Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   AM384_RS16935 Genome accession   NZ_CP021879
Coordinates   3320720..3321730 (+) Length   336 a.a.
NCBI ID   WP_000568519.1    Uniprot ID   Q32HA1
Organism   Escherichia coli strain AR_0137     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3315720..3326730
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AM384_RS16895 (AM384_16830) nudB 3316668..3317120 (+) 453 WP_001300367.1 dihydroneopterin triphosphate diphosphatase -
  AM384_RS16900 (AM384_16835) yebC 3317149..3317889 (+) 741 WP_000907248.1 YebC/PmpR family DNA-binding transcriptional regulator -
  AM384_RS16905 (AM384_16840) ruvC 3317924..3318445 (+) 522 WP_001295503.1 crossover junction endodeoxyribonuclease RuvC -
  AM384_RS16910 (AM384_16845) yebB 3318447..3319049 (-) 603 WP_001024916.1 YebB family permuted papain-like enzyme -
  AM384_RS16915 (AM384_16850) yobI 3319119..3319184 (+) 66 WP_001386853.1 stress response small protein YobI -
  AM384_RS16930 (AM384_16865) ruvA 3320100..3320711 (+) 612 WP_000580323.1 Holliday junction branch migration protein RuvA -
  AM384_RS16935 (AM384_16870) ruvB 3320720..3321730 (+) 1011 WP_000568519.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  AM384_RS16940 (AM384_16875) znuB 3321974..3322759 (-) 786 WP_000571478.1 zinc ABC transporter permease subunit ZnuB -
  AM384_RS16945 (AM384_16880) znuC 3322756..3323511 (-) 756 WP_000202996.1 zinc ABC transporter ATP-binding protein ZnuC -
  AM384_RS16950 (AM384_16885) znuA 3323590..3324522 (+) 933 WP_001342995.1 zinc ABC transporter substrate-binding protein ZnuA -
  AM384_RS16955 (AM384_16890) mepM 3324538..3325860 (+) 1323 WP_001184054.1 murein DD-endopeptidase MepM -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 37173.77 Da        Isoelectric Point: 4.7818

>NTDB_id=197752 AM384_RS16935 WP_000568519.1 3320720..3321730(+) (ruvB) [Escherichia coli strain AR_0137]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TTRAWNHFGITPPEMP

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=197752 AM384_RS16935 WP_000568519.1 3320720..3321730(+) (ruvB) [Escherichia coli strain AR_0137]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTAGCAGATCGCGCCATTCGCCCTAAATT
ACTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCACAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATCTGTTGATTTTTGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCCAACATTGTCGCCAATGAAATGGGC
GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGCGATCTGGCAGCGATGCTCACTAACCTTGAACCGCA
TGACGTGCTGTTTATTGATGAGATCCACCGTCTATCGCCAGTTGTTGAAGAAGTGCTGTACCCGGCAATGGAAGACTACC
AACTGGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACCCTGATTGGTGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGTTGCGCGACCGTTTTGGTATTGTGCAACGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGCGCGCTGGAAGTTGCTC
GTCGCGCTCGCGGTACGCCACGTATTGCCAACCGTCTGCTGCGTCGAGTGCGTGATTTCGCCGAAGTGAAGCACGATGGC
ACCATCTCGGCAGATATCGCTGCTCAGGCGCTGGATATGTTGAATGTCGATGCTGAAGGTTTCGATTATATGGACCGCAA
ATTGTTGCTGGCGGTAATCGATAAGTTCTTTGGTGGGCCGGTAGGTCTGGATAACCTGGCGGCAGCCATTGGCGAAGAAC
GTGAAACCATTGAGGATGTGCTGGAGCCTTATCTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGTGGGCGTATGGCG
ACGACGCGGGCGTGGAATCACTTTGGCATAACGCCGCCAGAAATGCCGTAA

Domains

Predicted by InterProScan.

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(23-181)

RuvB AAA lid domain (AAA_lid_4)

(184-257)

RuvB C-terminal winged helix domain (WHD_RuvB)

(259-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q32HA1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

60.486

97.917

0.592
  ruvB Streptococcus pneumoniae TIGR4

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae R6

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae D39

58.934

94.94

0.56
  ruvB Synechocystis sp. PCC 6803

53.251

96.131

0.512
  ruvB Helicobacter pylori 26695

52.038

94.94

0.494

Multiple sequence alignment