTCACC
TTGGCAAAATGAAAACGCAAAATTAGTACATTTAGATCTAGCTTGCATGCCTTGTATGCAAAAAACCTGTCCTTTAAAAC
ACCACAAATGCATGAAAGATCTAAAGCCTCAAAGGGTTATAGAAGAGGCTAGAAATTTACTTAAAAACTCTCATCTATGA

Domains

Predicted by InterproScan.

Glycosyltransferase family 9 (heptosyltransferase) (Glyco_transf_9)

(95-294)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  waaF Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

95.611

100

0.956

Multiple sequence alignment