Detailed information NTG0197285

Bioinformatically predicted

Overview

• Name: comGF/cglF
• Type: Machinery gene
• Locus tag: BB164_RS01320
• Genome accession: NZ_CP021867
• Coordinates: 212261..212683 (+)
• Length: 140 a.a.
• NCBI ID: WP_001264244.1
• Uniprot ID: -
• Organism: Streptococcus agalactiae strain SG-M25
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 207261..217683

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BB164_RS01290 (BB164_01290)	-	208136..210148 (+)	2013	WP_000536261.1	DUF859 family phage minor structural protein	-
BB164_RS01295 (BB164_01295)	-	210162..210488 (+)	327	WP_000404431.1	DUF1366 domain-containing protein	-
BB164_RS01300 (BB164_01300)	-	210517..210675 (+)	159	WP_418287329.1	CD1375 family protein	-
BB164_RS01305 (BB164_01305)	-	210688..210990 (+)	303	WP_000215499.1	hypothetical protein	-
BB164_RS01310 (BB164_01310)	-	210992..211327 (+)	336	WP_001001960.1	phage holin	-
BB164_RS01315 (BB164_01315)	-	211329..212048 (+)	720	WP_000236381.1	peptidoglycan amidohydrolase family protein	-
BB164_RS01320 (BB164_01320)	comGF/cglF	212261..212683 (+)	423	WP_001264244.1	competence type IV pilus minor pilin ComGF	Machinery gene
BB164_RS01325 (BB164_01325)	comGG	212661..213032 (+)	372	WP_000601104.1	competence type IV pilus minor pilin ComGG	Machinery gene
BB164_RS01330 (BB164_01330)	comGH	213147..214121 (+)	975	WP_001008574.1	class I SAM-dependent methyltransferase	Machinery gene
BB164_RS01335 (BB164_01335)	-	214153..215346 (+)	1194	WP_000047534.1	acetate kinase	-
BB164_RS01340 (BB164_01340)	-	215498..215704 (+)	207	WP_000798241.1	helix-turn-helix transcriptional regulator	-
BB164_RS01345 (BB164_01345)	-	215763..215900 (+)	138	WP_001865900.1	hypothetical protein	-
BB164_RS01350 (BB164_01350)	-	215941..216396 (+)	456	WP_000905673.1	hypothetical protein	-
BB164_RS01355 (BB164_01355)	-	216465..217130 (+)	666	WP_000008113.1	CPBP family intramembrane glutamic endopeptidase	-

Sequence

Protein

• Length: 140 a.a.
• Molecular weight: 16234.79 Da
• Isoelectric Point: 8.8708

>NTDB_id=197285 BB164_RS01320 WP_001264244.1 212261..212683(+) (comGF/cglF) [Streptococcus agalactiae strain SG-M25]
MRVLDTLLECLVALVTITGALLVYQGLTKLLAQQIVVMSSSSQSEWVLLTQQLNAEFEGAHLEYLRQNKLYLRKQDKIVT
FGKSNKDDFRKTGYDGRGYQPMVYGLDNCQMSQTKSMVKLVFYFKDGLKRTFYYDFKEET

Nucleotide

• Length: 423 bp

>NTDB_id=197285 BB164_RS01320 WP_001264244.1 212261..212683(+) (comGF/cglF) [Streptococcus agalactiae strain SG-M25]
ATGAGAGTTTTAGATACGCTTTTAGAATGTTTGGTAGCATTGGTTACAATCACAGGAGCTTTACTAGTTTATCAAGGACT
GACAAAATTGTTGGCTCAACAGATAGTAGTGATGTCTTCTTCCAGTCAGTCTGAATGGGTGTTATTAACTCAGCAACTAA
ATGCAGAATTTGAAGGCGCTCATCTGGAATATTTAAGACAGAACAAACTTTATTTACGTAAGCAAGATAAGATTGTAACC
TTTGGCAAATCTAATAAAGATGATTTCCGTAAGACAGGTTATGATGGTCGAGGTTATCAACCAATGGTTTATGGGTTAGA
CAATTGTCAAATGAGTCAGACCAAAAGTATGGTAAAACTTGTTTTTTATTTTAAGGACGGGTTAAAAAGGACATTTTACT
ATGATTTTAAAGAAGAAACTTAA

Domains

Predicted by InterProScan.

• Putative Competence protein ComGF (ComGF) (36-129)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF/cglF	Streptococcus pneumoniae Rx1	41.791	95.714	0.4
comGF/cglF	Streptococcus pneumoniae D39	41.791	95.714	0.4
comGF/cglF	Streptococcus pneumoniae R6	41.791	95.714	0.4
comGF/cglF	Streptococcus pneumoniae TIGR4	41.791	95.714	0.4
comGF	Streptococcus mutans UA159	45.161	88.571	0.4
comGF	Streptococcus mutans UA140	45.161	88.571	0.4
comGF/cglF	Streptococcus mitis NCTC 12261	42.636	92.143	0.393
comGF/cglF	Streptococcus mitis SK321	42.636	92.143	0.393
comGF	Lactococcus lactis subsp. cremoris KW2	39.85	95	0.379