Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   BH714_RS19775 Genome accession   NZ_CP017279
Coordinates   4219186..4220619 (-) Length   477 a.a.
NCBI ID   WP_014171817.1    Uniprot ID   A0A839BTX8
Organism   Enterobacter ludwigii strain EN-119     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4214186..4225619
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BH714_RS19745 (BH714_19745) elbB 4215719..4216372 (+) 654 WP_032670144.1 isoprenoid biosynthesis glyoxalase ElbB -
  BH714_RS19750 (BH714_19750) mtgA 4216369..4217094 (+) 726 WP_014171821.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  BH714_RS19755 (BH714_19755) npr 4217133..4217405 (-) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  BH714_RS19760 (BH714_19760) rapZ 4217402..4218256 (-) 855 WP_014171820.1 RNase adapter RapZ -
  BH714_RS19765 (BH714_19765) ptsN 4218302..4218793 (-) 492 WP_014171819.1 PTS IIA-like nitrogen regulatory protein PtsN -
  BH714_RS19770 (BH714_19770) hpf 4218876..4219163 (-) 288 WP_014171818.1 ribosome hibernation promoting factor -
  BH714_RS19775 (BH714_19775) rpoN/rpoN1 4219186..4220619 (-) 1434 WP_014171817.1 RNA polymerase factor sigma-54 Machinery gene
  BH714_RS19780 (BH714_19780) lptB 4220667..4221392 (-) 726 WP_014171816.1 LPS export ABC transporter ATP-binding protein -
  BH714_RS19785 (BH714_19785) lptA 4221399..4221953 (-) 555 WP_020883526.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  BH714_RS19790 (BH714_19790) lptC 4221922..4222497 (-) 576 WP_014171814.1 LPS export ABC transporter periplasmic protein LptC -
  BH714_RS19795 (BH714_19795) kdsC 4222494..4223060 (-) 567 WP_014171813.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  BH714_RS19800 (BH714_19800) kdsD 4223075..4224061 (-) 987 WP_040018741.1 arabinose-5-phosphate isomerase KdsD -
  BH714_RS19805 (BH714_19805) - 4224075..4225052 (-) 978 WP_020883528.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54206.00 Da        Isoelectric Point: 4.3224

>NTDB_id=197295 BH714_RS19775 WP_014171817.1 4219186..4220619(-) (rpoN/rpoN1) [Enterobacter ludwigii strain EN-119]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQADLHEEVDTQQSQDTEALDTADALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDE
ILESMGDEEIELEEIEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFSKETPWLDEARLIISDHLDLLANHDFRSLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNDRWVVELNSDSIPRLQINQQYASMCTSVRNDSDNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTILSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=197295 BH714_RS19775 WP_014171817.1 4219186..4220619(-) (rpoN/rpoN1) [Enterobacter ludwigii strain EN-119]
ATGAAGCAAGGTTTGCAATTACGGCTCAGCCAACAACTGGCGATGACGCCGCAGCTACAGCAGGCGATTCGCCTGTTGCA
ACTGTCCACCTTAGAACTCCAGCAGGAGCTCCAGCAAGCGCTGGATAGCAATCCGTTGCTGGAGCAAGCCGATCTTCATG
AAGAGGTAGATACCCAGCAGTCGCAGGATACTGAAGCACTCGACACCGCCGATGCGCTCGAACAAAAAGAGATGCCGGAC
GAGCTTCCGCTGGATGCCAGCTGGGATGAAATCTACACCGCCGGAACCCCTTCCGGTACGCGTGCAGACTACCAGGACGA
TGAGCTACCGGTTTATCAGGGTGAAACTACCCAGTCACTGCAGGATTACCTGATGTGGCAGGTGGAACTTACCCCCTTCT
CCGATACCGACCGCGCGATAGCGACATCCATTGTCGATGCCGTGGATGACACCGGTTATCTGACCGTCACGCTGGACGAA
ATTCTGGAAAGCATGGGTGACGAAGAGATTGAACTCGAGGAGATAGAAGCCGTTTTGAAGCGCATTCAGCGTTTCGATCC
GGTTGGGGTGGCCGCAAAAGATCTGCGCGACTGCCTGCTTATCCAACTTTCTCAGTTCAGTAAAGAGACGCCATGGCTTG
ATGAAGCCCGCTTGATCATCAGCGACCATCTGGATCTTCTGGCCAACCACGATTTCCGCAGCCTGATGCGCGTCACGCGC
CTGAAAGAAGAGGTGCTGAAAGAGGCGGTCAATCTGATTCAGTCCCTCGATCCGCGCCCCGGTCAGTCCATTCAGACCAG
CGAACCCGAGTACGTCATCCCGGATGTGCTGGTCAGAAAACACAATGACCGCTGGGTCGTTGAACTGAACTCAGACAGCA
TCCCTCGCCTGCAAATCAACCAGCAGTATGCCTCCATGTGCACCAGCGTGCGTAACGACTCCGATAATCAATACATTCGT
AGCAATTTGCAGGAAGCGCGATGGTTGATCAAAAGTCTGGAGAGCCGAAATGATACGCTGCTCCGCGTAAGCCGCTGTAT
TGTCGAACAGCAGCAGGCGTTCTTTGAGCAGGGCGAAGAGTATATGAAACCGATGGTGCTGGCGGATATCGCCCAGGCCG
TCGAGATGCATGAATCAACCATTTCCCGCGTGACCACGCAGAAGTATCTGCACAGCCCACGCGGTATTTTTGAGCTTAAG
TATTTCTTCTCCAGCCATGTGAATACCGAGGGCGGCGGTGAAGCGTCGTCAACGGCCATTCGCGCCCTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCGAAGCCACTGAGCGACAGTAAGTTAACCACCATACTGTCCGACCAGGGTATTATGGTGG
CACGCCGAACTGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCTAACCAGCGTAAACAGCTGGTCTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.941

100

0.486

Multiple sequence alignment