Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   BI283_RS11830 Genome accession   NZ_CP017430
Coordinates   2463118..2464488 (-) Length   456 a.a.
NCBI ID   WP_002481315.1    Uniprot ID   -
Organism   Staphylococcus simulans strain MR4     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2458118..2469488
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BI283_RS11815 (BI283_11715) cysE 2459356..2459985 (-) 630 WP_172954255.1 serine O-acetyltransferase -
  BI283_RS11820 (BI283_11720) gltX 2460285..2461739 (-) 1455 WP_002481317.1 glutamate--tRNA ligase -
  BI283_RS11825 (BI283_11725) - 2462026..2463090 (-) 1065 WP_106421106.1 PIN/TRAM domain-containing protein -
  BI283_RS11830 (BI283_11730) radA 2463118..2464488 (-) 1371 WP_002481315.1 DNA repair protein RadA Machinery gene
  BI283_RS11835 (BI283_11735) - 2464776..2467241 (-) 2466 WP_057510126.1 ATP-dependent Clp protease ATP-binding subunit -
  BI283_RS11840 (BI283_11740) - 2467253..2468257 (-) 1005 WP_106421107.1 protein arginine kinase -
  BI283_RS11845 (BI283_11745) - 2468261..2468683 (-) 423 Protein_2318 UvrB/UvrC motif-containing protein -
  BI283_RS11850 (BI283_11750) - 2468785..2469246 (-) 462 WP_023016300.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 50252.78 Da        Isoelectric Point: 7.4601

>NTDB_id=198077 BI283_RS11830 WP_002481315.1 2463118..2464488(-) (radA) [Staphylococcus simulans strain MR4]
MAKKKVTFECMACGYQSPKWMGKCPNCGAWNQMEEVMTQKEAPSGRGMRTREQTAKVTKLNDVQHESTPRIHTSSREFDR
VLGGGIVQGSLVLIGGDPGIGKSTLLLQICSALSQNKKVLYITGEESLNQTKLRADRLEEDSSQLNVFAETDLEVIKEAV
KQTQPDLVVVDSIQTVYHPEISSAPGSVSQVRESTQILMGIAKQMNIATFIVGHVTKEGQIAGPRLLEHMVDTVLYFEGD
EHHAYRILRAVKNRFGSTNEMGIFEMKHSGLKGVKNPSEMFLEERSTNVAGSTIVPTMEGTRPLLIEVQALVTPTTFNNP
RRMATGIDHNRLNLLMAVLEKKESYLLQQQDAYVKVAGGVRLTEPAVDLAIIAAVASSFKDQPINGMDCFVGEVGLTGEV
RRVSRIEQRVQEAAKLGFKRVIIPKTNIGGWDFPDNIQVIGVTSVHEALNFALMKS

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=198077 BI283_RS11830 WP_002481315.1 2463118..2464488(-) (radA) [Staphylococcus simulans strain MR4]
TTGGCAAAAAAGAAAGTGACATTTGAATGTATGGCATGTGGTTATCAATCACCGAAATGGATGGGGAAATGTCCGAATTG
TGGTGCTTGGAACCAAATGGAAGAAGTTATGACGCAAAAAGAAGCCCCAAGTGGCAGAGGGATGCGTACAAGAGAACAAA
CAGCTAAAGTAACAAAGTTAAATGATGTCCAACATGAATCTACACCGCGTATCCATACAAGTTCGAGAGAATTTGATCGT
GTCTTAGGTGGCGGTATTGTCCAAGGTTCGCTTGTGTTAATCGGTGGGGACCCTGGTATTGGTAAATCGACATTATTACT
TCAAATTTGTTCTGCGTTATCTCAAAATAAAAAAGTACTTTATATTACAGGTGAGGAATCACTGAATCAAACGAAATTGC
GAGCAGATCGTTTAGAAGAAGATTCAAGTCAATTAAACGTGTTTGCGGAAACAGATTTAGAAGTTATTAAAGAAGCTGTT
AAACAAACACAACCAGACTTAGTGGTGGTAGACTCGATTCAAACAGTGTATCATCCGGAAATCAGTTCAGCGCCTGGTTC
TGTCTCACAAGTACGAGAAAGTACGCAAATTCTGATGGGGATTGCTAAACAAATGAATATCGCAACCTTTATCGTCGGTC
ATGTGACGAAAGAAGGACAAATTGCAGGTCCACGTTTATTAGAACATATGGTAGATACGGTACTATATTTCGAAGGGGAC
GAACATCATGCATATAGAATTCTGCGTGCAGTGAAGAACCGTTTCGGTTCAACGAATGAAATGGGAATCTTCGAAATGAA
ACATAGCGGTTTGAAAGGAGTTAAGAACCCTTCAGAGATGTTCTTAGAAGAACGTTCAACGAATGTAGCTGGCTCTACAA
TTGTTCCGACAATGGAAGGTACACGTCCTTTATTAATTGAAGTGCAAGCATTAGTGACACCGACAACATTTAATAACCCA
AGACGTATGGCGACTGGTATCGATCATAACCGTTTAAATTTATTAATGGCAGTGTTAGAGAAGAAAGAAAGTTACTTGCT
GCAACAACAAGATGCTTATGTCAAAGTTGCAGGCGGCGTGCGTTTAACAGAACCAGCTGTTGATTTAGCAATTATCGCAG
CGGTTGCTTCTAGTTTTAAAGACCAACCTATCAACGGCATGGATTGTTTCGTCGGTGAAGTAGGTCTGACAGGAGAAGTA
CGACGTGTCTCACGTATTGAACAACGTGTTCAAGAAGCAGCGAAATTAGGTTTCAAACGTGTGATTATTCCTAAAACCAA
TATAGGAGGTTGGGACTTCCCAGATAATATTCAAGTTATCGGTGTAACCAGTGTGCATGAAGCACTGAACTTTGCATTGA
TGAAAAGCTGA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(360-427)

AAA domain (AAA_25)

(69-219)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

65.864

100

0.66
  radA Streptococcus pneumoniae Rx1

60.802

98.465

0.599
  radA Streptococcus pneumoniae D39

60.802

98.465

0.599
  radA Streptococcus pneumoniae R6

60.802

98.465

0.599
  radA Streptococcus pneumoniae TIGR4

60.802

98.465

0.599
  radA Streptococcus mitis NCTC 12261

60.802

98.465

0.599
  radA Streptococcus mitis SK321

60.802

98.465

0.599

Multiple sequence alignment