Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   Q5W_RS21140 Genome accession   NZ_CP017311
Coordinates   4452969..4454570 (-) Length   533 a.a.
NCBI ID   WP_009520548.1    Uniprot ID   A0A1L1PIY8
Organism   Hydrogenophaga sp. PBC     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4447969..4459570
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q5W_RS21115 (Q5W_21115) - 4448380..4448583 (-) 204 WP_009520543.1 hypothetical protein -
  Q5W_RS21120 (Q5W_21120) - 4448742..4450364 (+) 1623 WP_009520544.1 FMN-binding glutamate synthase family protein -
  Q5W_RS21125 (Q5W_21125) - 4450380..4451267 (-) 888 WP_009520545.1 hypothetical protein -
  Q5W_RS21130 (Q5W_21130) - 4451264..4451689 (-) 426 WP_009520546.1 putative toxin-antitoxin system toxin component, PIN family -
  Q5W_RS21135 (Q5W_21135) - 4451692..4452972 (-) 1281 WP_009520547.1 THUMP domain-containing protein -
  Q5W_RS21140 (Q5W_21140) rpoN/rpoN1 4452969..4454570 (-) 1602 WP_009520548.1 RNA polymerase factor sigma-54 Machinery gene
  Q5W_RS21145 (Q5W_21145) lptB 4454590..4455378 (-) 789 WP_009520549.1 LPS export ABC transporter ATP-binding protein -
  Q5W_RS21150 (Q5W_21150) lptA 4455375..4455998 (-) 624 WP_009520550.1 lipopolysaccharide transport periplasmic protein LptA -
  Q5W_RS21155 (Q5W_21155) - 4456061..4456681 (-) 621 WP_009520551.1 thiol:disulfide interchange protein DsbA/DsbL -
  Q5W_RS21160 (Q5W_21160) - 4456749..4457444 (-) 696 WP_069903062.1 SPOR domain-containing protein -
  Q5W_RS21165 (Q5W_21165) argS 4457453..4459126 (-) 1674 WP_009520553.1 arginine--tRNA ligase -

Sequence

Protein


Download         Length: 533 a.a.        Molecular weight: 58321.70 Da        Isoelectric Point: 4.5608

>NTDB_id=197582 Q5W_RS21140 WP_009520548.1 4452969..4454570(-) (rpoN/rpoN1) [Hydrogenophaga sp. PBC]
MKQSLSLRVSQHLALTPQLQQSIRLLQLSTLEMAQEVEQMLDENPFLERADETLEREEFGLAATDAPVNAGEQIADAAAP
APSTTADTGDSGDGEAVEANLDGASPVEESWEGDGTVEVAPDDSEWGGDAPPRSTGSGDDGETASAADLARALVSLQDHL
HEQARCLRLSDEDAAVLLFLIESLDDDGYLSDPLSELALALLRLQGDDGNDADSPEATERREACEQLLRTNLQLLQHMDP
PGVGARDLAECLKLQILELRNTPEARAALAICAQPLELIAKRDIRKLVQLCRADEDTVRGALALIARLEPKPGRRFVDVE
RNIVVPDVIVTRAGRGFKVILNPDVMPRLRVHDVYANALRQNRGGRDGGEGGHAAMQQRLQEARWFIKNIQQRFDTILRV
SSAIVERQRNFFMHGELAMRPLVLREIADELGLHESTISRVTTAKYMATPFGTYELKYFFGSSLGTETGGNASSTAVRAL
LKQFIAAEAPAKPLSDNQLSDLLKEQGIECARRTVAKYREAMRIAPANLRKSL

Nucleotide


Download         Length: 1602 bp        

>NTDB_id=197582 Q5W_RS21140 WP_009520548.1 4452969..4454570(-) (rpoN/rpoN1) [Hydrogenophaga sp. PBC]
ATGAAACAGAGTCTTTCCCTTCGCGTCTCTCAGCACCTGGCGCTCACGCCTCAGTTGCAGCAGTCGATACGCCTGCTGCA
ACTGTCGACGCTGGAGATGGCGCAGGAGGTCGAGCAGATGCTCGACGAGAACCCATTTCTTGAACGCGCCGACGAGACGC
TGGAGCGCGAGGAGTTCGGCCTGGCCGCGACCGATGCGCCGGTGAATGCGGGCGAGCAGATCGCCGATGCGGCCGCACCC
GCCCCCAGCACGACCGCCGACACCGGTGACAGCGGAGACGGTGAAGCCGTCGAAGCGAACCTGGACGGCGCCTCGCCGGT
GGAAGAAAGCTGGGAAGGCGACGGCACCGTGGAGGTGGCGCCCGACGACAGCGAGTGGGGTGGCGACGCGCCGCCGCGCA
GCACCGGCTCGGGCGACGACGGCGAGACCGCCAGCGCGGCCGACCTCGCGCGCGCGCTGGTGTCCCTGCAGGACCACCTG
CACGAGCAGGCGCGCTGCCTGCGCCTGTCCGACGAAGACGCCGCCGTGCTGCTCTTCCTGATCGAATCGCTCGACGACGA
CGGCTACCTGAGCGACCCGCTGTCCGAACTCGCGCTCGCGCTGCTGCGCCTGCAAGGCGACGATGGCAACGACGCCGACT
CGCCCGAAGCCACCGAGCGCCGCGAGGCCTGCGAGCAGCTGCTGCGCACCAACCTGCAGTTGCTGCAGCACATGGACCCG
CCCGGCGTGGGTGCGCGCGACCTCGCCGAGTGCCTCAAGCTGCAGATCCTGGAGTTGCGCAACACGCCCGAGGCGCGCGC
GGCGCTGGCCATCTGCGCGCAGCCGCTGGAGCTCATCGCCAAGCGCGACATCCGCAAGCTCGTGCAACTGTGCCGCGCCG
ACGAGGACACCGTGCGCGGCGCGCTCGCGCTCATCGCGCGGCTCGAGCCCAAGCCCGGGCGCCGCTTCGTTGACGTCGAG
CGCAACATCGTCGTGCCCGACGTGATCGTCACGCGCGCCGGGCGCGGCTTCAAGGTCATCCTCAACCCCGACGTGATGCC
CCGCCTGCGCGTGCACGACGTGTACGCCAACGCCCTGCGCCAGAACCGCGGCGGGCGCGATGGCGGCGAGGGCGGCCACG
CCGCCATGCAGCAGCGCCTGCAGGAGGCTCGCTGGTTCATCAAGAACATCCAGCAGCGCTTCGACACCATCCTGCGCGTC
TCCAGCGCCATCGTCGAACGCCAGCGCAACTTCTTCATGCACGGCGAACTCGCCATGCGCCCGCTGGTGCTGCGCGAGAT
CGCTGACGAGCTGGGCCTGCACGAATCCACCATCAGCCGCGTGACCACCGCCAAGTACATGGCCACGCCCTTCGGCACCT
ACGAACTCAAGTACTTCTTCGGTTCCTCGCTCGGCACCGAAACCGGCGGCAACGCCTCCAGCACGGCGGTGCGCGCGCTG
CTCAAGCAGTTCATCGCCGCCGAGGCACCGGCCAAGCCGCTGTCCGACAACCAGCTCTCCGACCTGCTCAAGGAGCAGGG
CATCGAGTGCGCGCGCCGCACCGTGGCCAAGTACCGCGAGGCCATGCGCATCGCCCCGGCCAACCTTCGCAAATCGCTTT
GA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(150-355)

Sigma-54, DNA binding domain (Sigma54_DBD)

(375-531)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1L1PIY8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

51.776

100

0.52

Multiple sequence alignment